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Mar. Drugs 2018, 16(1), 19; doi:10.3390/md16010019

The Bioactive Extract of Pinnigorgia sp. Induces Apoptosis of Hepatic Stellate Cells via ROS-ERK/JNK-Caspase-3 Signaling

Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
Department of General Surgery, Chang Gung Memorial Hospital, Chiayi 613, Taiwan
Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan
Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan
National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan
Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan
Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University & Academia Sinica, Kaohsiung 804, Taiwan
Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan
Department of Anaesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 4 December 2017 / Revised: 30 December 2017 / Accepted: 6 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Development and Application of Herbal Medicine from Marine Origin)
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The activation of hepatic stellate cells (HSCs) is a significant phenomenon during the pathogenesis of liver disorders, including liver cirrhosis and fibrosis. Here, we identified that the extract from a gorgonian coral Pinnigorgia sp. (Pin) induced apoptosis of HSC-T6 cells. Pin inhibited the viability of HSC-T6 cells and increased their subG1 population, DNA fragmentation, caspase-3 activation, and reactive oxygen species (ROS) production in a concentration-dependent manner. The Pin-induced ROS generation and apoptotic effects were significantly reversed by a thiol antioxidant, N-acetylcysteine (NAC). Additionally, Pin induced ERK/JNK phosphorylation and pharmacological inhibition of ERK/JNK rescued the Pin-induced cell death. Pin-activated ERK/JNK were significantly reduced after the administration of NAC; however, the inhibition of ERK/JNK failed to change the Pin-induced ROS production. Similarly, pinnigorgiol A, a pure compound isolated from Pin, elicited ROS production and apoptosis in HSC-T6 cells. The pinnigorgiol A-induced apoptosis was retrained by NAC. Together, it appears that Pin leads to apoptosis in HSC-T6 cells through ROS-mediated ERK/JNK signaling and caspase-3 activation. Pinnigorgiol A serves as a bioactive compound of Pin and may exhibit therapeutic potential by clearance of HSCs. View Full-Text
Keywords: hepatic stellate cells; Pinnigorgia sp.; ROS; apoptosis; caspase-3; MAPK hepatic stellate cells; Pinnigorgia sp.; ROS; apoptosis; caspase-3; MAPK

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kuo, L.-M.; Chen, P.-J.; Sung, P.-J.; Chang, Y.-C.; Ho, C.-T.; Wu, Y.-H.; Hwang, T.-L. The Bioactive Extract of Pinnigorgia sp. Induces Apoptosis of Hepatic Stellate Cells via ROS-ERK/JNK-Caspase-3 Signaling. Mar. Drugs 2018, 16, 19.

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