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Geoditin A Induces Oxidative Stress and Apoptosis on Human Colon HT29 Cells
School of Chinese Medicine, the Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
School of Biomedical Sciences, Faculty of Medicine, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
* Author to whom correspondence should be addressed.
Received: 15 December 2009; in revised form: 18 January 2010 / Accepted: 19 January 2010 / Published: 19 January 2010
Abstract: Geoditin A, an isomalabaricane triterpene isolated from the marine sponge Geodia japonica, has been demonstrated to dissipate mitochondrial membrane potential, activate caspase 3, decrease cytoplasmic proliferating cell nuclear antigen (PCNA), and induce apoptosis of leukemia cells, but the underlying mechanism remains unclear . In this study, we found fragmentation of Golgi structure, suppression of transferrin receptor expression, production of oxidants, and DNA fragmentation in human colon cancer HT29 cells after treatment with geoditin A for 24 h. This apoptosis was not abrogated by chelation of intracellular iron with salicylaldehyde isonicotinoyl hydrazone (SIH), but suppressed by N-acetylcysteine (NAC), a thiol antioxidant and GSH precursor, indicating that the cytotoxic effect of geoditin A is likely mediated by a NAC-inhibitable oxidative stress. Our results provide a better understanding of the apoptotic properties and chemotherapeutical potential of this marine triterpene.
Keywords: geoditin A; apoptosis; transferrin receptor; oxidative stress
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MDPI and ACS Style
W. K. Cheung, F.; Li, C.; Che, C.-T.; P. L. Liu, B.; Wang, L.; Liu, W.-K. Geoditin A Induces Oxidative Stress and Apoptosis on Human Colon HT29 Cells. Mar. Drugs 2010, 8, 80-90.
W. K. Cheung F, Li C, Che C-T, P. L. Liu B, Wang L, Liu W-K. Geoditin A Induces Oxidative Stress and Apoptosis on Human Colon HT29 Cells. Marine Drugs. 2010; 8(1):80-90.
W. K. Cheung, Florence; Li, Chunman; Che, Chun-Tao; P. L. Liu, Bonnie; Wang, Lijun; Liu, Wing-Keung. 2010. "Geoditin A Induces Oxidative Stress and Apoptosis on Human Colon HT29 Cells." Mar. Drugs 8, no. 1: 80-90.