Next Article in Journal
Recombinant Expression and Characterization of α-Conotoxin LvIA in Escherichia coli
Previous Article in Journal
Structures and Activity of New Anabaenopeptins Produced by Baltic Sea Cyanobacteria
Previous Article in Special Issue
Effects of Organic and Inorganic Nitrogen on the Growth and Production of Domoic Acid by Pseudo-nitzschia multiseries and P. australis (Bacillariophyceae) in Culture
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2016, 14(1), 9; doi:10.3390/md14010009

Specific Metabolites in a Phaeodactylum tricornutum Strain Isolated from Western Norwegian Fjord Water

1
Bergen Marine Biobank, Department of Biology, University of Bergen, Thormøhlensgate 53A/B, N-5020 Bergen, Norway
2
Applied Biotechnology, Uni Research Environment, Thormøhlensgate 49B, N-5006 Bergen, Norway
3
Department of Biology, University of Bergen, Thormøhlensgate 53AB, N-5020 Bergen, Norway
4
Department of Chemistry, University of Bergen, Allégaten 42, N-5020 Bergen, Norway
5
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Véronique Martin-Jézéquel
Received: 16 September 2015 / Revised: 14 December 2015 / Accepted: 22 December 2015 / Published: 30 December 2015
(This article belongs to the Special Issue Metabolites in Diatoms)
View Full-Text   |   Download PDF [1168 KB, uploaded 30 December 2015]   |  

Abstract

We have searched for special characteristics in growth, protein expression, fatty acids and volatile organic compounds (VOCs) in a local Phaeodactylum tricornutum Bohlin strain (Bergen Marine Biobank), by comparing it with a common accession strain (CCAP). Differences in growth and expressed proteins were detected between the BMB strain and the CCAP strain, and the BMB strain reached the highest cell densities under the given growth conditions. Fatty acid (FA) analyses showed highest relative eicosapentaenoic acid (EPA) levels in the exponential phase (25.73% and 28.31%), and highest levels of palmitoleic acid (16:1 n-7) in the stationary phase (46.36% and 43.66%) in the BMB and CCAP strain, respectively. The most striking finding of the VOCs analyses was the relatively high levels of ectocarpene, 6-((1E)-butenyl)-1,4-cycloheptadiene, hormosirene, and desmarestene and structurally related compounds, which were exclusively detected in the BMB strain. Many of the VOCs detected in the CCAP and, in particular, in the BMB strain have been reported as antimicrobial agents. We suggest that the array of pheromones and antimicrobial substances could be part of an allelopathic strategy of the BMB strain, dominated by oval cells, thus reflecting the benthic life stage of this morphological form. These findings show the potential for bioactive metabolites in the BMB strain. View Full-Text
Keywords: Phaeodactylum tricornutum; two-dimensional gel electrophoresis (2DGE); volatile organic compounds (VOCs); hormosirene; ectocarpene; fatty acids; eicosapentaenoic acid (EPA) Phaeodactylum tricornutum; two-dimensional gel electrophoresis (2DGE); volatile organic compounds (VOCs); hormosirene; ectocarpene; fatty acids; eicosapentaenoic acid (EPA)
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Prestegard, S.K.; Erga, S.R.; Steinrücken, P.; Mjøs, S.A.; Knutsen, G.; Rohloff, J. Specific Metabolites in a Phaeodactylum tricornutum Strain Isolated from Western Norwegian Fjord Water. Mar. Drugs 2016, 14, 9.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top