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Mar. Drugs 2015, 13(6), 3287-3308; doi:10.3390/md13063287

Production of Chondroitin Sulphate from Head, Skeleton and Fins of Scyliorhinus canicula By-Products by Combination of Enzymatic, Chemical Precipitation and Ultrafiltration Methodologies

Marine Research Institute (IIM-CSIC), Eduardo Cabello, 6. Vigo, Galicia 36208, Spain
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Author to whom correspondence should be addressed.
Academic Editor: Paola Laurienzo
Received: 20 February 2015 / Accepted: 13 May 2015 / Published: 27 May 2015
(This article belongs to the Collection Marine Polysaccharides)
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Abstract

This study illustrates the optimisation of the experimental conditions of three sequential steps for chondroitin sulphate (CS) recovery from three cartilaginous materials of Scyliorhinus canicula by-products. Optimum conditions of temperature and pH were first obtained for alcalase proteolysis of head cartilage (58 °C/pH 8.5/0.1% (v/w)/10 h of hydrolysis). Then, similar optimal conditions were observed for skeletons and fin materials. Enzymatic hydrolysates were subsequently treated with a combination of alkaline hydroalcoholic saline solutions in order to improve the protein hydrolysis and the selective precipitation of CS. Ranges of 0.53–0.64 M (NaOH) and 1.14–1.20 volumes (EtOH) were the levels for optimal chemical treatment depending on the cartilage origin. Finally, selective purification and concentration of CS and protein elimination of samples obtained from chemical treatment, was assessed by a combination of ultrafiltration and diafiltration (UF-DF) techniques at 30 kDa. View Full-Text
Keywords: chondroitin sulphate production; cartilage S. canicula wastes; by-products upgrade; process optimization; response surface methodology chondroitin sulphate production; cartilage S. canicula wastes; by-products upgrade; process optimization; response surface methodology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Blanco, M.; Fraguas, J.; Sotelo, C.G.; Pérez-Martín, R.I.; Vázquez, J.A. Production of Chondroitin Sulphate from Head, Skeleton and Fins of Scyliorhinus canicula By-Products by Combination of Enzymatic, Chemical Precipitation and Ultrafiltration Methodologies. Mar. Drugs 2015, 13, 3287-3308.

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