Mar. Drugs 2013, 11(10), 3875-3890; doi:10.3390/md11103875
Article

Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes

1,2email, 3email, 3email, 3email, 3email, 3email, 4,* email, 2email and 2,* email
Received: 4 September 2013; in revised form: 26 September 2013 / Accepted: 27 September 2013 / Published: 14 October 2013
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
View Full-Text   |   Download PDF [509 KB, uploaded 14 October 2013]
Abstract: Mangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produce halogenated metabolites. The FADH2-dependent halogenase genes, identified in PCR-screening, were clustered in distinct clades in the phylogenetic analysis. The coexistence of either polyketide synthase (PKS) or nonribosomal peptide synthetase (NRPS) as the backbone synthetases in the strains harboring the halogenase indicated that these strains had the potential to produce structurally diversified antibiotics. As a validation, a new enduracidin producer, Streptomyces atrovirens MGR140, was identified and confirmed by gene disruption and HPLC analysis. Moreover, a putative ansamycin biosynthesis gene cluster was detected in Streptomyces albogriseolus MGR072. Our results highlight that combined genome mining is an efficient technique to tap promising sources of halogenated natural products synthesized by mangrove-derived actinomycetes.
Keywords: mangrove-derived actinomycetes; genome mining; halogenase; enduracidin; ansamycin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Li, X.-G.; Tang, X.-M.; Xiao, J.; Ma, G.-H.; Xu, L.; Xie, S.-J.; Xu, M.-J.; Xiao, X.; Xu, J. Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes. Mar. Drugs 2013, 11, 3875-3890.

AMA Style

Li X-G, Tang X-M, Xiao J, Ma G-H, Xu L, Xie S-J, Xu M-J, Xiao X, Xu J. Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes. Marine Drugs. 2013; 11(10):3875-3890.

Chicago/Turabian Style

Li, Xue-Gong; Tang, Xiao-Min; Xiao, Jing; Ma, Guang-Hui; Xu, Li; Xie, Shu-Jie; Xu, Min-Juan; Xiao, Xiang; Xu, Jun. 2013. "Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes." Mar. Drugs 11, no. 10: 3875-3890.


Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert