Mar. Drugs 2012, 10(11), 2596-2607; doi:10.3390/md10112596
Article

Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus

1 Institute of Oceanology, Chinese Academy of Science, 7 Nanhai Rd, Qingdao 266071, China 2 Key Laboratory for Rare Diseases of Shandong Province, Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan 250062, China
* Author to whom correspondence should be addressed.
Received: 22 August 2012; in revised form: 25 September 2012 / Accepted: 19 October 2012 / Published: 21 November 2012
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Abstract: Mere15 is a novel polypeptide from Meretrix meretrix Linnaeus with cytotoxicity in solid cancer cells. In this study, we investigated its activity on human K562 chronic myelogenous leukemia cells. Mere15 inhibited the growth of K562 cells with IC50 values of 38.2 μg/mL. Mere15 also caused concentration dependent induction of apoptosis, with overproduction of reactive oxygen species and loss of mitochondrial membrane potential. Moreover, Mere15 arrested cell cycle progression at G0/G1 phase of K562 cells in a concentration dependent manner. In addition, Mere15 caused the disassembly of the microtubule cytoskeleton in K562 cells and inhibited the polymerization of tubulin in a cell free system via interaction with tubulin. We concluded that Mere15 was cytotoxic to K562 leukemia cells and the cytotoxicity was related to the apoptosis induction, cell cycle arrest and microtubule disassembly. These results implied that Merer15 was a broad spectrum anticancer polypeptide, not only cytotoxic to various solid cancer cells but also to the chronic myelogenous leukemia cells. Mere15 may have therapeutic potential for the treatment of leukemia.
Keywords: Meretrix meretrix Linnaeus; Mere15; apoptosis; anticancer

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MDPI and ACS Style

Liu, M.; Zhao, X.; Zhao, J.; Xiao, L.; Liu, H.; Wang, C.; Cheng, L.; Wu, N.; Lin, X. Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus. Mar. Drugs 2012, 10, 2596-2607.

AMA Style

Liu M, Zhao X, Zhao J, Xiao L, Liu H, Wang C, Cheng L, Wu N, Lin X. Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus. Marine Drugs. 2012; 10(11):2596-2607.

Chicago/Turabian Style

Liu, Ming; Zhao, Xiangzhong; Zhao, Jin; Xiao, Lin; Liu, Haizhou; Wang, Cuicui; Cheng, Linyou; Wu, Ning; Lin, Xiukun. 2012. "Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus." Mar. Drugs 10, no. 11: 2596-2607.

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