An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
AbstractBiofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method. View Full-Text
Share & Cite This Article
Petrachi, T.; Resca, E.; Piccinno, M.S.; Biagi, F.; Strusi, V.; Dominici, M.; Veronesi, E. An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study. Int. J. Environ. Res. Public Health 2017, 14, 1587.
Petrachi T, Resca E, Piccinno MS, Biagi F, Strusi V, Dominici M, Veronesi E. An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study. International Journal of Environmental Research and Public Health. 2017; 14(12):1587.Chicago/Turabian Style
Petrachi, Tiziana; Resca, Elisa; Piccinno, Maria S.; Biagi, Francesco; Strusi, Valentina; Dominici, Massimo; Veronesi, Elena. 2017. "An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study." Int. J. Environ. Res. Public Health 14, no. 12: 1587.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.