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N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria
Faculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4169-007, Portugal
CIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, Porto 4050-123, Portugal
* Author to whom correspondence should be addressed.
Received: 13 October 2013; in revised form: 21 November 2013 / Accepted: 21 November 2013 / Published: 9 December 2013
Abstract: Cyanobactins are a recently recognized group of ribosomal cyclic peptides produced by cyanobacteria, which have been studied because of their interesting biological activities. Here, we have used a PCR-based approach to detect the N-terminal protease (A) gene from cyanobactin synthetase gene clusters, in a set of diverse cyanobacteria from our culture collection (Laboratory of Ecotoxicology, Genomics and Evolution (LEGE) CC). Homologues of this gene were found in Microcystis and Rivularia strains, and for the first time in Cuspidothrix, Phormidium and Sphaerospermopsis strains. Phylogenetic relationships inferred from available A-gene sequences, including those obtained in this work, revealed two new groups of phylotypes, harboring Phormidium, Sphaerospermopsis and Rivularia LEGE isolates. Thus, this study shows that, using underexplored cyanobacterial strains, it is still possible to expand the known genetic diversity of genes involved in cyanobactin biosynthesis.
Keywords: cyanobacteria; cyanobactins; bioactive compounds; phylogeny
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Martins, J.; Leão, P.N.; Ramos, V.; Vasconcelos, V. N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria. Mar. Drugs 2013, 11, 4902-4916.
Martins J, Leão PN, Ramos V, Vasconcelos V. N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria. Marine Drugs. 2013; 11(12):4902-4916.
Martins, Joana; Leão, Pedro N.; Ramos, Vitor; Vasconcelos, Vitor. 2013. "N-Terminal Protease Gene Phylogeny Reveals the Potential for Novel Cyanobactin Diversity in Cyanobacteria." Mar. Drugs 11, no. 12: 4902-4916.