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Int. J. Environ. Res. Public Health 2016, 13(4), 359; doi:10.3390/ijerph13040359

Primary Paediatric Bronchial Airway Epithelial Cell in Vitro Responses to Environmental Exposures

1
Child Health, University of Aberdeen, Aberdeen AB25 2ZG, UK
2
Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK
3
Department of Paediatric Anaesthesia, Royal Aberdeen Children’s Hospital, Aberdeen AB25 2ZG, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Helena Solo-Gabriele and Alesia Ferguson
Received: 8 January 2016 / Revised: 7 March 2016 / Accepted: 22 March 2016 / Published: 24 March 2016
(This article belongs to the Special Issue Children’s Exposure to Environmental Contaminants)
View Full-Text   |   Download PDF [776 KB, uploaded 24 March 2016]   |  

Abstract

The bronchial airway epithelial cell (BAEC) is the site for initial encounters between inhaled environmental factors and the lower respiratory system. Our hypothesis was that release of pro inflammatory interleukins (IL)-6 and IL-8 from primary BAEC cultured from children will be increased after in vitro exposure to common environmental factors. Primary BAEC were obtained from children undergoing clinically indicated routine general anaesthetic procedures. Cells were exposed to three different concentrations of lipopolysaccharide (LPS) or house dust mite allergen (HDM) or particulates extracted from side stream cigarette smoke (SSCS). BAEC were obtained from 24 children (mean age 7.0 years) and exposed to stimuli. Compared with the negative control, there was an increase in IL-6 and IL-8 release after exposure to HDM (p ≤ 0.001 for both comparisons). There was reduced IL-6 after higher compared to lower SSCS exposure (p = 0.023). There was no change in BAEC release of IL-6 or IL-8 after LPS exposure. BAEC from children are able to recognise and respond in vitro with enhanced pro inflammatory mediator secretion to some inhaled exposures. View Full-Text
Keywords: children’s health; epithelial cell; environmental exposures; house dust mite; interleukin-8; lipopolysaccharide; tobacco smoke children’s health; epithelial cell; environmental exposures; house dust mite; interleukin-8; lipopolysaccharide; tobacco smoke
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

McInnes, N.; Davidson, M.; Scaife, A.; Miller, D.; Spiteri, D.; Engelhardt, T.; Semple, S.; Devereux, G.; Walsh, G.; Turner, S. Primary Paediatric Bronchial Airway Epithelial Cell in Vitro Responses to Environmental Exposures. Int. J. Environ. Res. Public Health 2016, 13, 359.

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