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Mar. Drugs 2017, 15(7), 201; doi:10.3390/md15070201

The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS

1
Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy
2
CNR-Istituto per i Polimeri, Compositi e Biomateriali IPCB–Unità di Catania, 95126 Catania, Italy
3
Department of Biology and Biotechnology "Charles Darwin", Sapienza-University of Rome, 00185 Rome, Italy
4
Marine Molecular Microbiology & Biotechnology, CNR-Institute for Coastal Marine Environment, 98122 Messina, Italy
5
Immanuel Kant Baltic Federal University, 236040 Kaliningrad, Russia
*
Author to whom correspondence should be addressed.
Received: 3 May 2017 / Revised: 12 June 2017 / Accepted: 22 June 2017 / Published: 27 June 2017
(This article belongs to the Special Issue Marine Oligosaccharides and Polysaccharides)
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Abstract

The structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroides lacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS. View Full-Text
Keywords: extremophiles; halophiles; Halobacteroides; deep-sea hypersaline anoxic basins (DHABs); lipopolysaccharide (LPS); innate immunity; lipid A; core oligosaccharide extremophiles; halophiles; Halobacteroides; deep-sea hypersaline anoxic basins (DHABs); lipopolysaccharide (LPS); innate immunity; lipid A; core oligosaccharide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lorenzo, F.D.; Palmigiano, A.; Paciello, I.; Pallach, M.; Garozzo, D.; Bernardini, M.-L.; Cono, V.L.; Yakimov, M.M.; Molinaro, A.; Silipo, A. The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS. Mar. Drugs 2017, 15, 201.

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