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• Yamaguchi, Y
• Hasegawa, Y
• Honma, T
• Nagashima, Y
• Shiomi, K
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• Yamaguchi, Y
• Hasegawa, Y
• Honma, T
• Nagashima, Y
• Shiomi, K
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• Hasegawa, Y
• Honma, T
• Nagashima, Y
• Shiomi, K

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Mar. Drugs 2010, 8(12), 2893-2905; doi:10.3390/md8122893

Article

Screening and cDNA Cloning of Kv1 Potassium Channel Toxins in Sea Anemones

Yoshikazu Yamaguchi ¹ , Yuichi Hasegawa ¹ , Tomohiro Honma ^1,2 , Yuji Nagashima ¹  and Kazuo Shiomi ^1,*

¹ Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477, Japan ² Department of Food Nutrition, Tokai University Junior College, Miyamae-cho, Aoi-ku, Shizuoka 420-8511, Japan

* Author to whom correspondence should be addressed.

Received: 2 November 2010; in revised form: 19 November 2010 / Accepted: 1 December 2010 / Published: 2 December 2010

(This article belongs to the Special Issue Ion Channel Inhibiting Marine Toxins)

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Abstract: When 21 species of sea anemones were screened for Kv1 potassium channel toxins by competitive inhibition of the binding of ¹²⁵I-α-dendrotoxin to rat synaptosomal membranes, 11 species (two species of Actiniidae, one species of Hormathiidae, five species of Stichodactylidae and three species of Thalassianthidae) were found to be positive. Furthermore, full-length cDNAs encoding type 1 potassium channel toxins from three species of Stichodactylidae and three species of Thalassianthidae were cloned by a combination of RT-PCR, 3′RACE and 5′RACE. The precursors of these six toxins are commonly composed of signal peptide, propart and mature peptide portions. As for the mature peptide (35 amino acid residues), the six toxins share more than 90% sequence identities with one another and with κ_1.3-SHTX-She1a (Shk) from Stichodactyla helianthus but only 34–63% identities with the other type 1 potassium channel toxins.

Keywords: cDNA cloning; potassium channel toxin; screening; sea anemone

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