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• González, M
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Mar. Drugs 2009, 7(4), 803-815; doi:10.3390/md7040803

Article

Recovery of Proteolytic and Collagenolytic Activities from Viscera By-products of Rayfish (Raja clavata)

Miguel Anxo Murado, María del Pilar González and José Antonio Vázquez *

Grupo de Reciclado y Valorización de Materiales Residuales, Instituto de Investigacións Mariñas (CSIC), r/Eduardo Cabello, 6. Vigo-36208, Galicia, Spain

* Author to whom correspondence should be addressed.

Received: 19 November 2009; in revised form: 10 December 2009 / Accepted: 14 December 2009 / Published: 15 December 2009

(This article belongs to the Special Issue Enzymes from the Sea: Sources, Molecular Biology and Bioprocesses)

Download PDF Full-Text [281 KB, uploaded 15 December 2009 11:27 CET]

Abstract: The aim of this work was to study the recovery of proteolytic and collagenolytic activities from rayfish (Raja clavata) viscera wastes. Initially, different parts of the gastrointestinal tract by-products (stomach, duodenum section including pancreas, final intestine) were evaluated. The extracts from proximal intestine yielded the highest values of both enzymatic activities. Optimal conditions for protease activity quantification were established at pH = 6, T = 40 °C and incubation time ≤20 min. The mathematical equation used to model the joint effect of pH and temperature led to maximum activity at pH = 8.66 and 59.4 °C, respectively. Overcooled acetone was found to be best option for recovery of enzymatic activities in comparison with ethanol, PEG-4000, ammonium sulphate and ultrafiltration system. Finally, a simple and systematic protocol of partial purification and total recovery of proteases and collagenases was defined.

Keywords: proteolytic and collagenolytic activity; by-products upgrading; rayfish viscera wastes; pH and temperature effects; mathematical modelling

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