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Mar. Drugs 2018, 16(9), 295; https://doi.org/10.3390/md16090295

Characterization of a Novel PolyM-Preferred Alginate Lyase from Marine Vibrio splendidus OU02

1
Marine College, Shandong University, Weihai 264209, China
2
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
3
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
*
Authors to whom correspondence should be addressed.
Received: 14 July 2018 / Revised: 9 August 2018 / Accepted: 21 August 2018 / Published: 22 August 2018
(This article belongs to the Collection Marine Polysaccharides)
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Abstract

Alginate lyases are enzymes that degrade alginate into oligosaccharides which possess a variety of biological activities. Discovering and characterizing novel alginate lyases has great significance for industrial and medical applications. In this study, we reported a novel alginate lyase, AlyA-OU02, derived from the marine Vibrio splendidus OU02. The BLASTP searches showed that AlyA-OU02 belonged to polysaccharide lyase family 7 (PL7) and contained two consecutive PL7 domains, which was rare among the alginate lyases in PL7 family. Both the two domains, AlyAa and AlyAb, had lyase activities, while AlyAa exhibited polyM preference, and AlyAb was polyG-preferred. In addition, the enzyme activity of AlyAa was much higher than AlyAb at 25 °C. The full-length enzyme of AlyA-OU02 showed polyM preference, which was the same as AlyAa. AlyAa degraded alginate into di-, tri-, and tetra-alginate oligosaccharides, while AlyAb degraded alginate into tri-, tetra-, and penta-alginate oligosaccharides. The degraded products of AlyA-OU02 were similar to AlyAa. Our work provided a potential candidate in the application of alginate oligosaccharide production and the characterization of the two domains might provide insights into the use of alginate of this organism. View Full-Text
Keywords: alginate lyase; polysaccharide lyase family 7; Vibrio splendidus OU02; polyM-preferred; characterization alginate lyase; polysaccharide lyase family 7; Vibrio splendidus OU02; polyM-preferred; characterization
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Zhuang, J.; Zhang, K.; Liu, X.; Liu, W.; Lyu, Q.; Ji, A. Characterization of a Novel PolyM-Preferred Alginate Lyase from Marine Vibrio splendidus OU02. Mar. Drugs 2018, 16, 295.

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