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Int. J. Environ. Res. Public Health 2016, 13(8), 773; doi:10.3390/ijerph13080773

Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika

1,2,†,* , 2,†
,
2
,
2
and
2
1
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 10743, China
2
College of Eco-Environmental Engineering, Qinghai University, Xining 10743, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 23 May 2016 / Revised: 23 July 2016 / Accepted: 26 July 2016 / Published: 1 August 2016
View Full-Text   |   Download PDF [1314 KB, uploaded 1 August 2016]   |  

Abstract

Sperm specific lactate dehydrogenases (LDH-C4) is a lactate dehydrogenase that catalyzes the conversion of pyruvate to lactate. In mammals, Ldh-c was originally thought to be expressed only in testes and spermatozoa. Plateau pika (Ochotona curzoniae), which belongs to the genus Ochotona of the Ochotonidea family, is a hypoxia-tolerant mammal living 3000–5000 m above sea level on the Qinghai-Tibet Plateau, an environment which is strongly hypoxic. Ldh-c is expressed not only in testes and sperm, but also in the somatic tissues of plateau pika. To reveal the effect of hypoxia on pika Ldh-c expression, we investigated the mRNA and protein level of Ldh-c as well as the biochemical index of anaerobic glycolysis in pika somatic tissues at the altitudes of 2200 m, 3200 m and 3900 m. Our results showed that mRNA and protein expression levels of Ldh-c in the tissues of pika’s heart, liver, brain and skeletal muscle were increased significantly from 2200 m to 3200 m, but had no difference from 3200 m to 3900 m; the activities of LDH and the contents of lactate showed no difference from 2200 m to 3200 m, but were increased significantly from 3200 m to 3900 m. Hypoxia up-regulated and maintained the expression levels of Ldh-c in the pika somatic cells. Under the hypoxia condition, plateau pikas increased anaerobic glycolysis in somatic cells by LDH-C4, and that may have reduced their dependence on oxygen and enhanced their adaptation to the hypoxic environment. View Full-Text
Keywords: Ldh-c; plateau pika (Ochotona curzoniae); hypoxia; altitude; Qinghai-Tibetan Plateau Ldh-c; plateau pika (Ochotona curzoniae); hypoxia; altitude; Qinghai-Tibetan Plateau
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MDPI and ACS Style

Wei, D.; Wei, L.; Li, X.; Wang, Y.; Wei, L. Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika. Int. J. Environ. Res. Public Health 2016, 13, 773.

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