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Mar. Drugs 2017, 15(7), 215; doi:10.3390/md15070215

Selenium-Containing Polysaccharide-Protein Complex in Se-Enriched Ulva fasciata Induces Mitochondria-Mediated Apoptosis in A549 Human Lung Cancer Cells

1,2,†
,
1,2,†
,
1,2
and
1,2,*
1
Institute of Hydrobiology, Jinan University, Jinan 510632, China
2
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms, Guangdong Higher Education Institutes, Guangzhou 510632, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 23 March 2017 / Revised: 18 June 2017 / Accepted: 1 July 2017 / Published: 16 July 2017
(This article belongs to the Collection Marine Compounds and Cancer)
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Abstract

The role of selenium (Se) and Ulva fasciata as potent cancer chemopreventive and chemotherapeutic agents has been supported by epidemiological, preclinical, and clinical studies. In this study, Se-containing polysaccharide-protein complex (Se-PPC), a novel organoselenium compound, a Se-containing polysaccharide-protein complex in Se-enriched Ulva fasciata, is a potent anti-proliferative agent against human lung cancer A549 cells. Se-PPC markedly inhibited the growth of cancer cells via induction of apoptosis which was accompanied by the formation of apoptotic bodies, an increase in the population of apoptotic sub-G1 phase cells, upregulation of p53, and activation of caspase-3 in A549 cells. Further investigation on intracellular mechanisms indicated that cytochrome C was released from mitochondria into cytosol in A549 cells after Se-PPC treatment. Se-PPC induced depletion of mitochondrial membrane potential (ΔΨm) in A549 cells through regulating the expression of anti-apoptotic (Bcl-2, Bcl-XL) and pro-apoptotic (Bax, Bid) proteins, resulting in disruption of the activation of caspase-9. This is the first report to demonstrate the cytotoxic effect of Se-PPC on human cancer cells and to provide a possible mechanism for this activity. Thus, Se-PPC is a promising novel organoselenium compound with potential to treat human cancers. View Full-Text
Keywords: Ulva fasciata; selenium-containing polysaccharide-protein complex; apoptosis; mitochondria; reactive oxygen species Ulva fasciata; selenium-containing polysaccharide-protein complex; apoptosis; mitochondria; reactive oxygen species
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Sun, X.; Zhong, Y.; Luo, H.; Yang, Y. Selenium-Containing Polysaccharide-Protein Complex in Se-Enriched Ulva fasciata Induces Mitochondria-Mediated Apoptosis in A549 Human Lung Cancer Cells. Mar. Drugs 2017, 15, 215.

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