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Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China
* Authors to whom correspondence should be addressed.
Received: 4 September 2013; in revised form: 26 September 2013 / Accepted: 27 September 2013 / Published: 14 October 2013
Abstract: Mangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produce halogenated metabolites. The FADH2-dependent halogenase genes, identified in PCR-screening, were clustered in distinct clades in the phylogenetic analysis. The coexistence of either polyketide synthase (PKS) or nonribosomal peptide synthetase (NRPS) as the backbone synthetases in the strains harboring the halogenase indicated that these strains had the potential to produce structurally diversified antibiotics. As a validation, a new enduracidin producer, Streptomyces atrovirens MGR140, was identified and confirmed by gene disruption and HPLC analysis. Moreover, a putative ansamycin biosynthesis gene cluster was detected in Streptomyces albogriseolus MGR072. Our results highlight that combined genome mining is an efficient technique to tap promising sources of halogenated natural products synthesized by mangrove-derived actinomycetes.
Keywords: mangrove-derived actinomycetes; genome mining; halogenase; enduracidin; ansamycin
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MDPI and ACS Style
Li, X.-G.; Tang, X.-M.; Xiao, J.; Ma, G.-H.; Xu, L.; Xie, S.-J.; Xu, M.-J.; Xiao, X.; Xu, J. Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes. Mar. Drugs 2013, 11, 3875-3890.
Li X-G, Tang X-M, Xiao J, Ma G-H, Xu L, Xie S-J, Xu M-J, Xiao X, Xu J. Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes. Marine Drugs. 2013; 11(10):3875-3890.
Li, Xue-Gong; Tang, Xiao-Min; Xiao, Jing; Ma, Guang-Hui; Xu, Li; Xie, Shu-Jie; Xu, Min-Juan; Xiao, Xiang; Xu, Jun. 2013. "Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes." Mar. Drugs 11, no. 10: 3875-3890.