Next Article in Journal
Assessment of the Bacteriocinogenic Potential of Marine Bacteria Reveals Lichenicidin Production by Seaweed-Derived Bacillus spp.
Previous Article in Journal
Cytotoxic Activity of Semi-Synthetic Derivatives of Elatol and Isoobtusol
Previous Article in Special Issue
The Transcriptome of Bathymodiolus azoricus Gill Reveals Expression of Genes from Endosymbionts and Free-Living Deep-Sea Bacteria
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2012, 10(10), 2265-2279; doi:10.3390/md10102265

Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones

1
RNA and Transcriptomics Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, N9037 Tromsø, Norway
2
Pharmacology Group, Department of Pharmacy, Faculty of Health Sciences, University of Tromsø, N9037 Tromsø, Norway
3
Marine Genomics Group, Faculty of Biosciences and Aquaculture, University of Nordland, N8049 Bodø, Norway
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 August 2012 / Revised: 8 October 2012 / Accepted: 10 October 2012 / Published: 18 October 2012
(This article belongs to the Special Issue Metagenomics in Biodiscovery from Oceans)
View Full-Text   |   Download PDF [745 KB, uploaded 24 February 2015]   |  

Abstract

Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries. View Full-Text
Keywords: deep sequencing; drug discovery; marine bioprospecting; neurotoxin; sea anemone; transcriptomics deep sequencing; drug discovery; marine bioprospecting; neurotoxin; sea anemone; transcriptomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Urbarova, I.; Karlsen, B.O.; Okkenhaug, S.; Seternes, O.M.; Johansen, S.D.; Emblem, Å. Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones. Mar. Drugs 2012, 10, 2265-2279.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top