Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes
AbstractWe previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c. View Full-Text
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Li, Q.; Kobayashi, M.; Kawada, T. Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes. Int. J. Environ. Res. Public Health 2015, 12, 3633-3645.
Li Q, Kobayashi M, Kawada T. Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes. International Journal of Environmental Research and Public Health. 2015; 12(4):3633-3645.Chicago/Turabian Style
Li, Qing; Kobayashi, Maiko; Kawada, Tomoyuki. 2015. "Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes." Int. J. Environ. Res. Public Health 12, no. 4: 3633-3645.