Mar. Drugs 2012, 10(11), 2519-2534; doi:10.3390/md10112519
Article

Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella

Jean-Luc Rolland 1,* email, Kevin Pelletier 1 email, Estelle Masseret 1 email, Fabien Rieuvilleneuve 1 email, Veronique Savar 2 email, Adrien Santini 1 email, Zouher Amzil 2 email and Mohamed Laabir 1 email
1 IFREMER, Université Montpellier 2, Centre National de la Recherche Scientifique, IRD, UM1, UMR 5119 “Ecologie des Systèmes Marins Côtiers”, Place E. Bataillon, CC93, 34095 Montpellier cedex 5, France 2 IFREMER, Département Environnement, Microbiologie et Phycotoxines (EMP/PHYC), Rue de l’Ile d’Yeu BP 21105 44311 Nantes CEDEX 3, France
* Author to whom correspondence should be addressed.
Received: 20 September 2012; in revised form: 16 October 2012 / Accepted: 26 October 2012 / Published: 12 November 2012
(This article belongs to the Special Issue Marine Neurotoxins)
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Abstract: The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two α-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The α-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins.
Keywords: Crassostrea gigas; Alexandrium catenella; PSP toxins; digestion; gene expression

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MDPI and ACS Style

Rolland, J.-L.; Pelletier, K.; Masseret, E.; Rieuvilleneuve, F.; Savar, V.; Santini, A.; Amzil, Z.; Laabir, M. Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella. Mar. Drugs 2012, 10, 2519-2534.

AMA Style

Rolland J-L, Pelletier K, Masseret E, Rieuvilleneuve F, Savar V, Santini A, Amzil Z, Laabir M. Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella. Marine Drugs. 2012; 10(11):2519-2534.

Chicago/Turabian Style

Rolland, Jean-Luc; Pelletier, Kevin; Masseret, Estelle; Rieuvilleneuve, Fabien; Savar, Veronique; Santini, Adrien; Amzil, Zouher; Laabir, Mohamed. 2012. "Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella." Mar. Drugs 10, no. 11: 2519-2534.

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