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Mar. Drugs 2017, 15(8), 241; doi:10.3390/md15080241

Isolation and Tissue Distribution of an Insulin-Like Androgenic Gland Hormone (IAG) of the Male Red Deep-Sea Crab, Chaceon quinquedens

Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, 701 E. Pratt Street Columbus Center, Baltimore, MD 21202, USA
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Received: 14 July 2017 / Revised: 27 July 2017 / Accepted: 29 July 2017 / Published: 1 August 2017
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Abstract

The insulin-like androgenic gland hormone (IAG) found in decapod crustaceans is known to regulate sexual development in males. IAG is produced in the male-specific endocrine tissue, the androgenic gland (AG); however, IAG expression has been also observed in other tissues of decapod crustacean species including Callinectes sapidus and Scylla paramamosain. This study aimed to isolate the full-length cDNA sequence of IAG from the AG of male red deep-sea crabs, Chaceon quinquedens (ChqIAG), and to examine its tissue distribution. To this end, we employed polymerase chain reaction cloning with degenerate primers and 5′ and 3′ rapid amplification of cDNA ends (RACE). The full-length ChqIAG cDNA sequence (1555 nt) includes a 366 nt 5′ untranslated region a 453 nt open reading frame encoding 151 amino acids, and a relatively long 3′ UTR of 733 nt. The ORF consists of a 19 aa signal peptide, 32 aa B chain, 56 aa C chain, and 44 aa A chain. The putative ChqIAG amino acid sequence is most similar to those found in other crab species, including C. sapidus and S. paramamosain, which are clustered together phylogenetically. View Full-Text
Keywords: Chaceon quinquedens; cold water species; red deep-sea crab; insulin-like androgenic gland hormone (IAG); androgenic gland Chaceon quinquedens; cold water species; red deep-sea crab; insulin-like androgenic gland hormone (IAG); androgenic gland
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Lawrence, A.; Green, S.; Chung, J.S. Isolation and Tissue Distribution of an Insulin-Like Androgenic Gland Hormone (IAG) of the Male Red Deep-Sea Crab, Chaceon quinquedens. Mar. Drugs 2017, 15, 241.

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