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Mar. Drugs 2018, 16(4), 128; https://doi.org/10.3390/md16040128

Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage

1
Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
3
Department of Chemistry and Chemical Biology, Biomedical Engineering, Biology, Chemical and Biological Engineering, and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
*
Authors to whom correspondence should be addressed.
Received: 22 March 2018 / Revised: 8 April 2018 / Accepted: 10 April 2018 / Published: 14 April 2018
(This article belongs to the Special Issue Marine Drugs Interact with Functional Proteins)
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Abstract

As an important glycosaminoglycan, keratan sulfate (KS) mainly exists in corneal and cartilage, possessing various biological activities. In this study, we purified KS from blue shark (Prionace glauca) cartilage and prepared KS oligosaccharides (KSO) through keratanase II-catalyzed hydrolysis. The structures of KS and KSO were characterized using multi-dimensional nuclear magnetic resonance (NMR) spectra and liquid chromatography-mass spectrometry (LC-MS). Shark cartilage KS was highly sulfated and modified with ~2.69% N-acetylneuraminic acid (NeuAc) through α(2,3)-linked to galactose. Additionally, KS exhibited binding affinity to Ricinus communis agglutinin I (RCA120) in a concentration-dependent manner, a highly toxic lectin from beans of the castor plant. Furthermore, KSO from dp2 to dp8 bound to RCA120 in the increasing trend while the binding affinity of dp8 was superior to polysaccharide. These results define novel structural features for KS from Prionace glauca cartilage and demonstrate the potential application on ricin-antidote exploitation. View Full-Text
Keywords: Prionace glauca cartilage; keratan sulfate; structural characterization; NMR spectra; HILIC-FTMS; Ricinus communis agglutinin I Prionace glauca cartilage; keratan sulfate; structural characterization; NMR spectra; HILIC-FTMS; Ricinus communis agglutinin I
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, Q.; Li, G.; Zhao, X.; Shan, X.; Cai, C.; Zhao, J.; Zhang, F.; Linhardt, R.J.; Yu, G. Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage. Mar. Drugs 2018, 16, 128.

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