Mar. Drugs 2013, 11(11), 4176-4192; doi:10.3390/md11114176
Article

Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae)

1,†email, 2,†email, 1,2email, 1email, 1email, 1email, 1email, 3email, 4email, 4email, 1email, 1email and 1,2,* email
Received: 2 September 2013; in revised form: 18 September 2013 / Accepted: 30 September 2013 / Published: 29 October 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.
Keywords: antiviral activity; HSV-1; marine sponge; Petromica citrina; sulfate sterols
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MDPI and ACS Style

da Rosa Guimarães, T.; Quiroz, C.G.; Rigotto, C.; de Oliveira, S.Q.; de Almeida, M.T.R.; Bianco, É.M.; Moritz, M.I.G.; Carraro, J.L.; Palermo, J.A.; Cabrera, G.; Schenkel, E.P.; Reginatto, F.H.; Simões, C.M.O. Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae). Mar. Drugs 2013, 11, 4176-4192.

AMA Style

da Rosa Guimarães T, Quiroz CG, Rigotto C, de Oliveira SQ, de Almeida MTR, Bianco ÉM, Moritz MIG, Carraro JL, Palermo JA, Cabrera G, Schenkel EP, Reginatto FH, Simões CMO. Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae). Marine Drugs. 2013; 11(11):4176-4192.

Chicago/Turabian Style

da Rosa Guimarães, Tatiana; Quiroz, Carlos G.; Rigotto, Caroline; de Oliveira, Simone Q.; de Almeida, Maria T.R.; Bianco, Éverson M.; Moritz, Maria I.G.; Carraro, João L.; Palermo, Jorge A.; Cabrera, Gabriela; Schenkel, Eloir P.; Reginatto, Flávio H.; Simões, Cláudia M.O. 2013. "Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae)." Mar. Drugs 11, no. 11: 4176-4192.


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