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Mar. Drugs 2014, 12(5), 3046-3059; doi:10.3390/md12053046
Article

Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163

1
, 1
, 2,†
, 2
 and 1,*
Received: 7 January 2014; in revised form: 4 March 2014 / Accepted: 10 April 2014 / Published: 22 May 2014
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Abstract: Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine and β-carboline derivatives 110, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.
Keywords: co-cultivation; induced metabolites; sponge-associated actinomycetes; NMR fingerprint; bioactivity co-cultivation; induced metabolites; sponge-associated actinomycetes; NMR fingerprint; bioactivity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Dashti, Y.; Grkovic, T.; Abdelmohsen, U.R.; Hentschel, U.; Quinn, R.J. Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Mar. Drugs 2014, 12, 3046-3059.

AMA Style

Dashti Y, Grkovic T, Abdelmohsen UR, Hentschel U, Quinn RJ. Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Marine Drugs. 2014; 12(5):3046-3059.

Chicago/Turabian Style

Dashti, Yousef; Grkovic, Tanja; Abdelmohsen, Usama R.; Hentschel, Ute; Quinn, Ronald J. 2014. "Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163." Mar. Drugs 12, no. 5: 3046-3059.


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