Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08
AbstractUnsaturated alginate disaccharides (UADs), enzymatically derived from the degradation of alginate polymers, are considered powerful antioxidants. In this study, a new high UAD-producing alginate lyase, AlySY08, has been purified from the marine bacterium Vibrio sp. SY08. AlySY08, with a molecular weight of about 33 kDa and a specific activity of 1070.2 U/mg, showed the highest activity at 40 °C in phosphate buffer at pH 7.6. The enzyme was stable over a broad pH range (6.0–9.0) and retained about 75% activity after incubation at 40 °C for 2 h. Moreover, the enzyme was active in the absence of salt ions and its activity was enhanced by the addition of NaCl and KCl. AlySY08 resulted in an endo-type alginate lyase that degrades both polyM and polyG blocks, yielding UADs as the main product (81.4% of total products). All these features made AlySY08 a promising candidate for industrial applications in the production of antioxidants from alginate polysaccharides. View Full-Text
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Li, S.; Wang, L.; Hao, J.; Xing, M.; Sun, J.; Sun, M. Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08. Mar. Drugs 2017, 15, 1.
Li S, Wang L, Hao J, Xing M, Sun J, Sun M. Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08. Marine Drugs. 2017; 15(1):1.Chicago/Turabian Style
Li, Shangyong; Wang, Linna; Hao, Jianhua; Xing, Mengxin; Sun, Jingjing; Sun, Mi. 2017. "Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08." Mar. Drugs 15, no. 1: 1.
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