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Mar. Drugs 2009, 7(4), 589-599; doi:10.3390/md7040589
Article

Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells

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Received: 13 October 2009; in revised form: 11 November 2009 / Accepted: 16 November 2009 / Published: 17 November 2009
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Abstract: N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.
Keywords: LPS; RAW 264.7 cells; NF-κB; TNF-α; N,N-didesmethylgrossularine-1; natural product LPS; RAW 264.7 cells; NF-κB; TNF-α; N,N-didesmethylgrossularine-1; natural product
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Oda, T.; Lee, J.-S.; Sato, Y.; Kabe, Y.; Sakamoto, S.; Handa, H.; Mangindaan, R.E.P.; Namikoshi, M. Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells. Mar. Drugs 2009, 7, 589-599.

AMA Style

Oda T, Lee J-S, Sato Y, Kabe Y, Sakamoto S, Handa H, Mangindaan REP, Namikoshi M. Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells. Marine Drugs. 2009; 7(4):589-599.

Chicago/Turabian Style

Oda, Taiko; Lee, Jong-Soo; Sato, Yuta; Kabe, Yasuaki; Sakamoto, Satoshi; Handa, Hiroshi; Mangindaan, Remy E. P.; Namikoshi, Michio. 2009. "Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells." Mar. Drugs 7, no. 4: 589-599.



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