Next Article in Journal
Involvement of HDAC1 and HDAC3 in the Pathology of Polyglutamine Disorders: Therapeutic Implications for Selective HDAC1/HDAC3 Inhibitors
Next Article in Special Issue
A Bombesin-Shepherdin Radioconjugate Designed for Combined Extra- and Intracellular Targeting
Previous Article in Journal
Synthesis, Screening and Pharmacokinetic Evaluation of Potential Prodrugs of Bupropion. Part One: In Vitro Development
Previous Article in Special Issue
Synthesis, Radiolabelling and In Vitro Characterization of the Gallium-68-, Yttrium-90- and Lutetium-177-Labelled PSMA Ligand, CHX-A''-DTPA-DUPA-Pep
Article Menu

Export Article

Open AccessArticle
Pharmaceuticals 2014, 7(5), 621-633; doi:10.3390/ph7050621

Development and Successful Validation of Simple and Fast TLC Spot Tests for Determination of Kryptofix® 2.2.2 and Tetrabutylammonium in 18F-Labeled Radiopharmaceuticals

1
Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tübingen, Röntgenweg 15, Tübingen D-72076, Germany
2
Department of Radiochemistry, ABX GmbH, Heinrich-Gläser-Straße 10, Radeberg D-01454, Germany
*
Author to whom correspondence should be addressed.
Received: 12 February 2014 / Revised: 1 May 2014 / Accepted: 7 May 2014 / Published: 14 May 2014
(This article belongs to the Special Issue Radiopharmaceutical Chemistry between Imaging and Radioendotherapy)
View Full-Text   |   Download PDF [286 KB, uploaded 14 May 2014]   |  

Abstract

Kryptofix® 2.2.2 (Kry) or tetrabutylammonium (TBA) are commonly used as phase transfer catalysts in 18F-radiopharmaceutical productions for positron emission tomography (PET). Due to their toxicity, quality control has to be performed before administration of the tracer to assure that limit concentration of residual reagent is not reached. Here, we describe the successful development and pharmaceutical validation (for specificity, accuracy and detection limit) of a simplified color spot test on TLC plates. We were able to prove its applicability as a general, time and resources saving, easy to handle and reliable method in daily routine analyzing 18F-tracer formulations for Kry (in [18F]FDG or [18F]FECh) or TBA contaminations (in [18F]FLT) with special regard to complex matrix compositions. View Full-Text
Keywords: Kryptofix® 2.2.2; tetrabutylammonium; fluorine-18; quality control; thin layer chromatography (TLC) spot test Kryptofix® 2.2.2; tetrabutylammonium; fluorine-18; quality control; thin layer chromatography (TLC) spot test
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kuntzsch, M.; Lamparter, D.; Brüggener, N.; Müller, M.; Kienzle, G.J.; Reischl, G. Development and Successful Validation of Simple and Fast TLC Spot Tests for Determination of Kryptofix® 2.2.2 and Tetrabutylammonium in 18F-Labeled Radiopharmaceuticals. Pharmaceuticals 2014, 7, 621-633.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Pharmaceuticals EISSN 1424-8247 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top