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Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists
School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China
* Author to whom correspondence should be addressed.
Received: 18 November 2013; in revised form: 6 January 2014 / Accepted: 8 January 2014 / Published: 16 January 2014
Abstract: Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.
Keywords: bacterial antagonists; biocontrol; deoxynivalenol; Fusarium graminearum; wheat kernels
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MDPI and ACS Style
Shi, C.; Yan, P.; Li, J.; Wu, H.; Li, Q.; Guan, S. Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists. Int. J. Environ. Res. Public Health 2014, 11, 1094-1105.
Shi C, Yan P, Li J, Wu H, Li Q, Guan S. Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists. International Journal of Environmental Research and Public Health. 2014; 11(1):1094-1105.
Shi, Cuijuan; Yan, Peisheng; Li, Jiafei; Wu, Hanqi; Li, Qianwei; Guan, Shanshan. 2014. "Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists." Int. J. Environ. Res. Public Health 11, no. 1: 1094-1105.