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Int. J. Environ. Res. Public Health 2015, 12(4), 3633-3645; doi:10.3390/ijerph120403633

Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes

Department of Hygiene and Public Health, Nippon Medical School, Tokyo 113-8602 Japan
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Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 13 February 2015 / Revised: 18 March 2015 / Accepted: 25 March 2015 / Published: 1 April 2015
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Abstract

We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c. View Full-Text
Keywords: annexin-V; apoptosis; carbamate pesticide; carbaryl; caspase; cytochrome-c; Jurkat T cell; maneb; thiram; ziram annexin-V; apoptosis; carbamate pesticide; carbaryl; caspase; cytochrome-c; Jurkat T cell; maneb; thiram; ziram
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, Q.; Kobayashi, M.; Kawada, T. Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes. Int. J. Environ. Res. Public Health 2015, 12, 3633-3645.

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