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A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein
Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China
School of Pharmacy, Xuzhou Medical College, Xuzhou 221004, China
Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University Health System, Singapore 119228, Singapore
Agilent Technologies Singapore Pte Ltd., Singapore 117681, Singapore
* Author to whom correspondence should be addressed.
Received: 12 February 2013; in revised form: 26 April 2013 / Accepted: 28 April 2013 / Published: 7 May 2013
Abstract: Active peptide from shark liver (APSL) is a cytokine from Chiloscyllium plagiosum that can stimulate liver regeneration and protects the pancreas. To study the effect of orally administered recombinant APSL (rAPSL) on an animal model of type 2 diabetes mellitus, the APSL gene was cloned, and APSL was expressed in Bombyx mori N cells (BmN cells), silkworm larvae and silkworm pupae using the silkworm baculovirus expression vector system (BEVS). It was demonstrated that rAPSL was able to significantly reduce the blood glucose level in mice with type 2 diabetes induced by streptozotocin. The analysis of paraffin sections of mouse pancreatic tissues revealed that rAPSL could effectively protect mouse islets from streptozotocin-induced lesions. Compared with the powder prepared from normal silkworm pupae, the powder prepared from pupae expressing rAPSL exhibited greater protective effects, and these results suggest that rAPSL has potential uses as an oral drug for the treatment of diabetes mellitus in the future.
Keywords: active peptide from shark liver; Bombyx mori pupae; BmNPV/Bac-to-Bac baculovirus expression system; type 2 diabetes mellitus; oral administration
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Liu, Y.; Chen, Y.; Chen, J.; Zhang, W.; Sheng, Q.; Chen, J.; Yu, W.; Nie, Z.; Zhang, Y.; Wu, W.; Wang, L.; Indran, I.R.; Li, J.; Qian, L.; Lv, Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Mar. Drugs 2013, 11, 1492-1505.
Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Wang L, Indran IR, Li J, Qian L, Lv Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Marine Drugs. 2013; 11(5):1492-1505.
Liu, Yunlong; Chen, Ying; Chen, Jianqing; Zhang, Wenping; Sheng, Qing; Chen, Jian; Yu, Wei; Nie, Zuoming; Zhang, Yaozhou; Wu, Wutong; Wang, Lisha; Indran, Inthrani R.; Li, Jun; Qian, Lian; Lv, Zhengbing. 2013. "A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein." Mar. Drugs 11, no. 5: 1492-1505.