Previous studies on the chemical constituents of octocorals belonging to genus Menella (Figure 1) have afforded a series of interesting secondary metabolites [6,7,8,9,10,11,12,13,14,15,16,17], including sesquiterpenoids, menelloides A–D [14,15]. Menelloide E (1) was isolated as a colorless oil (Figure 1), and the molecular formula for this compound was determined to be C₁₅H₁₈O₄ (seven units of unsaturation) using HRESIMS (C₁₅H₁₈O₄ + Na, m/z 285.1105, calculated 285.1103). Comparison of the ¹³C NMR and DEPT data with the molecular formula indicated that there were two exchangeable protons, which required the presence of two hydroxy groups. This deduction was supported by a broad absorption in the IR spectrum at 3413 cm⁻¹. The IR spectrum also showed a strong band at 1746 cm⁻¹, consistent with the presence of an ester group. The ¹³C NMR data for 1 confirmed the presence of 15 carbon signals (Table 1), characterized by DEPT as a methyl, four sp³ methylenes, an sp² methylene, two sp³ methines, an sp² methine, an sp³ quaternary carbon and five sp² quaternary carbons. A suite of resonances at δ_C 175.1 (C-12), 149.8 (C-7), 127.4 (C-11), 102.7 (C-8) and 8.7 (CH₃-13), could be assigned to the α-methyl-α,β-unsaturated-γ-lactone moiety. Two additional unsaturated functionalities were indicated by ¹³C resonances at δ_C 154.5 (C-10), 111.4 (CH-9), 157.6 (C-4) and 104.5 (CH₂-14), suggesting the presence of a trisubstituted olefin and an exocyclic carbon-carbon double bond. Thus, from the reported data, the proposed skeleton of 1 was suggested to be a sesquiterpenoid with three rings.

From the ¹H–¹H COSY spectrum of 1 (Figure 2), it was possible to differentiate between the separate spin systems of H-1/H₂-2/H₂-3, H₂-3/H₂-14 (by allylic coupling), H-1/H-5/H₂-6 and OH-15/H₂-15. These data, together with the HMBC correlations between H-14a/C-3, C-5; H-14b/C-3; H-6β/C-7, C-11; H-9/C-1, C-7, C-10, C-15; and H₃-13/C-7, C-11 (Figure 2), permitted elucidation of the carbon skeleton. The exocyclic carbon-carbon double bond at C-4 was confirmed by the HMBC correlations between H-14a/C-3, C-5 and H-14b/C-3; and further supported by the allylic couplings between H₂-3 and H₂-14. The methylene unit at δ_C 70.2 was correlated to the methylene protons at δ_H 3.67 and 3.61 in the HMQC spectrum and the H-9 olefinic proton signal was ³J-correlated with C-15 (δ_C 70.2), proving the attachment of a hydroxymethyl group at C-10. Thus, the remaining hydroxy group should be positioned at C-8 and concluded to be a part of a hemiketal constellation on the basis of a characteristic carbon signal at δ_C 102.7 (C).

The relative configuration of 1 was elucidated mainly from a NOESY spectrum as being compatible with that of 1 offered by computer modeling (Figure 3), in which the close contacts of atoms in space calculated were consistent with the NOESY correlations [18]. In the NOESY experiment of 1, H-5 exhibited a correlation with H-1, indicating that these two protons (H-1 and H-5) were situated on the same face and assigned as β protons. One proton of C-6 methylene (δ_H 3.51) exhibited correlations with H-1 and OH-8 (δ_H 2.49), but not with H₃-13, indicating that this proton and the hydroxy group at C-8 were β-oriented in 1 by molecular modeling analysis (Figure 3). This observation was supported by a correlation between H-6α (δ_H 3.00) and H₃-13, but not with OH-8, although a correlation was also found between H-5 and H-6α. The Z-configuration of the C-7/11 double bond was elucidated from a correlation between H-6α and the C-13 vinyl methyl. Correlations observed between H-9 and H-15a/b reflected the E geometry of the double bond at C-9/10. From the above evidence, the relative configurations of the chiral carbons of 1 were assumed to be 1S*, 5S* and 8S*. On the basis of the above analysis, the relative structure of 1 was determined.

The octocoral L. crassum (Figure 4), distributed in the waters of Taiwan has afforded various new cembranoids [19,20,21,22,23,24,25,26], including lobocrassins A–E [24]; new glycolipids [27]; and new α-tocopherols [28]. The molecular formula for lobocrassin F (2) (Figure 4) was determined to be C₂₀H₃₀O₂ (six units of unsaturation) using HRESIMS (C₂₀H₃₀O₂ + Na, m/z 325.2145, calculated 325.2143). Comparison of the ¹³C NMR and DEPT data with the molecular formula indicated that there was an exchangeable proton, which required the presence of a hydroxy group. This deduction was supported by a broad absorption in the IR spectrum at 3265 cm⁻¹. The ¹³C NMR data for 1 confirmed the presence of twenty carbon signals (Table 2), characterized by DEPT as four methyls, six sp³ methylenes, an sp² methylene, an sp³ methine, two sp² methines, an sp³ quaternary carbons and five sp² quaternary carbons. Compound 2 was determined to possess a tetrasubstituted olefin (δ_C 108.2, C-1; 144.2, C-14), two trisubstituted olefins (δ_C 131.5, C-12; 130.0, C-8; 127.8, CH-7; 127.7, CH-11) and an exocyclic carbon-carbon double bond (δ_C 144.3, C-15; 113.7, CH₂-17). The above functionalities accounted for four of the seven degrees of unsaturation, suggesting a bicyclic structure of 2.

From the ¹H–¹H COSY spectrum of 2 (Figure 5), it was possible to identify the separate spin systems of H₂-2/H-3, H₂-5/H₂-6/H-7, H₂-9/H₂-10/H-11 and H₃-16/H₂-17 (by allylic coupling). These data, together with the HMBC correlations between protons and quaternary carbons of 1, such as H₂-2, H₂-13, H₃-16, H₂-17/C-1; H₂-2, H₂-5, H₂-6, H₃-18/C-4; H₂-6, H₂-9, H₂-10, H₃-19/C-8; H₂-10, H₂-13, H₃-20/C-12; H₂-2, H₂-13/C-14; and H₂-2, H₃-16/C-15 (Figure 5), permitted elucidation of the carbon skeleton. The vinyl methyls attached at C-8, C-12 and C-15 were confirmed by the HMBC correlations between H₃-19/C-7, C-8, C-9; H₃-20/C-11, C-12, C-13; and H₃-16/C-1, C-15, C-17 (Figure 5). However, because no HMBC correlation was observed between H-3 and C-14, the remaining hydroxy group was positioned at C-3, and an ether bridge was determined to be located between C-4 and C-14 to form a pyran ring. In order to confirm this ratiocination, acetylation of 2 was performed and its derivative 3-O-acetyllobocrassin F (3) (Figure 4) was obtained in high yield (95%). This result indicates that a secondary hydroxy group attached at C-3 in 2 is necessary.

The relative configuration of 2 was elucidated mainly from a NOESY spectrum as being compatible with that of 2 offered by computer modeling (Figure 6), in which the close contacts of atoms in space calculated were consistent with the NOESY correlations [18]. In the NOESY experiment for 2, correlations were observed between H-3 and H₃-18, suggesting that these protons are located on the same face and can be assigned as α protons. Correlations observed between H-7/H₂-9 and H-11/H₂-13, as well as the lack of correlation between H-7/H₃-19 and H-11/H₃-20, reflected the E geometry of the double bonds at C-7/8 and C-11/12. Additionally, H-17a (δ_H 4.72) was correlated with H-13α (δ_H 2.98), suggesting an s-cis diene moiety in 2. On the basis of the above findings, the structure of 2 was elucidated and the chiral carbons of 2 were assigned as 3S* and 4S*.

In previous studies, cembranoids possessing an ether linkage to form a pyran or a tetrahydropyran rings have rarely found [29,30,31,32]. To the best of our knowledge, lobocrassin F (2) is the first pyrancembranoid possessing a C-4/14 ether linkage to be obtained from soft corals belonging to the genus Lobophytum.

The in vitro anti-inflammatory effects of terpenoids 1 and 2 were tested (Table 3). Diterpenoid 2 (lobocrassin F) was found to show a significant inhibitory effect on the release of elastase by human neutrophils.

Optical rotations were measured on a Jasco P-1010 digital polarimeter. Infrared spectra were recorded on a Varian Diglab FTS 1000 FT-IR spectrometer; peaks are reported in cm⁻¹. NMR spectra were recorded on Varian Inova 500 or Varian Mercury Plus 400 NMR spectrometers using the residual CHCl₃ signal (δ_H 7.26 ppm) as the internal standard for ¹H NMR and CDCl₃ (δ_C 77.1 ppm) for ¹³C NMR. Coupling constants (J) are given in Hz. ESIMS and HRESIMS were recorded using a Bruker APEX II mass spectrometer. Column chromatography was performed on silica gel (230–400 mesh, Merck, Darmstadt, Germany). TLC was carried out on precoated Kieselgel 60 F₂₅₄ (0.25 mm, Merck); spots were visualized by spraying with 10% H₂SO₄ solution followed by heating. HPLC was performed using a system comprised of a Hitachi L-7100 pump, a Hitahci L-7455 photodiode array detector and a Rheodyne injection port. A normal phase column (Hibar 250 × 10 mm, Merck, silica gel 60, 5 µm) was used for HPLC.

Implementation of the MM2 force field [18] in CHEM3D PRO software from CambridgeSoft Corporation (Cambridge, MA, USA; ver. 9.0, 2005) was used to calculate molecular models.

Human neutrophils were obtained by means of dextran sedimentation and Ficoll centrifugation. Measurements of superoxide anion generation and elastase release were carried out according to previously described procedures [33,34]. Briefly, superoxide anion production was assayed by monitoring the superoxide dismutase-inhibitable reduction of ferricytochrome c. Elastase release experiments were performed using MeO-Suc-Ala-Ala-Pro-Valp-nitroanilide as the elastase substrate.