Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika
AbstractSperm specific lactate dehydrogenases (LDH-C4) is a lactate dehydrogenase that catalyzes the conversion of pyruvate to lactate. In mammals, Ldh-c was originally thought to be expressed only in testes and spermatozoa. Plateau pika (Ochotona curzoniae), which belongs to the genus Ochotona of the Ochotonidea family, is a hypoxia-tolerant mammal living 3000–5000 m above sea level on the Qinghai-Tibet Plateau, an environment which is strongly hypoxic. Ldh-c is expressed not only in testes and sperm, but also in the somatic tissues of plateau pika. To reveal the effect of hypoxia on pika Ldh-c expression, we investigated the mRNA and protein level of Ldh-c as well as the biochemical index of anaerobic glycolysis in pika somatic tissues at the altitudes of 2200 m, 3200 m and 3900 m. Our results showed that mRNA and protein expression levels of Ldh-c in the tissues of pika’s heart, liver, brain and skeletal muscle were increased significantly from 2200 m to 3200 m, but had no difference from 3200 m to 3900 m; the activities of LDH and the contents of lactate showed no difference from 2200 m to 3200 m, but were increased significantly from 3200 m to 3900 m. Hypoxia up-regulated and maintained the expression levels of Ldh-c in the pika somatic cells. Under the hypoxia condition, plateau pikas increased anaerobic glycolysis in somatic cells by LDH-C4, and that may have reduced their dependence on oxygen and enhanced their adaptation to the hypoxic environment. View Full-Text
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Wei, D.; Wei, L.; Li, X.; Wang, Y.; Wei, L. Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika. Int. J. Environ. Res. Public Health 2016, 13, 773.
Wei D, Wei L, Li X, Wang Y, Wei L. Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika. International Journal of Environmental Research and Public Health. 2016; 13(8):773.Chicago/Turabian Style
Wei, Dengbang; Wei, Linna; Li, Xiao; Wang, Yang; Wei, Lian. 2016. "Effect of Hypoxia on Ldh-c Expression in Somatic Cells of Plateau Pika." Int. J. Environ. Res. Public Health 13, no. 8: 773.
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