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Int. J. Environ. Res. Public Health 2014, 11(7), 7456-7469; doi:10.3390/ijerph110707456

A Synthetic Thiourea-Based Tripodal Receptor that Impairs the Function of Human First Trimester Cytotrophoblast Cells

1
Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA
2
Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA
3
Department of Pediatrics, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA
*
Author to whom correspondence should be addressed.
Received: 3 December 2013 / Revised: 8 April 2014 / Accepted: 8 April 2014 / Published: 21 July 2014
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Abstract

A synthetic tripodal-based thiourea receptor (PNTTU) was used to explore the receptor/ligand binding affinity using CTB cells. The human extravillous CTB cells (Sw.71) used in this study were derived from first trimester chorionic villus tissue. The cell proliferation, migration and angiogenic factors were evaluated in PNTTU-treated CTB cells. The PNTTU inhibited the CTBs proliferation and migration. The soluble fms-like tyrosine kinase-1 (sFlt-1) secretion was increased while vascular endothelial growth factor (VEGF) was decreased in the culture media of CTB cells treated with ≥1 nM PNTTU. The angiotensin II receptor type 2 (AT2) expression was significantly upregulated in ≥1 nM PNTTU-treated CTB cells in compared to basal; however, the angiotensin II receptor, type 1 (AT1) and vascular endothelial growth factor receptor 1 (VEGFR-1) expression was downregulated. The anti-proliferative and anti-angiogenic effect of this compound on CTB cells are similar to the effect of CTSs. The receptor/ligand affinity of PNTTU on CTBs provides us the clue to design a potent inhibitor to prevent the CTS-induced impairment of CTB cells. View Full-Text
Keywords: angiogenic; cardiotonic steroids; cell signaling; cytotrophoblast; preeclampsia; thiourea angiogenic; cardiotonic steroids; cell signaling; cytotrophoblast; preeclampsia; thiourea
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Horvat, D.; Khansari, M.E.; Pramanik, A.; Beeram, M.R.; Kuehl, T.J.; Hossain, M.A.; Uddin, M.N. A Synthetic Thiourea-Based Tripodal Receptor that Impairs the Function of Human First Trimester Cytotrophoblast Cells. Int. J. Environ. Res. Public Health 2014, 11, 7456-7469.

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