- freely available
Pharmaceuticals 2013, 6(7), 867-880; doi:10.3390/ph6070867
Published: 4 July 2013
Abstract: Reviewed here are recent attempts to produce protein-based artificial oxygen carriers (“blood substitutes”). Most of these involve chemical or physical modifications on hemoglobin, although a recent line of research using hemerythrin instead of hemoglobin is also described. The focus is set on the extent to which these modifications alter the redox reactivity of the proteins, and on ways in which this can be done systematically and purposefully, within the framework of a working hypothesis where redox side-reactions hold an important role in the physiological outcome of experimental transfusions with artificial oxygen carriers.
1. Introduction: Structure and Reactivity of Hemoglobin
Hemoglobin (Hb) is the protein responsible for the transport of oxygen from the lungs to the other tissues of the body and participates in the transport of carbon dioxide in the opposite direction. Hb is a metalloprotein whose structure consists of four monomeric units: two α and two β chains, held together by noncovalent interactions - hydrogen bonds and salt bridges. Each monomer contains a heme b group whose iron atom binds molecular oxygen; trans to the oxygen coordination position the iron is bound to the polypeptide via the so-called “proximal histidine” (Figure 1) [1,2].
Transport of oxygen by hemoglobin is achieved by alternating the structure of two forms: the high oxygen affinity relaxed (R) state oxyhemoglobin, vs. the low oxygen affinity tense (T) state deoxyhemoglobin. Allosteric effectors such as 2,3 BPG modulate this reactivity. The negatively charged BPG is inserted into the central cavity between the two β chains of deoxy molecule, next to the positively charged His2, Lys82 and His143 [1,3]. Oxygen binding in hemoglobin is cooperative, with the binding of an O2 molecule facilitating the binding of further oxygen to the remaining subunits. Oxygen binding and conversion to the R state cause conformational changes that involving the rotation of one dimer relative to the other. These changes in turn cause reduction of the space allocated for the BPG binding site, thus forcing it to leave the Hb molecule. In addition to BPG, other factors such as H+, NO, ATP, Cl−, or CO2 modulate the oxygen affinity [3,4]. Bovine Hb (bHb) is not as dependant as human Hb on the effects of BPG, thus providing an advantage towards simplicity for bHb in preparation of blood substitites . Additionally, other sources of Hb are also considered, including more exotic ones such as Lumbricus, arctic fish and others [5,6].
To bind and transport oxygen, the iron must be in the ferrous (Fe2+) state. On the other hand, as a side-reaction, oxyhemoglobin also undergoes spontaneous oxidation (autooxidation) of the iron, forming metHb (Fe3+); this latter state cannot bind oxygen :
Overall, as an effect of the autooxidation reaction reactive oxygen species (ROS) such as hydrogen peroxide and free radicals (especially superoxide, but also hydroxyl radical and hydrogen peroxide generated upon degradation of superoxide) are generated, with damaging effects on organs and living cells. Methemoglobin reductase is the enzyme within the erythrocyte that acts to reduce the met form back to deoxyHb . A separate range of reactions takes Hb onto nitrosative stress routes .
2. Reaction of Peroxide with the Heme Group
The heme group is intrinsically reactive towards hydrogen peroxide, typically engaging in a two-electron reduction of H2O2 to H2O. The reaction of hydrogen peroxide with oxyHb results in the oxidation of the Hb to Fe4+, with two electrons moving from iron to H2O2. The reaction with metHb results in the formation of Fe4+, with a second electron coming from the globin and hence leading to free radical formation (R, where “R” may be an aminoacid from within the hemoglobin or, if available, an external reducing agent such as ascorbate, urate, phenolics, and others) [9,10,11,12,13]:
Hydrogen peroxide thus activates hemoglobin, leading to high valent ferryl (Fe4+-oxo) intermediates at the heme iron—Compound I (ferryl plus radical) and Compound II (ferryl). Ferryl Hb is constantly produced in the body and in condition of stress, illness or high-intensity exercise; it acts as a radical-generating agent, inducing, among others, the peroxidation of lipids .
Cell-free hemoglobin is lethal, because of extravasation, redox reactivity, kidney filtration, oxygen affinity, NO dioxygenase reactivity [14,15,16,17]. However, purified hemoglobin can be a reasonable blood substitute candidate once its reactivities can be controlled and/or reduced. To this end, a series of chemical modifications/transformations were performed as follows/as further described (with main directions summarized in Table 1).
|Table 1. Options for hemoglobin modification.|
|Modified Hb forms|
|Single step derivatization|
|Genetically modify hemoglobin|
Tyr ↔ Val, Trp / Phe ↔Tyr
|[possibly combined with any of the above]|
|The encapsulation of hemoglobin|
4. Single-step Hemoglobin Derivatization
There are some protocols for derivatization of hemoglobin with two N-hydroxysuccinimide esters (NHS-esters): disuccinimidyl suberate (DSS) and (methyl-PEG12)3-PEG4-N-hydroxysuccinimide ester (TMS). They allow protein crosslinking without toxic side-products and forming peptide bond with lysine residue in one single step .
The first agents, DSS (Figure 4), is a non-cleavable membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide ester at each end of an 8-carbon spacer which reacts with lysine residues to form peptide bond in one single step .
DSS may be employed for obtaining Hb polymers, and the increase in autooxidation rate incurred by this polymerization is completely reversed when BSA is co-polymerized with Hb. The copolymers show very little change in autooxidation and oxygen affinity compared to native Hb, and low intensity of free radicals – less than in native Hb .
A third approach towards reducing heomglobin’s pro-oxidant activity has involved derivatization of the protein surface with polymeric systems. The polymers have the requirement to be non-toxic, non-immunogenic, non-antigenic, and soluble in water; such are polyethylene glycol, and some oligo- and polysaccharides. This method has similar results to those based on polymerization, as the aim is again to increase molecular weight, reduce toxicity, and prevent renal excretion.
Typically, the first step in pegylation is suitable derivatization of the PEG at one or both ends, based on the type of available reactive group on protein. The aminoacids most frequently involved in pegylation are lysine and cysteine . If only one end of the PEG is reactive, the Hb molecule is simply decorated with PEG strands - but there are several possibilities of intra- and intermolecular cross-linking if both ends of the reagent are functional. In the second case, a large part of research was focused of the pyridoxalated Hb where, contrary to glutaraldehyde-polymerized pyridoxalated Hb, cooperativity was conserved. In both cases the size of the protein is increased. [15,30,31].
TMS (Figure 5), is a branched amine-reactive PEGylation reagent (methyl-PEG12)3-PEG4-N-hydroxysuccinimide ester. Each methyl-terminated PEG (mPEG) branch contains 12 ethylene glycol units. The three branches are attached to a 4-unit PEG stem that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at the distal end .
Our published results show that rate of autooxidation during the process of pegylation with TMS is very low, and that the dioxygen affinity of this derivatized Hb is very close to that of native bovine Hb, with the particular observation that, unlike with most other derivatization protocols . In cell culture tests (human umbilical vein epithelial cells, HUVEC), the derivatization protocol induces no toxic effect. These results show promise towards applicability for production of hemoglobin-based blood substitutes .
A fourth approach has been to genetically modify hemoglobin. Genetic engineering offers an alternative to chemically modified Hb. Modifications have been made to increase the tetramer’s stability, decreased its oxygen affinity and to reduce the reactivity towards NO. The latter inconvenient can be dealt with by modifying amino acids gating access of small molecules towards the heme site [8,15,16,32]. Examples of recombinant hemoglobin include rHb1.0, rHb1.1, rHb1.2—proteins that contain the Presbyterian mutation and have zero, one or two glycines linking the alpha subunits, respectively .
Recombinant cross-linked Hb (rHb1.1, Optro) is a variant of human Hb, expressed in E. coli and developed for commercialization. This hemoglobin contains two modification: the first modification fuses the alpha subunits: the C-terminal arginine of one α chain and the N-terminal valine of the other are genetically linked by a glycine bridge . The other modification contains the Presbyterian mutation and involves the change of Asn108 →Lys in both β-chains. This mutation decreases the oxygen affinity to a level comparable to that of native one [33,34].
Hemoglobin Polytaur is an autopolymerizing human-bovine hybrid mutant and was obtained by polymerization of Hb Minotaur—a hybrid that contains α-human and β-bovine subunits . The reaction of polymerization occurs via the oxidation of thiol groups (-R-SH SH-R)→ -R-S-S-R) between Cys residues introduced at the external position β9. Two forms were obtained: Hb Polytaur (Cys104α→Ser, Cys93β→Ala) with MW: 500 kDa and (Hb Polytaur)n with MW 1,000 kDa or higher. The polymer designated as Hb Polytaur has an oxygen affinity similar with Hb-A but the rate of autooxidation is increases [15,35,36].
The encapsulation of hemoglobin in liposomes or vesicles is another approach that can influence hemoglobin’s reactivity . The encapsulation of natural or modified Hb in an artificial membrane is expected to lead to fewer side effects, longer intravascular duration and greater oxygen-carrying capacity .
Liposomes are closed vesicles of lipid-bilayer membrane and are the most investigated system because of their higher biocompatibility and biodegradability and their capacity to increase the retention time of entrapped agents inside the cell . The lipid composition of such vesicles is based on a mixture of saturated phospholipids with a large number of carbon atoms like distearoylphosphatidylcholine or dipalmitoylphosphatidylcholine and cholesterol, which reduces membrane permeability prevents hemoglobin oxidation and denaturation, and reduces lipid peroxidation [14,22]. The size of liposome and the encapsulation efficiency are decisive factors regarding their use. Larger sized liposomes are rapidly removed from circulation by reticuloendothelial system (RES). Another disadvantage is the extensive oxidation of the protein during encapsulation and storage. In this case it is essential to co-encapsulate antioxidants such as catalase, methemoglobin reductase, superoxide dismutase, and glutathione. The oxygen affinity can also be modified to be close to that of RBCs, by co-encapsulation of allosteric effectors such as 2,3-BPG, inositolphosphate or pyridoxal phosphate. Co-encapsulation with the latter effector generates a product with an oxygen affinity similar to the red blood cell [22,37,38,39]. Liposome-encapsulated hemoglobin (LEH) has been modified to achieve stability in circulating blood. Thus, derivatives of polyethylene glycol, polysaccharides and phosphatidylinositol were added to this product in order to modify the surface of liposomes and to thereby decrease reticuloendothelial system uptake and prolong LEH circulation persistence. Surface modification with PEG appeared to be the most promising strategy for increasing the circulation time (65 h). The stability of the bilayer membranes was also increased by polymerization, with a 4-fold increase in plasma (Figure 6) [16,40].
Liposome-encapsulated Hb was first produced in the United States. Later it became the subject and the target of Japanese researchers, who have dubbed the term hemoglobin vesicles (HbV). As in the case of liposomes, the Hb solution is encapsulated within a phospholipid bilayer membrane. A particle has a diameter of ~250 nm and contains about 30,000 Hb molecules. One of the properties that can influence the phospholipid bilayer is the fluidity and temperature . Thus, the number of bilayers decreases when the surface potential of the lipid membrane becomes more negative and membrane fluidity is reduced inversely with the temperature. The encapsulation efficiency, oxygen affinity and the rate of metHb formation are sensitive to pH, the optimum being 7 to 7.4 .
A sixth approach involves supplementation with small-molecule antioxidants (e.g., ascorbate or selenium). The aim of this method is to provide an oxygen carrier with antioxidant properties. In order to reduce the autooxidation rate and prooxidant reactivity, various antioxidants can be introduced into the reaction mixture when derivatizing hemoglobin, under conditions where the dioxygen affinity and Hill coefficient are maintained at levels close to that of native hemoglobin (e.g., as seen in Table 2) . These features are particularly useful since chemical derivatization of hemoglobin, especially in the form of crosslinking, can lead to autooxidation, increased affinity, and decreased cooperativity. Also, copolymerization with BSA lowers the amount of free radicals generated upon reaction of hemoglobin with hydrogen peroxid. This may be expected to be a positive feature in a blood substitute [25,41].
|Table 2. Autooxidation rates and P50 values expressed in percentage increases relative to nHb, and Hill coefficients for Hb-albumin copolymers .|
|Hb-oATP-1 mM BSA||−33%||−55%||1.97|
|Hb-oATP-2 mM BSA||−33%||−36%||1.79|
5. Hemerythrin-Based Blood Substitutes
Hemerythrin-based blood substitutes use as active ingredient a protein responsible for oxygen transport in the marine invertebrates that employs a non-heme diiron active site (Fe(II)-Fe(II)). Hemerythrin (Hr) was shown to avoid reactivity towards hydrogen peroxide, nitric oxide and nitrite. Another advantage is the higher molecular weight than Hb (108 kDa vs 64 kDa), which should lead to lower levels of extravasation or elimination through the kidney [42,43]. Until now, glutaraldehyde-polymerized hemerythrin and polyethylene glycol-derivatized hemerythrin were successfully obtained. The effects of these chemical modifications on molecular weight, autooxidation rate and oxygen affinity appear to be favorable for blood substitute applications [42,43,44,45].
The toxicity of hemerythrin and of its chemical derivates was tested on cultures of human cells, comparing their performance with that of the representative competitor, glutaraldehyde-polymerized bovine hemoglobin . Hemerythrin (native or derivatized) exhibits a proliferative effect on HUVEC cultures, as opposed to a slight inhibitory effect of Hb. A similar positive effect is displayed on human lymphocytes by glutaraldehyde-polymerized hemerythrin, but not by native or polyethylene glycol-derivatized hemerythrin. Thus, hemerythrin and its chemical derivatives were found to be less toxic than native Hb and glutaraldehyde-polymerized Hb .
To conclude, there is now available a library of potential blood substitutes with varying degrees of reactivity towards oxidative stress agents. For several of these, there appears to be some degree of positive correlation between lack of reactivity in chemical versus cell culture tests. Ongoing tests on animal systems are hoped to confirm that rational design of a protein-based blood substitute is within reach.
Funding from the Romanian Ministry of Education and Research, (grants PN II 312 and 140/2008) is gratefully acknowledged.
Conflict of Interest
The authors declare no conflict of interest.
- Antonini, E.; Brunori, M. Hemoglobin and Myoglobin in Their Reaction with Ligands; North-Holland Publishing Company: Amsterdam, Netherlands, 1971.
- Perutz, M.F. Mechanisms of cooperativity and allosteric regulation in proteins. Q. Rev. Biophys. 1989, 22, 139–237, doi:10.1017/S0033583500003826.
- Kinoshita, A.; Tsukada, K.; Soga, T.; Hishiki, T.; Ueno, Y.; Nakayama, Y.; Tomita, M.; Suematsu, M. Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes: Simulation and its verification by metabolome analysis. J. Biol. Chem. 2007, 282, 10731–10741.
- Nelson, D.L.; Cox, M.M. Lehninger Principles of Biochemistry, 4th ed.; W.H. Freeman & Company: New York, NY, USA, 2004.
- Harrington, J.P.; Orlik, K.; Zito, S.L.; Wollocko, J.; Wollocko, H. Structural and redox behavior of OxyVita, a zero-linked polymeric hemoglobin: Comparison with natural acellular polymeric hemoglobins. Artif. Cells Blood Substit. Immobil. Biotechnol. 2010, 38, 64–68, doi:10.3109/10731191003634562.
- Buehler, P.W.; Alayash, A.I. All hemoglobin-based oxygen carriers are not created equally. Biochim. Biophys. Acta 2008, 1784, 1378–1381, doi:10.1016/j.bbapap.2007.12.009.
- Shikama, K. Nature of the FeO2 bonding in myoglobin and hemoglobin: A new molecular paradigm. Prog. Biophys. Mol. Biol. 2006, 91, 83–162, doi:10.1016/j.pbiomolbio.2005.04.001.
- Alayash, A.I. Oxygen therapeutics: Can we tame haemoglobin? Nat. Rev. Drug Discov. 2004, 3, 152–159, doi:10.1038/nrd1307.
- Svistunenko, D.A. Reaction of haem containing proteins and enzymes with hydroperoxides: The radical view. Biochim. Biophys. Acta 2005, 1707, 127–155, doi:10.1016/j.bbabio.2005.01.004.
- Alayash, A.I.; Patel, R.P.; Cashon, R.E. Redox reactions of hemoglobin and myoglobin: Biological and toxicological implications. Antiox. Redox Signal. 2001, 3, 313–327, doi:10.1089/152308601300185250.
- Kanner, J.; German, J.B.; Kinsellas, J.E. Initiation of lipid peroxidation in biological systems. Crit. Rev. Food Sci. Nutr. 1987, 25, 317–364.
- Silaghi-Dumitrescu, R.; Reeder, B.J.; Nicholls, P.; Cooper, C.E.; Wilson, M.T. Ferryl haem protonation gates peroxidatic reactivity in globins. Biochem. J. 2007, 403, 391–395.
- Simoni, J.; Villanueva-Meyer, J.; Simoni, G.; Moeller, J.F.; Wesson, D.E. Control of oxidative reaction of hemoglobin in the design of blood substitutes: Role of the ascorbate-glutathione antioxidant system. Artif. Organs 2009, 33, 115–126, doi:10.1111/j.1525-1594.2008.00695.x.
- Chang, T.M.S. Blood Substitutes and Oxygen Carriers; Marcel Dekker: New York, NY, USA, 1992.
- Riess, J.G. Oxygen carriers (“blood substitutes”)-raison d’etre, chemistry, and some physiology. Chem. Rev. 2001, 101, 2797–2919, doi:10.1021/cr970143c.
- Winslow, R.M. Blood substitutes; Elsevier Academic Press: London, UK, 2006.
- Simoni, J.; Simoni, G.; Moeller, J.F. Intrinsic toxicity of hemoglobin: How to counteract it. Artif. Organs 2009, 33, 100–109, doi:10.1111/j.1525-1594.2008.00693.x.
- Dunne, J.; Caron, A.; Menu, P.; Alayash, A.; Buehler, P.W.; Wilson, M.T.; Silaghi-Dumitrescu, R.; Faivre, B.; Cooper, C.E. Ascorbate removes key precursors to oxidative damage by cell-free haemoglobin in vitro and in vivo. Biochem. J. 2006, 399, 513–524.
- Deac, F.; Todea, A.; Silaghi-Dumitrescu, R. Glutaraldehyde derivatization of hemoglobin: A potential blood substitute. In Metal Elements in Environment; Cluj University Press: Cluj-Napoca, Romania, 2009; pp. 165–173.
- Chang, W.H.; Chang, Y.; Chen, Y.C; Sung, H.W. Hemoglobin polymerized with a naturally occuring crosslinking agent as a blood substitute: In vitro and in vivo studies. Artif. Cells Blood Substit Immobil. Biotechnol. 2004, 32, 243–262.
- Fischer-Fodor, E.; Mot, A.; Deac, F.; Arkosi, M.; Silaghi-Dumitrescu, R. Towards hemerythrin-based blood substitutes: Comparative performance to hemoglobin on human leukocytes and umbilical vein endothelial cells. J. Biosci. 2011, 36, 215–221.
- Tsuchida, E. Blood Substitutes Present and Future Perspectives; Elsevier: Amsterdam, Netherlands, 1998.
- Simoni, J. Artificial oxygen carriers: Renewed commercial interest and scientific/technological advances. Artif. Organs 2012, 36, 123–126.
- Wong, S.S. Chemistry of Protein Conjugation and Cross-Linking; Chemical Rubber Company Press: Boca Raton, FL, USA, 1991.
- Deac, F.; Iacob, B.; Fischer-Fodor, E.; Damian, G.; Silaghi-Dumitrescu, R. Derivatization of haemoglobin with periodate-generated reticulation agents: Evaluation of oxidative reactivity for potential blood substitutes. J. Biochem. 2011, 149, 75–82.
- McGarrity, M.J.; Er, S.S.; Nightingale, K.A.; Hsia, J.C. ATP-hemoglobin: Anomalous oxygen binding properties. J. Chromatogr. 1987, 415, 136–142.
- Greenburg, A.G.; Maffuid, P.W. Modification of hemoglobin-ring opened dials. Prog. Clin. Biol. Res. I 1983, 122, 9–17.
- Hsia, C.J.; Er, S.S.; Hronowski, L.J.; Persaud, K.; Ansari, M.R. ATP-hemoglobin purification by ATP-agarose affinity chromatography. J. Chromatogr. 1986, 381, 153–157.
- Scurtu, F.; Zolog, O.; Iacob, B.; Silaghi-Dumitrescu, R. Hemoglobin-albumin crosslinking with disuccinimidyl suberate (DSS) and/or glutaraldehyde for blood substitutes. Artif. Cells Blood Substit. Immobil. Biotechnol. 2013. in press.
- Zolog, O.; Mot, A.; Deac, F.; Roman, A.; Fischer-Fodor, E.; Silaghi-Dumitrescu, R. A new polyethyleneglycol-derivatized hemoglobin derivative with decreased oxygen affinity and limited toxicity. Protein J. 2011, 30, 27–31.
- Veronese, F.M.; Pasut, G. PEGylation, successful approach. Drug Discov. Today 2005, 10, 1451–1458.
- Doherty, D.H.; Doyle, M.P.; Curry, S.R.; Vali, R.J.; Fattor, T.J.; Olson, J.S.; Lemon, D.D. Rate of reaction with nitric oxide determines the hypertensive effect of cell-free hemoglobin. Nat. Biotechnol. 1998, 16, 672–676.
- Weickert, M.J.; Pagratis, M; Glascock, C.B.; Blackmore, R. A mutation that improves soluble recombinant hemoglobin accumulation in Escherichia coli in heme excess. Appl. Environ. Microbiol. 1999, 62, 640–647.
- Brucker, E.A. Genetically crosslinked hemoglobin: A structuraly study. Acta Crystallogr. D Biol. Crystallogr. 2000, 56, 812–816.
- Bobofchak, K.M.; Mito, T; Texel, S.J.; Bellelli, A.; Nemoto, M.; Traystman, R.J.; Koehler, R.C.; Brinigar, W.S.; Fronticelli, C. A recombinant polymeric hemoglobin with conformational, functional, and physiological characteristics of an in vivo O2 transporter. Am. J. Physiol. Heart Circ. Physiol 2003, 285, H549–H561.
- Faggiano, S.; Bruno, S.; Ronda, L.; Pizzonia, P.; Pioselli, B.; Mozzarelli, A. Modulation of expression and polymerization of hemoglobin Polytaur, a blood substitute. Arc. Biochem. Biophys. 2011, 505, 42–47.
- Chang, T.S.M. Artificial cells with emphasis on cell encapsulation of genetically engineered cells. Artif. Organs 1998, 22, 958–965.
- Bradley, A.J.; Devine, D.V.; Ansell, S.M.; Janzena, J.; Brooks, D.E. Inhibition of liposome-induced complement activation by incorporated poly (ethylene glycol) lipids. Arch. Biochem. Biophys. 1998, 354, 185–194.
- Szebeni, J.; Baranyi, L.; Savay, S.; Bodo, M.; Morse, D.S.; Basta, M.; Stahl, G.L.; Bünger, R.; Alving, C.R. Liposome-induced pulmonary hypertension: properties and mechanism of a complement-mediated pseudoallergic reaction. Am. J. Physiol. Heart Circ. Physiol. 2000, 279, 1319–1328.
- Benz, R.; Elbert, R.; Prass, W.; Ringsdorf, H. Polymerization in black lipid membranes. Eur. Biophys. J. 1986, 14, 83–92.
- Iacob, B.; Deac, F.; Cioloboc, D.; Damian, G.; Silaghi-Dumitrescu, R. Hemoglobin-albumin crosslinked copolymers: Reduced prooxidant reactivity. Artif. Cells Blood Substit. Immobil. Biotechnol. 2011, 39, 293–297.
- Farmer, C.S.; Kurtz, D.M., Jr.; Phillips, R.S.; Ai, J.; Sanders-Loehr, J. A leucine residue “Gates” solvent but not O2 acces to the binding pocket of phascolopsis Gouldii hemerythrin. J. Biol. Chem. 2000, 275, 17043–17050.
- Mot, A.C.; Roman, A.; Kurtz, D.M.; Silaghi-Dumitrescu, R. Towards the development of hemerythrin-based blood substitutes. Protein J. 2010, 29, 387–393.
- Jin, S.; Kurtz, D.M., Jr.; Liu, Z.J.; Rose, J.; Wang, B.C. X-ray crystal structures of reduced rubrerythrin and its azide adduct: A structure-based mechanism for a non-heme diiron peroxidase. J. Am. Chem. Soc. 2002, 124, 9845–9855.
- Isaza, C.E.; Silaghi-Dumitrescu, R.; Iyer, R.B.; Kurtz, D.M., Jr.; Chan, M.K. Structural basis for O2 sensing by the hemerythrin-like domain of a bacterial chemotaxis protein: Substrate tunnel and fluxional N terminus. Biochemistry 2006, 45, 9023–9031.
© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).