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Mar. Drugs 2008, 6(2), 291-307; doi:10.3390/md6020291

Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat)

Marine Immunology Program, Center for Shark Research, Mote Marine Laboratory, Sarasota, FL 34236, USA
Chemical Fate and Effects Program, Center for Ecotoxicology, Mote Marine Laboratory, Sarasota, FL 34236, USA
Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, University of South Florida, All Children’s Hospital, St. Petersburg, FL 33701, USA
Author to whom correspondence should be addressed.
Received: 3 January 2008 / Revised: 7 May 2008 / Accepted: 4 June 2008 / Published: 10 June 2008
(This article belongs to the Special Issue Marine Toxins)
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Harmful algal blooms (HABs) of the toxic dinoflagellate, Karenia brevis, produce red tide toxins, or brevetoxins. Significant health effects associated with red tide toxin exposure have been reported in sea life and in humans, with brevetoxins documented within immune cells from many species. The objective of this research was to investigate potential immunotoxic effects of brevetoxins using a leukemic T cell line (Jurkat) as an in vitro model system. Viability, cell proliferation, and apoptosis assays were conducted using brevetoxin congeners PbTx-2, PbTx-3, and PbTx-6. The effects of in vitro brevetoxin exposure on cell viability and cellular metabolism or proliferation were determined using trypan blue and MTT (1-(4,5-dimethylthiazol-2-yl)-3,5- diphenylformazan), respectively. Using MTT, cellular metabolic activity was decreased in Jurkat cells exposed to 5 - 10 μg/ml PbTx-2 or PbTx-6. After 3 h, no significant effects on cell viability were observed with any toxin congener in concentrations up to 10 μg/ml. Viability decreased dramatically after 24 h in cells treated with PbTx-2 or -6. Apoptosis, as measured by caspase-3 activity, was significantly increased in cells exposed to PbTx-2 or PbTx-6. In summary, brevetoxin congeners varied in effects on Jurkat cells, with PbTx-2 and PbTx-6 eliciting greater cellular effects compared to PbTx-3. View Full-Text
Keywords: red tide; brevetoxin; Karenia brevis; immunotoxicity red tide; brevetoxin; Karenia brevis; immunotoxicity

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Walsh, C.J.; Leggett, S.R.; Strohbehn, K.; Pierce, R.H.; Sleasman, J.W. Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat). Mar. Drugs 2008, 6, 291-307.

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