Abstract: Sharpless asymmetric dihydroxylation of styrene derivative 6 afforded chiral triols (R)-7 and (S)-7, which were cyclized with tosyl chloride in the presence of Bu2SnO to provide 2-benzopyrans (R)-4 and (S)-4 with high regioselectivity. The additional hydroxy moiety in the 4-position was exploited for the introduction of various substituents. Williamson ether synthesis and replacement of the Boc protective group with a benzyl moiety led to potent σ1 ligands with high σ1/σ2-selectivity. With exception of the ethoxy derivative 16, the (R)-configured enantiomers represent eutomers with eudismic ratios of up to 29 for the ester (R)-18. The methyl ether (R)-15 represents the most potent σ1 ligand of this series of compounds, with a Ki value of 1.2 nM and an eudismic ratio of 7. Tosylate (R)-21 was used as precursor for the radiosynthesis of [18F]-(R)-20, which was available by nucleophilic substitution with K[18F]F K222 carbonate complex. The radiochemical yield of [18F]-(R)-20 was 18%–20%, the radiochemical purity greater than 97% and the specific radioactivity 175–300 GBq/µmol. Although radiometabolites were detected in plasma, urine and liver samples, radiometabolites were not found in brain samples. After 30 min, the uptake of the radiotracer in the brain was 3.4% of injected dose per gram of tissue and could be reduced by coadministration of the σ1 antagonist haloperidol. [18F]-(R)-20 was able to label those regions of the brain, which were reported to have high density of σ1 receptors.
Keywords: 2-benzopyrans; Sharpless Asymmetric Dihydroxylation; spirocycles; σ affinity; radiochemistry; positron emission tomography; autoradiography; organ distribution
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Holl, K.; Schepmann, D.; Fischer, S.; Ludwig, F.-A.; Hiller, A.; Donat, C.K.; Deuther-Conrad, W.; Brust, P.; Wünsch, B. Asymmetric Synthesis of Spirocyclic 2-Benzopyrans for Positron Emission Tomography of σ1 Receptors in the Brain. Pharmaceuticals 2014, 7, 78-112.
Holl K, Schepmann D, Fischer S, Ludwig F-A, Hiller A, Donat CK, Deuther-Conrad W, Brust P, Wünsch B. Asymmetric Synthesis of Spirocyclic 2-Benzopyrans for Positron Emission Tomography of σ1 Receptors in the Brain. Pharmaceuticals. 2014; 7(1):78-112.
Holl, Katharina; Schepmann, Dirk; Fischer, Steffen; Ludwig, Friedrich-Alexander; Hiller, Achim; Donat, Cornelius K.; Deuther-Conrad, Winnie; Brust, Peter; Wünsch, Bernhard. 2014. "Asymmetric Synthesis of Spirocyclic 2-Benzopyrans for Positron Emission Tomography of σ1 Receptors in the Brain." Pharmaceuticals 7, no. 1: 78-112.