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Int. J. Environ. Res. Public Health 2013, 10(7), 2897-2905; doi:10.3390/ijerph10072897
Article

Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

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Received: 6 May 2013; in revised form: 17 June 2013 / Accepted: 3 July 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Food Allergy, Genes and Environment)
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Abstract: We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
Keywords: peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Peng, J.; Song, S.; Xu, L.; Ma, W.; Liu, L.; Kuang, H.; Xu, C. Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. Int. J. Environ. Res. Public Health 2013, 10, 2897-2905.

AMA Style

Peng J, Song S, Xu L, Ma W, Liu L, Kuang H, Xu C. Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. International Journal of Environmental Research and Public Health. 2013; 10(7):2897-2905.

Chicago/Turabian Style

Peng, Juan; Song, Shanshan; Xu, Liguang; Ma, Wei; Liu, Liqiang; Kuang, Hua; Xu, Chuanlai. 2013. "Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food." Int. J. Environ. Res. Public Health 10, no. 7: 2897-2905.


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