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Chemical Structures and Bioactivities of Sulfated Polysaccharides from Marine Algae
Mar. Drugs 2011, 9(2), 224-241; doi:10.3390/md9020224
Article

Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation

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Received: 23 December 2010 / Revised: 21 January 2011 / Accepted: 7 February 2011 / Published: 10 February 2011
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Abstract

Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared to the sterilization methods using gamma and beta radiations. The changes to both the physical and chemical properties of the sterilized exopolysaccharides were analyzed. The use of ethylene oxide can be recommended for the sterilization of polysaccharides as a weak effect on both rheological and structural properties was observed. This low-temperature gas sterilizing process is very efficient, giving a good Sterility Assurance Level (SAL), and is also well suited to large-scale compound manufacturing in the pharmaceutical industry.
Keywords: marine biotechnology; polysaccharides; sterilization procedures; characterization; molecular weight distribution; rheology marine biotechnology; polysaccharides; sterilization procedures; characterization; molecular weight distribution; rheology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rederstorff, E.; Fatimi, A.; Sinquin, C.; Ratiskol, J.; Merceron, C.; Vinatier, C.; Weiss, P.; Colliec-Jouault, S. Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation. Mar. Drugs 2011, 9, 224-241.

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