Mar. Drugs 2008, 6(4), 587-594; doi:10.3390/md6040587
Article

Detection of Diarrheic Shellfish Poisoning and Azaspiracids Toxins in Moroccan Mussels: Comparison of LC-MS Method with the Commercial Immunoassay Kit

1 Laboratoire de Biochimie, Faculté des Sciences, Université Chouaib Doukkali El jadida, Marocco 2 IPIMAR, Instituto Nacional de Investigação Agrária e das Pescas, Av. Brasíilia, 1449-006- Lisboa, Portugal
* Author to whom correspondence should be addressed.
Received: 18 January 2008; in revised form: 15 September 2008 / Accepted: 8 October 2008 / Published: 27 October 2008
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Abstract: Diarrheic shellfish poisoning (DSP) is one of recurrent gastrointestinal illnesses in Morocco, resulting from consumption of contaminated shellfish. In order to develop a rapid and reliable technique for toxins detection, we have compared the results obtained by a commercial immunoassay-“DSP-Check” kit” with those obtained by LC-MS. Both techniques are capable of detecting the toxins in the whole flesh extract which was subjected to prior alkaline hydrolysis in order to detect simultaneously the esterified and non esterified toxin forms. The LC-MS method was found to be able to detect a high level of okadaic acid (OA), low level of dinophysistoxin-2 (DTX2), and surprisingly traces of azaspiracids 2 (AZA2) in mussels. This is the first report of a survey carried out for azaspiracids (AZP) contamination of shellfish harvested in the coastal areas of Morocco. The “DSP-Check” kit was found to detect quantitatively DSP toxins in all contaminated samples containing only OA provided that the parent toxins were within the range of detection and was not in an ester form. A good correlation was observed between the two methods when appropriate dilutions were performed. The immunoassay kit appeared to be more sensitive, specific and faster than LC-MS for determination of DSP in total shellfish extract.
Keywords: Diarrheic shellfish poisoning; Okadaic acid; LC/MS; ELISA; Dinophysistoxin 2; Dinophysis spp.; azaspiracids toxins

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MDPI and ACS Style

Elgarch, A.; Vale, P.; Rifai, S.; Fassouane, A. Detection of Diarrheic Shellfish Poisoning and Azaspiracids Toxins in Moroccan Mussels: Comparison of LC-MS Method with the Commercial Immunoassay Kit. Mar. Drugs 2008, 6, 587-594.

AMA Style

Elgarch A, Vale P, Rifai S, Fassouane A. Detection of Diarrheic Shellfish Poisoning and Azaspiracids Toxins in Moroccan Mussels: Comparison of LC-MS Method with the Commercial Immunoassay Kit. Marine Drugs. 2008; 6(4):587-594.

Chicago/Turabian Style

Elgarch, Adra; Vale, Paulo; Rifai, Saida; Fassouane, Aziz. 2008. "Detection of Diarrheic Shellfish Poisoning and Azaspiracids Toxins in Moroccan Mussels: Comparison of LC-MS Method with the Commercial Immunoassay Kit." Mar. Drugs 6, no. 4: 587-594.

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