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Mar. Drugs 2017, 15(1), 1; doi:10.3390/md15010001

Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08

1,†
,
1,†
,
1,2,* , 1
,
1
and
1,2,*
1
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China
2
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
These authors contributed equally to this paper.
*
Authors to whom correspondence should be addressed.
Academic Editor: Paola Laurienzo
Received: 24 November 2016 / Revised: 19 December 2016 / Accepted: 19 December 2016 / Published: 23 December 2016
(This article belongs to the Collection Marine Polysaccharides)
View Full-Text   |   Download PDF [1834 KB, uploaded 23 December 2016]   |  

Abstract

Unsaturated alginate disaccharides (UADs), enzymatically derived from the degradation of alginate polymers, are considered powerful antioxidants. In this study, a new high UAD-producing alginate lyase, AlySY08, has been purified from the marine bacterium Vibrio sp. SY08. AlySY08, with a molecular weight of about 33 kDa and a specific activity of 1070.2 U/mg, showed the highest activity at 40 °C in phosphate buffer at pH 7.6. The enzyme was stable over a broad pH range (6.0–9.0) and retained about 75% activity after incubation at 40 °C for 2 h. Moreover, the enzyme was active in the absence of salt ions and its activity was enhanced by the addition of NaCl and KCl. AlySY08 resulted in an endo-type alginate lyase that degrades both polyM and polyG blocks, yielding UADs as the main product (81.4% of total products). All these features made AlySY08 a promising candidate for industrial applications in the production of antioxidants from alginate polysaccharides. View Full-Text
Keywords: alginate lyase; thermostability; unsaturated alginate disaccharides; Vibrio sp. alginate lyase; thermostability; unsaturated alginate disaccharides; Vibrio sp.
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MDPI and ACS Style

Li, S.; Wang, L.; Hao, J.; Xing, M.; Sun, J.; Sun, M. Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08. Mar. Drugs 2017, 15, 1.

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