Next Article in Journal
Efficient Heterologous Transformation of Chlamydomonas reinhardtii npq2 Mutant with the Zeaxanthin Epoxidase Gene Isolated and Characterized from Chlorella zofingiensis
Previous Article in Journal
Polyunsaturated Fatty Acids of Marine Macroalgae: Potential for Nutritional and Pharmaceutical Applications
Mar. Drugs 2012, 10(9), 1936-1954; doi:10.3390/md10091936
Article

Characterization of Isoforms of the Lectin Isolated from the Red Algae Bryothamnion seaforthii and Its Pro-Healing Effect

1
,
2
,
3
,
1
,
1
,
2
,
2
,
3
,
Jr. 4
,
4
,
2
,
3
,
1,*  and 3,*
Received: 19 July 2012 / Revised: 1 September 2012 / Accepted: 1 September 2012 / Published: 4 September 2012
View Full-Text   |   Download PDF [3836 KB, uploaded 24 February 2015]   |   Browse Figures

Abstract

Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.
Keywords: lectins; algal proteins; wound healing lectins; algal proteins; wound healing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Gonzaga do Nascimento-Neto, L.; Carneiro, R.F.; da Silva, S.R.; da Silva, B.R.; Arruda, F.V.S.; Carneiro, V.A.; do Nascimento, K.S.; Saker-Sampaio, S.; da Silva, V.A., Jr.; Porto, A.L.F.; Cavada, B.S.; Sampaio, A.H.; Teixeira, E.H.; Nagano, C.S. Characterization of Isoforms of the Lectin Isolated from the Red Algae Bryothamnion seaforthii and Its Pro-Healing Effect. Mar. Drugs 2012, 10, 1936-1954.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert