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Mar. Drugs 2013, 11(7), 2431-2443; doi:10.3390/md11072431
Article

Structure of Fucoidan from Brown Seaweed Turbinaria ornata as Studied by Electrospray Ionization Mass Spectrometry (ESIMS) and Small Angle X-ray Scattering (SAXS) Techniques

1,* , 2
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3
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2
 and
1
1 Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau giay, Hanoi, Vietnam 2 Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung vuong, Nha trang, Khanh hoa, Vietnam 3 Faculty of Engineering, Osaka Electro-Communication University, Hatsu-cho 18-8, Neyagawa-shi, Osaka 572-8530, Japan
* Author to whom correspondence should be addressed.
Received: 25 April 2013 / Revised: 4 June 2013 / Accepted: 17 June 2013 / Published: 12 July 2013
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Abstract

The purpose of this study is to elucidate both the chemical and conformational structure of an unfractionated fucoidan extracted from brown seaweed Turbinaria ornata collected at Nha-trang bay, Vietnam. Electrospray ionization mass spectrometry (ESI-MS) was used for determining the chemical structure and small angle X-ray scattering (SAXS) provided conformational of the structure at the molecular level. The results showed that the fucoidan has a sulfate content of 25.6% and is mainly composed of fucose and galactose residues (Fuc:Gal ≈ 3:1). ESIMS analysis suggested that the fucoidan has a backbone of 3-linked α-l-Fucp residues with branches, →4)-Galp(1→ at C-4 of the fucan chain. Sulfate groups are attached mostly at C-2 and sometimes at C-4 of both fucose and galactose residues. A molecular model of the fucoidan was built based on obtained chemical structure and scattering curves estimated from molecular model and observed SAXS measurement were fitted. The results indicated that fucoidan under study has a rod-like bulky chain conformation.
Keywords: fucoidan; Turbinaria ornata; structure; ESIMS; SAXS fucoidan; Turbinaria ornata; structure; ESIMS; SAXS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Thanh, T.T.T.; Tran, V.T.T.; Yuguchi, Y.; Bui, L.M.; Nguyen, T.T. Structure of Fucoidan from Brown Seaweed Turbinaria ornata as Studied by Electrospray Ionization Mass Spectrometry (ESIMS) and Small Angle X-ray Scattering (SAXS) Techniques. Mar. Drugs 2013, 11, 2431-2443.

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