Next Article in Journal
Protective Effects of Hydrolyzed Nucleoproteins from Salmon Milt against Ethanol-Induced Liver Injury in Rats
Next Article in Special Issue
Chitosan Oligosaccharide Reduces Propofol Requirements and Propofol-Related Side Effects
Previous Article in Journal
First Total Synthesis and Biological Screening of a Proline-Rich Cyclopeptide from a Caribbean Marine Sponge
Previous Article in Special Issue
Fabrication of Gelatin-Based Electrospun Composite Fibers for Anti-Bacterial Properties and Protein Adsorption
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2016, 14(12), 230; doi:10.3390/md14120230

Biological Potential of Chitinolytic Marine Bacteria

1
Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark
2
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Hitoshi Sashiwa
Received: 18 November 2016 / Revised: 7 December 2016 / Accepted: 8 December 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Advances in Marine Chitin and Chitosan II, 2017)
View Full-Text   |   Download PDF [5916 KB, uploaded 16 December 2016]   |  

Abstract

Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential. View Full-Text
Keywords: chitin; chitinases; antifungal; marine bacteria; Pseudoalteromonadaceae; Vibrionaceae chitin; chitinases; antifungal; marine bacteria; Pseudoalteromonadaceae; Vibrionaceae
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Paulsen, S.S.; Andersen, B.; Gram, L.; Machado, H. Biological Potential of Chitinolytic Marine Bacteria. Mar. Drugs 2016, 14, 230.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top