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Int. J. Environ. Res. Public Health 2015, 12(1), 970-985; doi:10.3390/ijerph120100970

Prevalence of Virulence Determinants and Antimicrobial Resistance among Commensal Escherichia coli Derived from Dairy and Beef Cattle

1
Department of Molecular Biology, Faculty of Biological Sciences, University of Zielona Góra, Monte Cassino St. 21b, 65-561 Zielona Góra, Poland
2
Department of Mathematical Statistics and Econometrics, Faculty of Mathematics, Computer Science and Econometrics, University of Zielona Góra, Prof. Z. Szafrana St. 4a, 65-516 Zielona Góra, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 10 October 2014 / Accepted: 22 December 2014 / Published: 19 January 2015
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Abstract

Cattle is a reservoir of potentially pathogenic E. coli, bacteria that can represent a significant threat to public health, hence it is crucial to monitor the prevalence of the genetic determinants of virulence and antimicrobial resistance among the E. coli population. The aim of this study was the analysis of the phylogenetic structure, distribution of virulence factors (VFs) and prevalence of antimicrobial resistance among E. coli isolated from two groups of healthy cattle: 50 cows housed in the conventional barn (147 isolates) and 42 cows living on the ecological pasture (118 isolates). The phylogenetic analysis, identification of VFs and antimicrobial resistance genes were based on either multiplex or simplex PCR. The antimicrobial susceptibilities of E. coli were examined using the broth microdilution method. Two statistical approaches were used to analyse the results obtained for two groups of cattle. The relations between the dependent (VFs profiles, antibiotics) and the independent variables were described using the two models. The mixed logit model was used to characterise the prevalence of the analysed factors in the sets of isolates. The univariate logistic regression model was used to characterise the prevalence of these factors in particular animals. Given each model, the odds ratio (OR) and the 95% confidence interval for the population were estimated. The phylogroup B1 was predominant among isolates from beef cattle, while the phylogroups A, B1 and D occurred with equal frequency among isolates from dairy cattle. The frequency of VFs-positive isolates was significantly higher among isolates from beef cattle. E. coli from dairy cattle revealed significantly higher resistance to antibiotics. Some of the tested resistance genes were present among isolates from dairy cattle. Our study showed that the habitat and diet may affect the genetic diversity of commensal E. coli in the cattle. The results suggest that the ecological pasture habitat is related to the increased spreading rate of the VFs, while the barn habitat is characterised by the higher levels of antimicrobial resistance among E. coli. View Full-Text
Keywords: commensal Escherichia coli; dairy cattle; beef cattle; phylogenetic grouping; virulence factors; antimicrobial resistance commensal Escherichia coli; dairy cattle; beef cattle; phylogenetic grouping; virulence factors; antimicrobial resistance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bok, E.; Mazurek, J.; Stosik, M.; Wojciech, M.; Baldy-Chudzik, K. Prevalence of Virulence Determinants and Antimicrobial Resistance among Commensal Escherichia coli Derived from Dairy and Beef Cattle. Int. J. Environ. Res. Public Health 2015, 12, 970-985.

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