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Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 6 May 2013; in revised form: 17 June 2013 / Accepted: 3 July 2013 / Published: 12 July 2013
Abstract: We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
Keywords: peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA
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Peng, J.; Song, S.; Xu, L.; Ma, W.; Liu, L.; Kuang, H.; Xu, C. Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. Int. J. Environ. Res. Public Health 2013, 10, 2897-2905.
Peng J, Song S, Xu L, Ma W, Liu L, Kuang H, Xu C. Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. International Journal of Environmental Research and Public Health. 2013; 10(7):2897-2905.
Peng, Juan; Song, Shanshan; Xu, Liguang; Ma, Wei; Liu, Liqiang; Kuang, Hua; Xu, Chuanlai. 2013. "Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food." Int. J. Environ. Res. Public Health 10, no. 7: 2897-2905.