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Genetic Detection of Pseudomonas spp. in Commercial Amazonian Fish
Department of Functional Biology, University of Oviedo. C/Julian Claveria s/n, Oviedo 33006, Spain
Laboratory of Toxicology, National School of Public Health, Fundaçao Oswaldo Cruz, Rio de Janeiro 21041, Brazil
* Author to whom correspondence should be addressed.
Received: 23 June 2013; in revised form: 23 July 2013 / Accepted: 15 August 2013 / Published: 29 August 2013
Abstract: Brazilian freshwater fish caught from large drainages like the River Amazon represent a million ton market in expansion, which is of enormous importance for export to other continents as exotic seafood. A guarantee of bacteriological safety is required for international exports that comprise a set of different bacteria but not any Pseudomonas. However, diarrhoea, infections and even septicaemia caused by some Pseudomonas species have been reported, especially in immune-depressed patients. In this work we have employed PCR-based methodology for identifying Pseudomonas species in commercial fish caught from two different areas within the Amazon basin. Most fish caught from the downstream tributary River Tapajòs were contaminated by five different Pseudomonas species. All fish samples obtained from the River Negro tributary (Manaus markets) contained Pseudomonas, but a less diverse community with only two species. The most dangerous Pseudomonas species for human health, P. aeruginosa, was not found and consumption of these fish (from their Pseudomonas content) can be considered safe for healthy consumers. As a precautionary approach we suggest considering Pseudomonas in routine bacteriological surveys of imported seafood.
Keywords: Amazon River; Pseudomonas; molecular tests; commercial fish; food safety
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MDPI and ACS Style
Ardura, A.; Linde, A.R.; Garcia-Vazquez, E. Genetic Detection of Pseudomonas spp. in Commercial Amazonian Fish. Int. J. Environ. Res. Public Health 2013, 10, 3954-3966.
Ardura A, Linde AR, Garcia-Vazquez E. Genetic Detection of Pseudomonas spp. in Commercial Amazonian Fish. International Journal of Environmental Research and Public Health. 2013; 10(9):3954-3966.
Ardura, Alba; Linde, Ana R.; Garcia-Vazquez, Eva. 2013. "Genetic Detection of Pseudomonas spp. in Commercial Amazonian Fish." Int. J. Environ. Res. Public Health 10, no. 9: 3954-3966.