- freely available
Marine Drugs 2011, 9(7), 1254-1272; doi:10.3390/md9071254
Published: 14 July 2011
Abstract: The anti-tumor effects of 11-dehydrosinulariolide, an active ingredient isolated from soft coral Sinularia leptoclados, on CAL-27 cells were investigated in this study. In the MTT assay for cell proliferation, increasing concentrations of 11-dehydrosinulariolide decreased CAL-27 cell viability. When a concentration of 1.5 μg/mL of 11-dehydrosinulariolide was applied, the CAL-27 cells viability was reduced to a level of 70% of the control sample. The wound healing function decreased as the concentration of 11-dehydrosinulariolide increased. The results in this study indicated that treatment with 11-dehydrosinulariolide for 6 h significantly induced both early and late apoptosis of CAL-27 cells, observed by flow cytometric measurement and microscopic fluorescent observation. A comparative proteomic analysis was conducted to investigate the effects of 11-dehydrosinulariolide on CAL-27 cells at the molecular level by comparison between the protein profiling (revealed on a 2-DE map) of CAL-27 cells treated with 11-dehydrosinulariolide and that of CAL-27 cells without the treatment. A total of 28 differential proteins (12 up-regulated and 16 down-regulated) in CAL-27 cells treated with 11-dehydrosinulariolide have been identified by LC-MS/MS analysis. Some of the differential proteins are associated with cell proliferation, apoptosis, protein synthesis, protein folding, and energy metabolism. The results of this study provided clues for the investigation of biochemical mechanisms of the anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells and could be valuable information for drug development and progression monitoring of oral squamous cell carcinoma (OSCC).
Oral squamous cell carcinoma (OSCC), the most widespread malignant neoplasm of the oral cavity, is the sixth most prevalent malignancy worldwide and the fourth most common cancer among the Taiwanese male population . OSCC can be treated by surgical operation, chemotherapy, radiotherapy, and combinations of the treatments; however, patients usually have poor prognosis, i.e., 50% survival rate in 5 years [2,3]. Studies indicated that the metabolite from soft coral such as steroids, terpenoids [4,5], and diterpenes have antimicrobial, antitumor, and HIV-inhibitory activities . Acylspermidines from the Okinawan soft coral, Sinularia sp. expressed strong cytotoxicity against human tumor cell lines along with the induction of apoptotic phenomena . Three norcembrane-based diterpenoid, 1-epi-leptocladolide A, leptocladolides A, and 7E-leptocladolide A have been isolated from soft coral Sinularia leptoclados. They have shown significant cytotoxic activity against KB and Hepa59T/VGH cancer lines . Despite the numerous reports, the mechanisms of anti-cancer effects of the extracts from soft coral are still not well understood.
Analysis of the protein profilings of the cells and tissues by proteomic analysis has been rapidly developed. Proteomic analysis provides a systematic approach to reveal the overall protein expression along with qualitative and quantitative analysis of a specific protein. Comparative proteomic analysis can be used to monitor the up-regulation and down-regulation of proteins under treatments with different compounds in comparison with the control. The results could provide valuable information for drug development and monitoring disease progression [9,10]. In this study, comparative proteomic analysis with two-dimensional gel electrophoresis (2-DE) was performed to investigate the effects of 11-dehydrosinulariolide on the oral caner cells. The chemical structure and the NMR spectra of 11-dehydrosinulariolide were shown in Figure 1. It is an active compound isolated from the soft coral Sinularia leptoclados . Differential proteins were detected by comparing between the overall protein expression of CAL-27 cells treated with 11-dehydrosinulariolide and that of CAL-27 cells without treatment. The biochemical functions of the differential proteins were also explored.
2. Results and Discussion
2.1. The Cytotoxic Effects of 11-Dehydrosinulariolide on CAL-27 Cells
The CAL-27 cells were treated with 11-dehydrosinulariolide of various concentrations (0.5, 1.0, 1.5, 2.0 μg/mL) for 24 h and the viabilities of the cells were determined by MTT assay. (Figure 2) The results indicated that increasing concentrations of 11-dehydrosinulariolide decreased CAL-27 cell viability. When a concentration of 1.5 μg/mL of 11-dehydrosinulariolide was applied, the CAL-27 cell viability was reduced to a level of 70% of the control sample. The morphological changes of the cells treated with 11-dehydrosinulariolide were observed by inverted light microscopy. The untreated cells that grew looked compact and smooth, on the other hand, the cells treated with 1.5 μg/mL of 11-dehydrosinulariolide showed shrinkage and a distinct decreased cell population compared with the counterpart (Figure 3). The effect of 11-dehydrosinulariolide on CAL-27 cell was also examined by wound healing assay. The wound healing function decreased as the concentrations of 11-dehydrosinulariolide increased (Figure 4).
2.2. Apoptosis of CAL-27 Treated with 11-Dehydrosinulariolide
To investigate the cytotoxicity effect of 11-dehydrosinulariolide for the induction of apoptosis, CAL-27 cells were treated with 11-dehydrosinulariolide and analyzed by flow cytometry. The apoptosis rate of 1.75% for CAL-27 was observed after the treatment with 1.5 μg/mL 11-dehydrosinulariolide for 6 h versus the apoptosis rate of 0.2% for the control sample (lower right of Figure 5). The late apoptosis/necrosis cell rate of 3.68% was observed for CAL-27 after the treatment with 1.5 μg/mL 11-dehydrosinulariolide versus the late apoptosis/necrosis cell rate of 0.93% for the control (upper right of Figure 5). These results clearly indicated that treatment with 11-dehydrosinulariolide significantly induced both early and late apoptosis of CAL-27 cells, determined by flow cytometric measurement and microscopic fluorescent observation (Figure 5C).
After treatment with 11-dehydrosinulariolide for 6 h, 1 × 106 adherent cells were trypsinized and incubated with FITC-conjugated annexin V and propidium iodide(PI) for 15 min in the dark. Cells were analyzed by flow cytometry. In the gate areas, the upper right panel was for the population of late apoptotic cells and the lower right panel was for the early apoptotic cells with increased annexin V. The results indicated the treatment with 11-dehydrosinulariolide for 12 h significantly induced early and late apoptosis of CAL-27 cells.
2.3. Proteomic Analysis of CAL-27 Cells Treated with 11-Dehydrosinulariolide
The CAL-27 cells were harvested after being treated with a 1.5 μg/mL solution of 11-dehydrosinulariolide. The proteins were extracted from cultured cells with lysis buffer. The supernatants were collected and the proteins were precipitated by TCA/Acetone. The 2-DE maps of the CAL-27 cells treated with 11-dehydrosinulariolide were compared with those of the control samples to examine the effect of 11-dehydrosinulariolide on CAL-27. The 2-DE were run with a loading of 50 μg protein (pI 4–7) and visualized by silver staining (Figures 6 and 7). PDQuest image analysis software (Bio-Rad) was employed for detecting the differential protein spots which were defined as the proteins showing a more than 1.5 fold intensity difference in 2-DE maps between the treated CAL-27 cells and the control samples. The protein identification was carried out by LC-MS/MS analysis after in gel digestion. MASCOT protein identification search software was used for the identification of the differential protein spots. A total of 28 differential protein spots were successfully identified. A list of the identified proteins with their MASCOT score, MS/MS matched sequences, apparent and theoretical MW, pI, coverage, and fold of change in expression level (up-regulation or down-regulation) were shown in Table 1.
The varied expression of proteins distributed throughout the entire gels indicated that multiple clusters of proteins were involved in the effects of 11-dehydrosinulariolide on CAL-27 cell. A total of 12 differential proteins were up-regulated after 11-dehydrosinulariolide treatment. They were protein SET, protein disulfide isomerase A3 (PDIA3), peptidyl-prolyl cis-trans isomerase A, UPF0160 protein MYG1, SPFH domain-containing protein 2 precursor, nucleolin, neutral alpha-glucosidase AB precursor, prohibitin, translationally-controlled tumor protein, inorganic pyrophosphatase, protein DJ-1, and lactoylglutathione lyase. A total of 16 differential proteins were down-regulated after 11-dehydrosinulariolide treatment. They were reticulocalbin-1, 78 kDa, glucose-regulated protein (GRP78), heterogeneous nuclear ribonucleoprotein F (hnRNP F), 60 kDa heat shock protein (Hsp60), actin-like protein 6A, proteasome subunit beta type 4 precursor, F-actin capping protein subunit beta, purine nucleoside phosphorylase, Xaa-Pro dipeptidase, 60S acidic ribosomal protein P0, ubiquinol-cytochrome-c reductase complex core protein 1 (UQCRC1), 40S ribosomal protein SA, transitional endoplasmic reticulum ATPase, isocitrate dehydrogenase subunit alpha, fructose-bisphosphate aldolase A, and nascent polypeptide-associated complex subunit alpha.
2.4. Validation by Western Blotting
The identities of PDIA3, Hsp60, GRP78, UQCRC1 and prohibitin were further validated by western blotting (Figure 8). The results were in agreement with the 2-DE data.
Comparative proteomic analysis can reveal the changes of protein expression of a proteome with a specific treatment by comparison with the control sample. The data could provide clues for the investigation of the effects of the specific treatment and the further understanding of the mechanisms at molecular level. There were reports about the antimicrobial, antiviral, and cytotoxic properties of soft corals [12–14]. It has also been reported that some chemical compounds from soft coral exert anti-tumor activities [8,15,16]. Most of the studies focused on the cytotoxicity of soft corals. There were fewer reports about the anti-tumor effects of active compounds in soft coral. In this study, the anti-cancer effects of 11-dehydrosinulariolide on CAL-27 cells by MTT assay, flow cytometry, and wound healing assay have been investigated. The proteins related with the effect of 11-dehydrosinulariolide have been identified by comparative proteomic analysis. The inhibitory effect of 11-dehydrosinulariolide on cell growth regarding dose-dependent, cytotoxicity or the induction of apoptosis were also assessed. A total of 28 differential proteins have been identified including 12 up-regulated proteins and 16 down-regulated proteins. It was found that some proteins such as PDIA3, Hsp60, GRP78, hnRNP F, UQCRC1 and prohibitin, are associated with apoptosis or inhibition of cancer cell growth.
2.5.1. Protein Disulfide Isomerase A3 (PDIA3)
Protein disulfide isomerase A3, a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum . It has been reported that PDI is induced by endoplasmic reticulum (ER) stress resulting from interference with Ca2+ homeostasis, inhibition of the formation of disulfide bond, and oxidative stress to relieve the accumulation of misfolded or unfolded proteins in the ER . Study indicated that the accumulation of misfolded proteins in the ER initiates unfolded protein response (UPR) which adjusts ER protein folding capacity to promote cell survival. When homeostasis cannot be reestablished, apoptosis could be induced . In the current study the up-regulation of PDIA3 (spot 10) was a response to ER stress resulting from 11-dehydrosinulariolide treatment. It is speculated the accumulation of misfolded protein caused apoptosis of CAL-27 cells.
2.5.2. 60 kDa Heat Shock Protein (Hsp60)
Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria . Hsp60 exists widely in nature. It acts as chaperones to enhance cell survival under physiological stresses . When Hsp60 is over-expressed, it protects cells from ischemia/hypoxic/oxidative injury to maintain mitochondrial integrity, to suppress mitochondrial membrane permeability, and to inhibit apoptotic and necrotic cell death . It was reported that acute ablation of Hsp60 by small interfering RNA induces mitochondrial dysfunction, destabilizes mitochondrial pool of survivin, and activates both p53-dependent and mitochondrial apoptosis . In this study, the down-regulation of Hsp60 after 11-dehydrosinulariolide treatment is an indication of the anti-cancer effect which may be related with p53-dependent or mitochondrial apoptosis in CAL-27 cells.
2.5.3. 78 kDa Glucose-Regulated Protein (GRP78)
GRP78 (spot 3) was found to be down-regulation by 11-dehydrosinulariolide treated in the study. GRP78 is a constitutively expressed resident protein of the endoplasmic reticulum (ER) in all eukaryotic cells. It is a member of the highly conserved Hsp70 family and is involved in polypeptide translation, protein folding, and protein degradation . Studies indicated that suppression of the stress-mediated induction of GRP78 could accelerate apoptosis, inhibit tumor growth, and increase the cytotoxicity of chronically hypoxic cells [25–27]. Down-regulation of GRP78 caused by interfering RNA leads to a slowdown of cell growth in glioma . It has been reported that GRP78 could inhibit cytochrome c-mediated caspase activation in a cell-free system and caspase-mediated cell death . It is speculated that the expression of GRP78 after the 11-dehydrosinulariolide treatment is related with caspase activation or ER stress response in CAL-27 cells.
2.5.4. Heterogeneous Nuclear Ribonucleoprotein F (hnRNP F)
HnRNP F is highly expressed in tumor cells except in hepatocellular carcinoma . It is a member of the hnRNP family which plays roles in transcription, RNA editing, and translation. HnRNP F is capable of catalyzing splicing events in both viral [31–34] and nonviral transcripts [35–37] and modulating the splicing of apoptotic regulator Bcl-x . The elevated expression of Bcl-XL is related to decreased apoptosis in cancer cells, increased risk of metastasis, increased resistance to chemotherapeutic drugs, and poor clinical outcome [38,39]. On the other hand, Bcl-Xs can induce apoptosis and help sensitize cells to chemotherapeutic agents .
In this study, the down-regulation of hnRNP F (spot 4) in CAL-27 cells after 11-dehydrosinulariolide treatment might be correlated with decreased expression of Bcl-XL and increased apoptosis.
Prohibitins are evolutionary conserved proteins that are localized in mitochondria. They are also involved in transcriptional regulation [40,41], apoptosis [42,43], regulation of cellular signal , and mitochondrial biogenesis [45,46]. Translocation of prohibitin to mitochondria accompanies a simultaneous translocation of the p53 protein. It has been reported that p53 can translocate to the mitochondria in response to apoptosis [47–49]. It has been reported that prohititin has the potential to be an anti-proliferative protein, a tumor suppressor, or a regulator for cell progression and apoptosis . The proteomic data in this study indicated that the expression of prohibitin was up-regulated in CAL-27 cells treated with 11-dehydrosinulariolide. This up-regulation is possibly associated with the induction of p53 export and the subsequent apoptosis.
2.5.6. Ubiquinol Cytochrome c Reductase Complex Core Protein 1 (UQCRC1)
The expression of UQCRC1 (spot 16) was down-regulated in CAl-27 cells treated with 11-dehydrosinulariolide. UQCRC1, a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1 . Complex III is responsible for generating an electrochemical potential coupled to ATP synthesis for transferring electrons from ubiquinol to cytochrome c. There were repots indicated that some mitochondrial myopathies were caused by deficiency of complex III [52,53]. Dysfunction of mitochondrial can result in excessive production of reactive oxygen species to stimulate the release of chtochrome c from mitochondrial intermembrane space to the cytosol and to trigger the transduction pathway of apoptosis [54,55]. It has been reported that mitochondrial oxygen radical formation could be regulated by the uniquinol/bcl redox couple . The amplification or over-expression of UQCRC1 might create a stressful cellular environment. Energetic metabolism is another function of the mitochondrial respiratory chain. Therefore, amplification or over-expression of UQCRC1 might contribute to the rapid proliferation of cancer cells by rapid synthesis of ATP. The functions of some anticancer drugs are to inhibit electron transport in mitochondria at the levels of complex III [57,58]. Since UQCRC1 is a key subunit of complex III, modulation of this protein may contribute to cancer characteristics. Based on the results of this study, it is proposed that increased concentration of 11-dehydrosinulariolide resulted in a progressive inhibition of O2 uptake and decreased UQCRC1 expression in CAL-27 cells and, consequently, caused mitochondria dysfunction and subsequently triggered the transduction pathway of apoptosis.
3. Experimental Section
3.1. Cell Culture and Treatment with 11-Dehydrosinulariolide
CAL-27 cells were grown in DMEM with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, supplemented with 10% (v/v) FBS, 100 units/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 0.01 mg/mL human transferring in a humidified atmosphere with 5% CO2 in air at 37 °C. When cells reached above 70% confluency, subculture was conducted at a split ratio of 1:6. CAL-27 cells were cultured in a 10 cm dish for each assay. The isolation of 11-dehydrosinulariolide from the soft coral Sinularia leptoclado was carried out according to the literature procedures . Control cultures were made by adding DMSO at the same final concentration as in the treated samples (0.01% v/v). Cells were added with four different concentrations of 11-dehydrosinulariolide (0.5 μg/mL, 1.0 μg/mL, 1.5 μg/mL, and 2.0 μg/mL) and harvested after 24 h incubation. All the experiments were repeated three times to confirm reproducibility.
3.2. MTT Assay
The cytotoxic effect of 11-dehydrosinulariolide against CAL-27 cells was determined by the MTT assay. CAL-27 cells (1 × 105/cm2) were incubated in 96 well microtiter plates. Treatment with various concentrations of 11-dehydrosinulariolide (0.5 μg/mL, 1.0 μg/mL, 1.5 μg/mL, and 2.0 μg/mL) followed for 24 h. Each well was added with 50 μL MTT solution (1 mg/mL in PBS) and the plates were incubated for 4 h at 37 °C. To achieve solubility of purple-blue MTT formazan crystals in viable cells, 200 μL of DMSO was aged to each well. The absorbance was read at 595 nm on a microtiter plate ELISA reader with DMSO as blank. Analysis of variance (ANOVA) followed by the Tukey-Kramer test on GraphPad InStat 3 (San Diego, CA, USA) to determine whether there were significant differences (p ≤ 0.05) .
3.3. Apoptosis and Cell Cycle Analysis
Apoptosis and cell cycle arrest induced by 11-dehydrosinulariolide for 6 h were determined by using an Annexin V-FITC and PI Apoptosis Detection Kit (BioVision, USA ) according to manufacturer’s instructions. Assessment of samples was accomplished FACSauto flow cytometer (Becton Dickinson, USA). Data analysis was carried out with WinMDI software (ver. 2.9, windows Multiple Document Interface, Flow Cytometry Application). The cells in the FITC-positive and PI-negative fractions were defined as apoptotic cells.
3.4. Wound Healing Assay
CAL-27 cells were plated in a 6-well plate. After the cells grew to confluence, a scratch/wound was made with a pipette tip in each of the wells. Cells were washed with PBS and refreshed with FBS-containing medium. Microscopic fields were photographed at the initial point (0 h) and thereafter.
3.5. Protein Extraction and Estimation
CAL-27 cells were untreated or treated with different concentrations of 11-dehydrosinulariolide (0, 0.5, 1.0 and 1.5 μg/mL) for 24 h and then lysed with Cell Extraction Buffer (BioSource International, Camarillo, CA, USA) and protease inhibitor cocktail (Sigma). The total protein in the supernatant was then precipitated out for overnight (−20 °C) by triple the volume of 10% TCA/Acetone solution containing 20 mM DTT. After centrifugation at 8000 rpm for 30 min at 4 °C, the supernatant was discarded. The pellet was suspended in a rehydration buffer (6 M urea, 2 M thiourea, 0.5% CHAPS, 0.5% IPG buffer, 20 mM DTT, and 0.002% bromophenol blue) at 4 °C for overnight. The IPG buffer was purchased from GE Healthcare. The protein contents were determined using 2-D Quant Kit (GE Healthcare).
3.6. Two-Dimensional Gel Electrophoresis
The first dimension electrophoresis (isoelectric focusing) was performed on GE Healthcare Ettan IPGphor 3 at 20 °C with a current limit of 30 A per strip. A sample was dissolved in a rehydration buffer as described above and applied on an IPG strip in a strip holder. Every 11-cm IPG strip (pI 4–7, Immobiline DryStrip) was rehydrated at 30 V for 12 h and then focused according to the preset program: 200 V (2 h), 500 V (2 h), 1000 V (2 h), 4000 V (1 h), 8000 V (4 h), until the total Vh reached 39,400. The equilibrated strip was placed on the top of a SDS-PAGE gel (12.5%), sealed with 0.5% agarose, then the second dimension electrophoresis was run at 150 V for 6.5 h. Electrophoretic unit used for the second dimension electrophoresis was SE 600 Ruby (Hoeffer). 2-DE images were made in triplicate for each sample and normalized prior to statistical analysis.
3.7. Protein Identification by LC-MS/MS
In-gel Digestion: A protein spot of interest was excised into a piece of 1 mm × 1 mm, then placed in a microcentrifuge tube. A 100 μL of 50 mM DTT in 25 mM ammonium bicarbonate (pH 8.5) was added to the tube which was shaken at 37 °C for 1 h. After removing excess DTT in supernatant, a 100 μL of 100 mM Iodoacetamide (IAA) in 25 mM ammonium bicarbonate (pH 8.5) was added to the tube which was then shaken for 30 min at RT in dark. The excess IAA in supernatant was removed. A 100 μL of 50% acetonitrile in 25 mM ammonium bicarbonate buffer (pH 8.5) was added and then the gel piece was soaked for 15 min followed a complete removal of the buffer. A 0.1 μg of trypsin in 10 μL 25 mM ammonium bicarbonate (pH 8.5) was added to the gel piece. Digestion was run for 16 h at 37 °C. The solution was sonicated for 20 s to release the tryptic peptides from the gel. The peptide solution was then concentrated for the LC-MS/MS analysis.
LC-MS/MS analysis: A peptide mixture was separated by nanoflow reversed phase C18 chromatography on nano LC using the Agilent 1200 System and PepMap100 C18, 75 μm × 15 cm (3 μm) nanoLC column or HPLC using the Agilent NanoLC 1200 System and Agilent Zobax 2.1 mm × 150 mm C18 column. LC-MS/MS analysis employed a 10 min online trapping and desalting step followed by a 60 min 5–40% mobile B gradient at nano flow and a 15 min 5–40% mobile B gradient at higher flow (mobile B = 98% ACN, 0.1% formic acid). Samples were analyzed on the AB SCIEX QTRAP® 5500Q mass spectrometer (Applied Biosystems, CA, USA). The scan range was from m/z 100 to1000 for MS. The raw data was processed into a text file format of WIFF with Analyst 1.5.1.
3.8. Western Blotting Analysis
After SDS-PAGE analysis of the treated samples and the controls under reducing conditions, the proteins on gel were transferred to a PVDF membrane (Millipore) for 1.5 h at 400 mA using Transphor TE 62 (Hoeffer). The membranes were then incubated with rabbit polyclonal antibodies against human GRP-78, Hsp60, PDIA3, UQCRC1 and GAPDH (ProteinTech Group, USA) and rabbit monoclonal antibody anti human prohibitin(Epitomics, California, USA) at 4 °C for 2 h. The membranes were washed three times in PBST (10 mM NaH2PO4, 130 mM NaCl, 0.05% Tween 20), then probed with the second Ab (goat anti-rabbit and horseradish peroxidase conjugate, 1:5000 in blocking solution) for 1 h. After it was washed with PBST for three times, the enzyme activity on the blot was visualized through chemiluminesence by adding ECL Western Blotting Reagents (Pierce).
The anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells have been extensively investigated in this study. In the MTT assay for cell proliferation, the inhibition ratio increased as the concentration of 11-dehydrosinulariolide increased. When a concentration of 1.5 μg/mL of 11-dehydrosinulariolide was added to CAL-27 cells, the viability of cells was reduced to a level of 70% of the control. The effect of 11-dehydrosinulariolide on CAL-27 cell was also examined by wound healing assay. The wound healing function decreased as the concentration of 11-dehydrosinulariolide increased. The cytotoxicity effect of 11-dehydrosinulariolide for the induction of apoptosis of CAL-27 cells has also been studied. The results indicated that treatment with 11-dehydrosinulariolide significantly induced both early and late apoptosis of CAL-27 cells, observed by flow cytometric measurement and microscopic fluorescent observation. Comparative proteomic analysis provided an extensive and effective approach to detect changes of protein expression profiling of CAL-27 cells treated with 11-dehydrosinulariolide. A total of 28 differential proteins (12 up-regulated and 16 down-regulated) in the CAL-27 cells treated with 11-dehydrosinulariolide have been identified by LC-MS/MS analysis. The differential proteins, which were defined as the proteins showing a more than 1.5 fold intensity difference in the 2-DE maps between the treated CAL-27 cells and the control samples, including protein disulfide-isomerase A3, 78 kDa glucose-regulated protein, peptidyl-prolyl cis-trans isomerase A, prohibitin, heterogeneous nuclear ribonucleoprotein F, 60 kDa heat shock protein, ubiquinol cytochrome c reductase complex core protein 1, 40S ribosomal protein SA, transitional endoplasmic reticulum ATPase, fructose-bisphosphate aldolase A, and nascent polypeptide-associated complex subunit alpha. Some differential proteins are associated with cell proliferation, apopotosis, protein synthesis, protein folding, and energy metabolism.
The results of this study indicated that 11-dehydrosinulariolide could inhibit CAL-27 cell proliferation by exerting effects on regulating the expressions of some specific proteins. Identification and characterization of some functionally modulated proteins in anti-cell-proliferation could help understand the long-term effects of 11-dehydrosinulariolide on CAL-27 cells at the molecular level. Further studies of these proteins will help unveil the anti-tumor mechanisms of the treatment with 11-dehydrosinulariolide and thus benefit the development of new target drugs for oral squamous cell carcinoma (OSCC).
This study was supported in part by a grant from the Meiho University Research Fund (Project No. AMH-99-DBS-006).
- Samples Availability: Available from the authors.
- Chen, Y; Chang, J; Liao, C; Wang, H; Yen, T; Chiu, C; Lu, Y; Li, H; Cheng, A. Head and neck cancer in the betel quid chewing area: Recent advances in molecular carcinogenesis. Cancer Sci 2008, 99, 1507–1514, doi:10.1111/j.1349-7006.2008.00863.x. 18754860
- Liao, C; Chang, J; Wang, H; Ng, S; Hsueh, C; Lee, L; Lin, C; Chen, I; Huang, S; Cheng, A. Analysis of risk factors of predictive local tumor control in oral cavity cancer. Ann. Surg. Oncol 2008, 15, 915–922, doi:10.1245/s10434-007-9761-5. 18165878
- Liao, C; Kang, C; Chang, J; Wang, H; Ng, S; Hsueh, C; Lee, L; Lin, C; Cheng, A. Survival of second and multiple primary tumors in patients with oral cavity squamous cell carcinoma in the betel quid chewing area. Oral Oncol 2007, 43, 811–819, doi:10.1016/j.oraloncology.2006.10.003. 17174143
- Faulkner, DJ. Marine natural products. Nat. Prod. Rep 2002, 19, 1–48. 11902436
- Ruggieri, G. Drugs from the sea. Science 1976, 194, 491–497, doi:10.1126/science.9691. 9691
- Faulkner, DJ. Marine natural products. Nat. Prod. Rep 2001, 18, 1–49, doi:10.1039/b006897g. 11245399
- Ojika, M; Islam, M; Shintani, T; Zhang, Y; Okamoto, T; Sakagami, Y. Three new cytotoxic acylspermidines from the soft coral, Sinularia sp. Biosci. Biotechnol. Biochem 2003, 67, 1410–1412, doi:10.1271/bbb.67.1410. 12843674
- Ahmed, A; Shiue, R; Wang, G; Dai, C; Kuo, Y; Sheu, J. Five novel norcembranoids from Sinularia leptoclados and S. parva. Tetrahedron 2003, 59, 7337–7344, doi:10.1016/S0040-4020(03)01138-4.
- He, Q; Chen, J; Kung, H; Yuen, A; Chiu, J. Identification of tumor associated proteins in oral tongue squamous cell carcinoma by proteomics. Proteomics 2004, 4, 271–278, doi:10.1002/pmic.200300550. 14730689
- Herrmann, PC; Liotta, L; Petricoin, EF, III. Cancer proteomics: The state of the art. Dis Markers 2001, 17, 49–57. 11673651
- Hsieh, PW; Chang, FR; McPhail, AT; Lee, KH; Wu, YC. New cembranolide analogues from the formosan soft coral Sinularia flexibilis and their cytotoxicity. Nat. Prod. Res 2003, 17, 409–418, doi:10.1080/14786910310001617677. 14577690
- Aceret, TL; Coll, JC; Uchio, Y; Sammarco, PW. Antimicrobial activity of the diterpenes flexibilide and sinulariolide derived from Sinularia flexibilis Quoy and Gaimard 1833 (Coelenterata: Alcyonacea, Octocorallia). Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol 1998, 120, 121–126, doi:10.1016/S0742-8413(98)00032-2. 9827024
- Rashid, MA; Gustafson, KR; Boyd, MR. HIV-inhibitory cembrane derivatives from a Philippines collection of the soft coral Lobophytum species. J. Nat. Prod 2000, 63, 531–533, doi:10.1021/np990372p. 10785433
- Ahmed, AF; Hsieh, YT; Wen, ZH; Wu, YC; Sheu, JH. Polyoxygenated sterols from the Formosan soft coral Sinularia gibberosa. J. Nat. Prod 2006, 69, 1275–1279, doi:10.1021/np0601509. 16989519
- Su, JH; Tseng, YJ; Huang, HH; Ahmed, AF; Lu, CK; Wu, YC; Sheu, JH. 9,11-Secosterols from the soft corals Sinularia lochmodes and Sinularia leptoclados. J. Nat. Prod 2006, 69, 850–852, doi:10.1021/np060031t. 16724859
- Ahmed, AF; Tai, SH; Wu, YC; Sheu, JH. Sinugrandisterols AD, trihydroxysteroids from the soft coral Sinularia grandilobata. Steroids 2007, 72, 368–374, doi:10.1016/j.steroids.2007.01.001. 17303201
- Freedman, RB; Hirst, TR; Tuite, MF. Protein disulphide isomerase: Building bridges in protein folding. Trends Biochem. Sci 1994, 19, 331–336, doi:10.1016/0968-0004(94)90072-8. 7940678
- Hendershot, L. The ER function BiP is a master regulator of ER function. Mt. Sinai J. Med 2004, 71, 289–297. 15543429
- So, AYL; de la Fuente, E; Walter, P; Shuman, M; Bernales, S. The unfolded protein response during prostate cancer development. Cancer Metastasis Rev 2009, 28, 219–223, doi:10.1007/s10555-008-9180-5. 19172382
- Hartl, F; Hlodan, R; Langer, T. Molecular chaperones in protein folding: The art of avoiding sticky situations. Trends Biochem. Sci 1994, 19, 20–25, doi:10.1016/0968-0004(94)90169-4. 7908149
- Guimaraes, AJ; Frases, S; Gomez, FJ; Zancope-Oliveira, RM; Nosanchuk, JD. Monoclonal antibodies to heat shock protein 60 alter the pathogenesis of Histoplasma capsulatum. Infect. Immun 2009, 77, 1357–1367, doi:10.1128/IAI.01443-08. 19179416
- He, L; Lemasters, JJ. Heat shock suppresses the permeability transition in rat liver mitochondria. J. Biol. Chem 2003, 278, 16755–16760, doi:10.1074/jbc.M300153200. 12611884
- Ghosh, J; Dohi, T; Kang, B; Altieri, D. Hsp60 regulation of tumor cell apoptosis. J. Biol. Chem 2008, 283, 5188–5194. 18086682
- Haas, I. BiP (GRP78), an essential hsp70 resident protein in the endoplasmic reticulum. Cell. Mol. Life Sci 1994, 50, 1012–1020, doi:10.1007/BF01923455.
- Hughes, C; Shen, J; Subjeck, J. Resistance to etoposide induced by three glucose-regulated stresses in Chinese hamster ovary cells. Cancer Res 1989, 49, 4452–4454. 2501025
- Jamora, C; Dennert, G; Lee, A. Inhibition of tumor progression by suppression of stress protein GRP78/BiP induction in fibrosarcoma B/C10ME. Proc. Natl. Acad. Sci. USA 1996, 93, 7690–7694, doi:10.1073/pnas.93.15.7690. 8755537
- Song, M; Park, Y; Lee, J; Park, K. Induction of glucose-regulated protein 78 by chronic hypoxia in human gastric tumor cells through a protein kinase C-ɛ/ERK/AP-1 signaling cascade. Cancer Res 2001, 61, 8322–8330. 11719466
- Pyrko, P; Schonthal, AH; Hofman, FM; Chen, TC; Lee, AS. The unfolded protein response regulator GRP78/BiP as a novel target for increasing chemosensitivity in malignant gliomas. Cancer Res 2007, 67, 9809–9816, doi:10.1158/0008-5472.CAN-07-0625. 17942911
- Rao, RV; Hermel, E; Castro-Obregon, S; del Rio, G; Ellerby, LM; Ellerby, HM; Bredesen, DE. Coupling endoplasmic reticulum stress to the cell death program. J. Biol. Chem 2001, 276, 33869–33874, doi:10.1074/jbc.M102225200. 11448953
- Honore, B; Baandrup, U; Vorum, H. Heterogeneous nuclear ribonucleoproteins F and H/H′ show differential expression in normal and selected cancer tissues. Exp. Cell Res 2004, 294, 199–209, doi:10.1016/j.yexcr.2003.11.011. 14980514
- Fogel, BL; McNally, MT. A cellular protein, hnRNP H, binds to the negative regulator of splicing element from Rous sarcoma virus. J. Biol. Chem 2000, 275, 32371–32378, doi:10.1074/jbc.M005000200. 10934202
- Jacquenet, S; Mereau, A; Bilodeau, PS; Damier, L; Stoltzfus, CM; Branlant, C. A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J. Biol. Chem 2001, 276, 40464–40475, doi:10.1074/jbc.M104070200. 11526107
- Oberg, D; Fay, J; Lambkin, H; Schwartz, S. A downstream polyadenylation element in human papillomavirus type 16 L2 encodes multiple GGG motifs and interacts with hnRNP H. J. Virol 2005, 79, 9254–9269, doi:10.1128/JVI.79.14.9254-9269.2005. 15994820
- McNally, LM; Yee, L; McNally, MT. Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element. J. Biol. Chem 2006, 281, 2478–2488. 16308319
- Garneau, D; Revil, T; Fisette, JF; Chabot, B. Heterogeneous nuclear ribonucleoprotein F/H proteins modulate the alternative splicing of the apoptotic mediator Bcl-x. J. Biol. Chem 2005, 280, 22641–22650, doi:10.1074/jbc.M501070200. 15837790
- Crawford, JB; Patton, JG. Activation of α-tropomyosin exon 2 is regulated by the SR protein 9G8 and heterogeneous nuclear ribonucleoproteins H and F. Mol. Cell. Biol 2006, 26, 8791–8802, doi:10.1128/MCB.01677-06. 17000773
- Yoshida, T; Kokura, K; Makino, Y; Ossipow, V; Tamura, T. Heterogeneous nuclear RNA-ribonucleoprotein F binds to DNA via an oligo (dG)-motif and is associated with RNA polymerase II. Genes Cells 1999, 4, 707–719, doi:10.1046/j.1365-2443.1999.00295.x. 10620016
- Olopade, OI; Adeyanju, MO; Safa, AR; Hagos, F; Mick, R; Thompson, CB; Recant, WM. Overexpression of BCL-x protein in primary breast cancer is associated with high tumor grade and nodal metastases. Cancer J. Sci. Am 1997, 3, 230–237. 9263629
- Clarke, MF; Apel, IJ; Benedict, MA; Eipers, PG; Sumantran, V; Gonzalez-Garcia, M; Doedens, M; Fukunaga, N; Davidson, B; Dick, JE. A recombinant bcl-x s adenovirus selectively induces apoptosis in cancer cells but not in normal bone marrow cells. Proc. Natl. Acad. Sci. USA 1995, 92, 11024–11028, doi:10.1073/pnas.92.24.11024. 7479929
- Montano, MM; Ekena, K; Delage-Mourroux, R; Chang, W; Martini, P; Katzenellenbogen, BS. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc. Natl. Acad. Sci. USA 1999, 96, 6947–6952, doi:10.1073/pnas.96.12.6947. 10359819
- Sun, L; Liu, L; Yang, XJ; Wu, Z. Akt binds prohibitin 2 and relieves its repression of MyoD and muscle differentiation. J. Cell Sci 2004, 117, 3021–3029, doi:10.1242/jcs.01142. 15173318
- Vander Heiden, MG; Choy, JS; VanderWeele, DJ; Brace, JL; Harris, MH; Bauer, DE; Prange, B; Kron, SJ; Thompson, CB; Rudin, CM. Bcl-x L complements Saccharomyces cerevisiae genes that facilitate the switch from glycolytic to oxidative metabolism. J. Biol. Chem 2002, 277, 44870–44876, doi:10.1074/jbc.M204888200. 12244097
- Fusaro, G; Dasgupta, P; Rastogi, S; Joshi, B; Chellappan, S. Prohibitin induces the transcriptional activity of p53 and is exported from the nucleus upon apoptotic signaling. J. Biol. Chem 2003, 278, 47853–47861, doi:10.1074/jbc.M305171200. 14500729
- Rajalingam, K; Wunder, C; Brinkmann, V; Churin, Y; Hekman, M; Sievers, C; Rapp, UR; Rudel, T. Prohibitin is required for Ras-induced Raf-VMEK-VERK activation and epithelial cell migration. Nat. Cell Biol 2005, 7, 837–843, doi:10.1038/ncb1283. 16041367
- Nijtmans, LGJ; de Jong, L; Sanz, MA; Coates, PJ; Berden, JA; Back, JW; Muijsers, AO; van der Spek, H; Grivell, LA. Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins. EMBO J 2000, 19, 2444–2451, doi:10.1093/emboj/19.11.2444. 10835343
- Sanz, MA; Tsang, WY; Willems, EM; Grivell, LA; Lemire, BD; van der Spek, H; Nijtmans, LGJ. The mitochondrial prohibitin complex is essential for embryonic viability and germline function in Caenorhabditis elegans. J. Biol. Chem 2003, 278, 32091–32099, doi:10.1074/jbc.M304877200. 12794069
- Lohrum, MAE; Woods, DB; Ludwig, RL; Balint, E; Vousden, KH. C-terminal ubiquitination of p53 contributes to nuclear export. Mol. Cell. Biol 2001, 21, 8521–8532, doi:10.1128/MCB.21.24.8521-8532.2001. 11713287
- Mihara, M; Erster, S; Zaika, A; Petrenko, O; Chittenden, T; Pancoska, P; Moll, UM. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell 2003, 11, 577–590, doi:10.1016/S1097-2765(03)00050-9. 12667443
- Chipuk, JE; Green, DR. Cytoplasmic p53: Bax and forward. Cell Cycle 2004, 3, 429–431. 15020844
- Mishra, S; Murphy, LC; Nyomba, B; Murphy, LJ. Prohibitin: A potential target for new therapeutics. Trends Mol. Med 2005, 11, 192–197, doi:10.1016/j.molmed.2005.02.004. 15823758
- Trumpower, B; Edwards, CA. Purification of a reconstitutively active iron-sulfur protein (oxidation factor) from succinate. cytochrome c reductase complex of bovine heart mitochondria. J. Biol. Chem 1979, 254, 8697. 224062
- Schapira, AHV; Cooper, JM; Morgan-Hughes, JA; Landon, DN; Clark, JB. Mitochondrial myopathy with a defect of mitochondrial-protein transport. N. Engl. J. Med 1990, 323, 37–42, doi:10.1056/NEJM199007053230107. 2113185
- Slipetz, D; Aprille, J; Goodyer, P; Rozen, R. Deficiency of complex III of the mitochondrial respiratory chain in a patient with facioscapulohumeral disease. Am. J. Hum. Genet 1991, 48, 502–510. 1847791
- Fiskum, G; Starkov, A; Polster, BM; Chinopoulos, C. Mitochondrial mechanisms of neural cell death and neuroprotective interventions in Parkinson’s disease. Ann. N. Y. Acad. Sci 2003, 991, 111–119. 12846980
- Gille, L; Nohl, H. The ubiquinol/bc1 redox couple regulates mitochondrial oxygen radical formation. Arch. Biochem. Biophys 2001, 388, 34–38, doi:10.1006/abbi.2000.2257. 11361137
- Nicolay, K; de Kruijff, B. Effects of adriamycin on respiratory chain activities in mitochondria from rat liver, rat heart and bovine heart. Evidence for a preferential inhibition of complex III and IV. Biochim. Biophys. Acta Bioenerg 1987, 892, 320–330, doi:10.1016/0005-2728(87)90236-2.
- Goormaghtigh, E; Huart, P; Brasseur, R; Ruysschaert, JM. Mechanism of inhibition of mitochondrial enzymatic complex I–III by adriamycin derivatives. Biochim. Biophys. Acta Biomembr 1986, 861, 83–94.
- Tuquet, C; Dupont, J; Mesneau, A; Roussaux, J. Effects of tamoxifen on the electron transport chain of isolated rat liver mitochondria. Cell Biol. Toxicol 2000, 16, 207–219, doi:10.1023/A:1007695308257. 11101003
- Huang, H; Brennan, T; Muir, M; Mason, R. Functional α1 and β2 adrenergic receptors in human osteoblasts. J. Cell. Physiol 2009, 220, 267–275, doi:10.1002/jcp.21761. 19334040
|Table 1. Identities of the differential proteins in CAL-27 cells treated with 11-dehydrosinulariolide analyzed by LC-MS/MS.|
|Spot no.||Protein name||Accessio n no.||Calculate Mr/pI||Peptide matched||Sequence covered %||MASCOT score||Regulation (fold-change) *|
|2||Protein SET (Phosphatase 2A inhibitor I2PP2A)||Q01105||33.47/4.23||5||20||87||+2.2|
|3||78 kDa glucose-regulated protein precursor||P11021||72.28/5.07||8||8||152||−2.0|
|4||Heterogeneous nuclear ribonucleoprotein F||P52597||45.64/5.38||29||35||345||−2.1|
|5||60 kDa heat shock protein||P10809||61.02/5.70||43||46||753||−3.7|
|6||Actin-like protein 6A||O96019||47.43/5.39||19||23||83||−2.2|
|7||Proteasome subunit beta type 4 precursor||P28070||29.18/5.72||6||35||118||−2.1|
|8||F-actin capping protein subunit beta||P47756||31.33/5.36||22||31||242||−2.8|
|9||Peptidyl-prolyl cis-trans isomerase A||P62937||18.0/7.68||2||9||49||+2.3|
|10||Protein disulfide-isomerase A3 precursor||P14136||56.75/5.98||84||57||1168||+2.2|
|11||Purine nucleoside phosphorylase||P00491||32.09/6.45||9||23||208||−6.4|
|12||UPF0160 protein MYG1||Q9HB07||42.41/6.25||2||5||73||+3.8|
|14||SPFH domain-containing protein 2 precursor||O94905||37.81/5.47||17||6||104||+2.2|
|15||60S acidic ribosomal protein P0||P05388||34.25/5.71||88||44||933||−2.7|
|16||Ubiquinol cytochrome c reductase complex core protein 1||P31930||52.61/5.94||23||28||296||−2.6|
|17||40S ribosomal protein SA||P08865||32.83/4.79||72||51||930||−1.8|
|18||Nascent polypeptide-associated complex subunit alpha||Q13765||23.37/4.52||25||32||494||−3.1|
|20||Transitional endoplasmic reticulum ATPase||P55072||89.26/5.14||127||46||1117||−1.6|
|21||Neutral alpha-glucosidase AB precursor||Q14697||106.8/5.74||85||39||988||+3.0|
|22||Isocitrate dehydrogenase subunit alpha||P50213||39.56/6.47||35||28||413||−3.8|
|23||Fructose-bisphosphate aldolase A||P04075||39.39/8.0||103||60||1330||−9.0|
|26||Translationally-controlled tumor protein (TCTP)||P13693||19.58/4.84||35||52||198||+2.0|
*Regulations (fold-changes) of differentially expression proteins are expressed at 24 h treatment of 11-dehydrosinulariolide.
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