Mar. Drugs 2013, 11(12), 4902-4916; doi:10.3390/md11124902
Article

N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria

1,2email, 2email, 1,2email and 1,2,* email
Received: 13 October 2013; in revised form: 21 November 2013 / Accepted: 21 November 2013 / Published: 9 December 2013
(This article belongs to the Special Issue Compounds from Cyanobacteria)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Cyanobactins are a recently recognized group of ribosomal cyclic peptides produced by cyanobacteria, which have been studied because of their interesting biological activities. Here, we have used a PCR-based approach to detect the N-terminal protease (A) gene from cyanobactin synthetase gene clusters, in a set of diverse cyanobacteria from our culture collection (Laboratory of Ecotoxicology, Genomics and Evolution (LEGE) CC). Homologues of this gene were found in Microcystis and Rivularia strains, and for the first time in Cuspidothrix, Phormidium and Sphaerospermopsis strains. Phylogenetic relationships inferred from available A-gene sequences, including those obtained in this work, revealed two new groups of phylotypes, harboring Phormidium, Sphaerospermopsis and Rivularia LEGE isolates. Thus, this study shows that, using underexplored cyanobacterial strains, it is still possible to expand the known genetic diversity of genes involved in cyanobactin biosynthesis.
Keywords: cyanobacteria; cyanobactins; bioactive compounds; phylogeny
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MDPI and ACS Style

Martins, J.; Leão, P.N.; Ramos, V.; Vasconcelos, V. N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria. Mar. Drugs 2013, 11, 4902-4916.

AMA Style

Martins J, Leão PN, Ramos V, Vasconcelos V. N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria. Marine Drugs. 2013; 11(12):4902-4916.

Chicago/Turabian Style

Martins, Joana; Leão, Pedro N.; Ramos, Vitor; Vasconcelos, Vitor. 2013. "N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria." Mar. Drugs 11, no. 12: 4902-4916.

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