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Mar. Drugs 2017, 15(9), 293; https://doi.org/10.3390/md15090293

Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production

1
Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, Tunisia
2
Laboratoire de Biotechnologie Végétale Appliquée à l’Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, Tunisia
3
Center for Molecular Biophysics (CBM), CNRS UPR 4301, Orléans 45071, France
4
Laboratoire BRM-PBA Ifremer, Nantes 44311, France
*
Author to whom correspondence should be addressed.
Received: 20 July 2017 / Revised: 27 August 2017 / Accepted: 31 August 2017 / Published: 20 September 2017
(This article belongs to the Collection Bioactive Compounds from Marine Plankton)
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Abstract

The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production. View Full-Text
Keywords: proteome; Dunaliella; 18S rRNA gene; carotenoids; bidimensional gel electrophoresis; mass spectrometry proteome; Dunaliella; 18S rRNA gene; carotenoids; bidimensional gel electrophoresis; mass spectrometry
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Ben Amor, F.; Elleuch, F.; Ben Hlima, H.; Garnier, M.; Saint-Jean, B.; Barkallah, M.; Pichon, C.; Abdelkafi, S.; Fendri, I. Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production. Mar. Drugs 2017, 15, 293.

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