Mar. Drugs 2013, 11(5), 1492-1505; doi:10.3390/md11051492
Article

A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein

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Received: 12 February 2013; in revised form: 26 April 2013 / Accepted: 28 April 2013 / Published: 7 May 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Active peptide from shark liver (APSL) is a cytokine from Chiloscyllium plagiosum that can stimulate liver regeneration and protects the pancreas. To study the effect of orally administered recombinant APSL (rAPSL) on an animal model of type 2 diabetes mellitus, the APSL gene was cloned, and APSL was expressed in Bombyx mori N cells (BmN cells), silkworm larvae and silkworm pupae using the silkworm baculovirus expression vector system (BEVS). It was demonstrated that rAPSL was able to significantly reduce the blood glucose level in mice with type 2 diabetes induced by streptozotocin. The analysis of paraffin sections of mouse pancreatic tissues revealed that rAPSL could effectively protect mouse islets from streptozotocin-induced lesions. Compared with the powder prepared from normal silkworm pupae, the powder prepared from pupae expressing rAPSL exhibited greater protective effects, and these results suggest that rAPSL has potential uses as an oral drug for the treatment of diabetes mellitus in the future.
Keywords: active peptide from shark liver; Bombyx mori pupae; BmNPV/Bac-to-Bac baculovirus expression system; type 2 diabetes mellitus; oral administration
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MDPI and ACS Style

Liu, Y.; Chen, Y.; Chen, J.; Zhang, W.; Sheng, Q.; Chen, J.; Yu, W.; Nie, Z.; Zhang, Y.; Wu, W.; Wang, L.; Indran, I.R.; Li, J.; Qian, L.; Lv, Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Mar. Drugs 2013, 11, 1492-1505.

AMA Style

Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Wang L, Indran IR, Li J, Qian L, Lv Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Marine Drugs. 2013; 11(5):1492-1505.

Chicago/Turabian Style

Liu, Yunlong; Chen, Ying; Chen, Jianqing; Zhang, Wenping; Sheng, Qing; Chen, Jian; Yu, Wei; Nie, Zuoming; Zhang, Yaozhou; Wu, Wutong; Wang, Lisha; Indran, Inthrani R.; Li, Jun; Qian, Lian; Lv, Zhengbing. 2013. "A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein." Mar. Drugs 11, no. 5: 1492-1505.

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