- freely available
Pharmaceuticals 2013, 6(3), 340-357; doi:10.3390/ph6030340
Published: 19 March 2013
Abstract: The potential to emulate or enhance antibodies with nucleic acid aptamers while lowering costs has prompted development of new aptamer-protein, siRNA, drug, and nanoparticle conjugates. Specific focal points of this review discuss DNA aptamers covalently bound at their 3' ends to various proteins for enhanced stability and greater pharmacokinetic lifetimes in vivo. The proteins can include Fc tails of IgG for opsonization, and the first component of complement (C1q) to trigger complement-mediated lysis of antibiotic-resistant Gram negative bacteria, cancer cells and possibly some parasites during vulnerable stages. In addition, the 3' protein adduct may be a biotoxin, enzyme, or may simply be human serum albumin (HSA) or a drug known to bind HSA, thereby retarding kidney and other organ clearance and inhibiting serum exonucleases. In this review, the author summarizes existing therapeutic aptamer conjugate categories and describes his patented concept for PCR-based amplification of double-stranded aptamers followed by covalent attachment of proteins or other agents to the chemically vulnerable overhanging 3' adenine added by Taq polymerase. PCR amplification of aptamers could dramatically lower the current $2,000/gram cost of parallel chemical oligonucleotide synthesis, thereby enabling mass production of aptamer-3'-protein or drug conjugates to better compete against expensive humanized monoclonal antibodies.
Polyclonal antibodies or antisera have long been used as simple passive neutralizing agents for toxins or venoms. More recently humanized monoclonal antibodies have become popular for their many benefits as bio-therapeutic agents or specific “magic bullets.” Unfortunately, xenogeneic and allogeneic antisera can lead to serum sickness or anaphylactic shock, if used in vivo more than once  and humanized monoclonal antibodies are very expensive due to their long and arduous development cycles [2,3,4]. In addition, even fully “humanized” monoclonal antibodies can be immunogenic especially in their complementarity determining regions (CDRs) or hypervariable antigen binding sites .
Antibodies and their more recent molecular “cousins” the nucleic acid-based aptamers generally cannot kill target cells by themselves. Antibodies and aptamers merely bind to target cells or molecules against which they are developed with high affinity and specificity and either gain entry to the cell via receptor-mediated endocytosis  or peptide-mediated cell entry mechanisms [7,8,9] or mark the target cell for surface attack. It is, of course, the Fc tail of an antibody or a conjugated toxic molecule on an antibody or aptamer which brings about target cell destruction in the form of enhanced phagocytosis or opsonization , complement-mediated lysis [11,12,13,14,15], inhibition of protein synthesis , or other lethal mechanisms. These facts enable a bio-molecular engineer to couple antibodies or aptamers to a variety of toxic molecules or other effectors such as drugs [17,18,19], radioisotopes [20,21], phototoxic dyes and quantum dots [22,23,24,25,26,27,28,29] and various other nanoparticles [30,31,32,33,34,35,36,37] or small interfering RNA (siRNA) molecules [38,39,40,41,42] to achieve target cell destruction via the conjugate alone or in conjunction with physical forces including light and microwaves. This article summarizes many of the well-known methods for producing cytotoxic aptamer conjugates, but also focuses on lesser known DNA aptamer-3'-protein [13,43,44] and drug (e.g., ibuprofen, naproxen, etc.) conjugates in order for the aptamer-drug conjugates to bind and “hitch a ride” on protective albumins and other proteins in serum [45,46,47,48,49,50].
The advent of humanized monoclonal antibodies (hu mAbs) such as Herceptin® for breast cancer treatment has validated the efficacy of immunotherapy [14,51,52]. Unfortunately, the retail cost of humanized monoclonals to patients is nearly prohibitive [2,3,4] and their development is extremely long and arduous, which may account for the high cost. Various aptamer conjugates hold the promise of being relatively inexpensive, if produced by the Polymerase Chain Reaction (PCR; Figure 1), and highly selective “magic bullet” alternatives for immunotherapy. Aptamer-3'-Fc or C1q conjugates in particular could be developed rapidly against tumor cells, parasites and viruses, because some of these agents mutate over time and the innate immune system may need to be redirected during the critical antibody development period prior to seroconversion (i.e., act as passive immunity “bridges to life” [10,11,53,54,55]). Unfortunately, while very attractive in theory, the continual rapid development of new aptamer sequences to bind changing epitopes on mutating target cells seems unlikely to receive FDA approval, except perhaps for terminal patients. Of course, antibody technology is faced with the same “moving target” mutational drift dilemma. Fortunately, this is not an immensely significant problem and most of the well-known cancer markers and pathogen surface antigens are fairly stable.
The potential importance of aptamer conjugates as therapeutic agents is underscored by a number of efforts in recent years, including the formation of a company called Altermune, LLC by Nobel Prize winner Kary Mullis who has patented  and demonstrated the use of aptamer-alpha gal conjugates to redirect the immune system to prevent inhalation anthrax  and potentially other diseases. In addition, numerous groups have demonstrated the prevention or retardation of bacterial and viral diseases or biotoxin effects using aptamers for passive immunity in vitro and in vivo [10,11,53,54,55,58,59]. Even NASA has at least postulated the use of aptamer technology on board future spacecraft to counteract the effects of lethal extraterrestrial “Andromeda strain” microbes or latent viruses in astronauts which may exert their pathogenic effects after astronauts are stressed in the microgravity environment of deep space for a prolonged period of time .
2. Strategies for Conferring Greater Stability and Pharmacokinetic Lifetimes to Aptamers
The largest historical obstacle to the widespread use of aptamers and their predecessors (antisense oligonucleotides) as therapeutic agents has been their stability in vivo. The problem is really two-fold in nature: (1) DNA and RNA are susceptible to exonucleases and endonucleases in blood and other body fluids, and (2) because of their small size (generally about 25–30 kD), aptamers are rapidly cleared from circulation by the kidneys and other major organs, thereby drastically reducing their pharmacokinetic lifetime and therapeutic potential .
Several different biochemical approaches have been taken to enhancing the lifetime of aptamers in vivo. One approach has been to modify the phosphate backbone with sulfur atoms in place of some oxygen atoms to produce phosphorothioates or the so called “thio aptamers”  Another strategy has been to modify key sites on the sugar moieties with 2'-fluoro or 2'-O-methyl groups to confer resistance to nucleases . Likewise, blockage of the 3' or 5' ends of aptamers with small molecules such as biotin or the use of inverted bases on the ends has been semi-successful at retarding the enzymatic degradation due to serum nucleases and these modifications are used in the one FDA-approved anti-VEGF aptamer called Macugen®. The limitations to these modifications are that the modified sugars, especially if internal to the aptamer chain and associated with a binding site, may affect binding affinity or specificity toward the target. An interesting alternative to these chemical modifications is the RNA “Spiegelmer” (German for “mirror oligomer”) which utilizes the L-enantiomer of ribose to inhibit nuclease digestion [64,65]. While aptamer-peptide conjugates have the potential to facilitate aptamer entry into target cells [7,8,9], such conjugates do not add much weight. In fact, none of the chemical modifications or alternative strategies for enhancement of aptamer stability described thus far addresses the need for larger size or greater mass to avoid rapid renal or other organ clearance.
To address the size issue, some groups have added innocuous polyethylene glycol (PEG) to one end of an aptamer [66,67,68]. Dougan et al.  added streptavidin to the 3'-biotinylated ends of aptamers to block the main exonuclease in serum (Exonuclease I), thus extending the lifetimes of aptamers in vivo while adding significant mass to slow renal clearance. The author's group has added functional proteins to its aptamer-protein chimeras (dubbed “oligoteins,” Figure 1) such as the Fc tail of IgG for opsonization  or C1q for induction of complement-mediated lysis [11,12,13,15,44] of thin-walled target cells (Figure 2). Since the membrane attack complex (MAC) which results from complement activation and inserts deadly pores in target cells is only about 15 nm deep, it cannot kill Gram positive bacteria which can possess cell walls up to 80 nm thick, but the MAC can kill Gram negative antibiotic-resistant bacteria (a major cause of sepsis-related deaths; Figure 3 and Figure 4) . Aptamer induction of MAC pores could also kill cancer cells [12,15] and some types of parasites during susceptible phases of their life cycles  and when they emerge from their host cells.
The major barrier to killing cancer cells and some parasites by the aptamer-Fc or aptamer–C1q conjugate induction of the complement system is the innate ability of some cells to defeat complement activation. Cancer cells, in particular, possess membrane complement regulatory proteins (mCRPs such as CD46, CD55 and CD59) on their surfaces to ultimately thwart MAC pore formation and only allow for about 50%–60% cancer cell kill rates in vitro [12,14,15,44,70]. Perhaps the best way to counteract the effects of mCRPs will be to develop new aptamers against them to block their activity and enable higher kill rates in combination with aptamer-Fc or aptamer-C1q conjugates targeted to cancer-specific surface markers.
While some parasites such as Leishmania can be highly susceptible to complement-mediated lysis during certain stages of their life cycles (e.g., the promastigote stage) immediately after infection of the host’s blood, these same parasites can coat themselves in C3 protein from the complement cascade and erythrocytes to protect themselves and gain entry to phagocytes where they thrive . Many parasites also take on thicker and more resilient cell walls or plasma membranes in oocyst or cyst-like stages or are protected for long periods inside host cells. Hence, while there are brief windows of opportunity to kill parasites with aptamers by coupling to complement-mediated lysis, those windows are small and can be subverted by the parasites. Therefore, it seems more likely that aptamer-drug or other toxic payload conjugate endocytosis strategies would have a greater chance of success against most parasites.
3. General and Novel Attachment Chemistries
Nucleic acid-drug, protein or other molecular conjugation chemistry may seem trivial, but it often is not simple on the industrial scale. Numerous groups have developed and optimized small-scale conjugation approaches involving 3' or 5' primary amines coupled to various other molecules during chemical synthesis of nucleic acids or by classic carbodiimide , aldehyde [13,43,44], diazonium, or other approaches which take advantage of the much greater chemical reactivity of primary alkyl amine tags versus aryl amines on the nucleotides themselves.
While conjugation may be fairly straightforward on a small analytical scale or during chemical DNA synthesis, the scale-up to industrial mass production can be difficult, expensive and even prohibitive. Aptamers are generally inexpensive on the milligram synthesis scale ( ~50 cents per base). However, because chemical synthesis of DNA is governed by a 99% coupling efficiency for the growing oligonucleotide chain with yields dropping off greatly at greater than 70 bases (i.e., (0.99)7 = 0.495 or 49.5% yield), the commercial synthesis of lengthier ( >70 base) SELEX DNA templates was previously discouraged by oligonucleotide synthesis vendors. While shorter aptamers (60–70 bases) may be acceptable for small molecule (drug, dye, vitamin, etc.) targets, longer “linked” aptamers appear to be preferable, because they have demonstrated greater affinity, avidity and specificity for more complex antigens composed of several epitopes [73,74,75,76]. This is not surprising when one considers that nature has evolved 110 amino acid complementarity determining regions (CDRs) in antibody chains having three hypervariable binding sites linked together to form a multivalent binding molecule. Lengthier aptamers (up to 200 bases) are now being routinely synthesized on the mg scale. But, no aptamers can be mass produced at the gram-scale without expensive parallel chemical synthesis leading to a cost of ~$2,000 per gram .
This fact prompted the author's group to investigate a concept pioneered by Vandalia Research, Inc. (Vandalia, WV, USA) to mass produce aptamers and other DNA molecules cheaply by polymerase chain reaction (PCR) using their large-capacity Triathalon® PCR system . The enzymatic synthesis of DNA aptamers by use of PCR can clearly reduce the price of mass produced aptamers for the pharmaceutical industry, but the products are double-stranded (ds) and single-stranded (ss) aptamers are typically the active forms. The ds to ss conversion can be accomplished by heating or chemical means to break hydrogen bonds between the strands followed by affinity-based purification of the correct, functional, or “sense” strand. The author's group used the fact that double-stranded PCR products contain a chemically vulnerable unpaired overhanging adenine (A) on the 3' end to attach to proteins by various means including low levels of glutaraldehyde at cold temperatures [43,44]. This patented approach  could someday expand the use of lengthier in vivo stabilized aptamer-3'-protein conjugates by enabling their industrial scale mass production at reasonable costs.
4. Aptamer-Nanoparticle Conjugates
4.1. Aptamer-Magnetic Nanoparticle (MNP) Conjugates
Aptamers themselves are typically quite good at homing to their intended targets even in complex solutions including the in vivo milieu. But, magnetic nanoparticles can be used as the primary method of physically directing aptamer-MNPs and drug payloads to their target cells or tissues as well by use of an external magnetic field (“magnetic focusing”) which “drags” the MNPs and their complexes to any desired and accessible location in the body  where aptamer-MNPs could act as “nanosurgeons” . Superparamagnetic Iron Oxide Nanoparticles (SPIONs) consist of α-Fe2O3 (hematite), γ- Fe2O3 (maghemite) and Fe3O4 (magnetite) of 1-200 nm or greater diameters . SPIONs of 10-100 nm have the greatest lifetimes in vivo because they are large enough to avoid renal clearance, but small enough to evade phagocytosis . SPIONs currently have a large diversity of surface coatings  for chemical functionalization and aptamer attachment, if desired. MNPs can be used for magnetic hyperthermia (MHT) in conjunction with external alternating magnetic fields (AMFs) of 88.9 kHz to 13.56 MHz [80,81,82]. By comparison, most microwave ovens utilize radiofrequency radiation of 915 MHz or 2,450 MHz. The concept is to heat targeted tumors to between 41 °C and 43 °C because some types of tumor cells are more sensitive to such elevated temperatures than surrounding tissues which do not suffer much collateral damage [83,84]. These temperatures bring about a number of changes detrimental to the cell cycle and can induce apoptosis . Other types of NPs including gold NPs and quantum dots (QDs) have also been used in conjunction with radiofrequency (RF) radiation to kill tumors in vivo at least in animal models [86,87,88].
4.2. Aptamer-Quantum Dot (QD) and Photosensitizer Conjugates
As aforementioned, Quantum dots (QDs) and other nanoparticles can be used in RF fields to kill target cells. However, QDs themselves are composed of toxic metals such as cadmium and selenium and are therefore cytotoxic to a wide variety of prokaryotic and eukaryotic cells [22,23,24,25,89,90,91] even without an electromagnetic field. QDs appear to be even more toxic when brought in intimate contact with bacteria after conjugation to antibodies  (and presumably aptamers) or internalized by other target cells [90,91]. QDs are thought to be strong free radical (reactive oxygen species ROS; e.g., singlet oxygen) generators [22,26,27,28] especially when coupled with photosensitizer ligand dyes such as Rose Bengal or chlorin e6 [92,93]. Regardless of the exact mechanisms, QDs are clearly potential cytotoxic components to pair with aptamers in highly lethal conjugates and QDs have the added advantage of enabling deep tissue imaging to determine if the conjugate has reached its target in vivo .
Ferreira, et al.  have demonstrated a greater than 500-fold increase in killing of cancerous epithelial cells with a mucin-specific aptamer labeled with chlorin e(6) at its 5' end when excited by a helium cadmium laser. The work of Ferreira, et al.  and others has thus made the QD component unnecessary and paved the way for a simple class of aptamer-dye conjugates useful against a variety of superficial cancers that are accessible to light even deep in the body (e.g., mucosa of the gastrointestinal tract) when fiber optics are used.
4.3. Aptamer-Carbon Nanotube (CNT) Conjugates
A class of nanoparticles that clearly deserves consideration for incorporation into aptamer conjugates is that of carbon nanotubes (CNTs). Liu et al.  have reported the remarkable ability of single-walled carbon nanotubes (SWCNTs) to lance and kill bacteria in a concentration-dependent manner by themselves when shaken to cause collisions between the bacteria and SWCNTs. It therefore follows that aptamer attachment to SWCNTs might better direct the impaling action of SWCNTs. One can even envision “nanobots” (microscopic “robots”) capable of specific aptamer-based attachment to target cells followed by ATP hydrolysis-driven insertion of CNTs via “nano-motors” to kill target cells by osmotic damage much as the immune system directs complement-mediated lysis via MAC pores . Additionally, the hollow lumens of CNTs can be loaded with drug or toxin molecules for slow timed release of drugs  or perhaps even osmotically pumped into target cells when backed by a hypotonic fluid environment or some other thermodynamically favorable pumping mechanism.
4.4. Aptamer-Gold Nanoparticle Conjugates
Although used more for detection and diagnosis, several groups have reported various therapeutic schemes for using aptamer-gold and silver nanorods or gold nano “popcorn” having “hot spots” at the ends of the popcorn spikes for targeted photothermal killing of cancer cells [30,32,33,96,97]. These approaches have two major advantages over aptamer-QD conjugate systems. First, gold nanoparticles are generally not very toxic in vivo, at least not when compared to QDs. Second, 3' or 5' thiol-functionalized aptamers are readily available to enable facile spontaneous conjugation to gold nanoparticles with size-exclusion chromatography or other simple purification methods. The natural affinity of sulfur for gold makes chemical attachment methods quite facile when compared to the more complex attachment chemistries used for nucleic acids and functionalized QD shells. A third, lesser known, advantage to using aptamer-coated gold or other nanoparticles which Professor Tan's group at the University of Florida has astutely highlighted in the literature is that of increased collective affinity (~26-fold increased affinity when a nanoparticle is used versus the individual aptamer's measured affinity) . The classic immunologist may refer to this increased collective affinity as “avidity.” Regardless of the nomenclature, the effect of enhanced affinity is a very good aspect for future therapeutic potential of aptamer-nanoparticle conjugates.
4.5 Aptamer-Chitosan Nanoparticle Conjugates
Chitosan and its family of derivatives (chitosans) originate from chitin, the very tough polymer of N-acteyl-d-glucosamine which confers great strength to insect and shellfish exoskeletons. The highly cationic nature of chitosans can disrupt cell membranes making chitosan nanoparticles cytotoxic . This same highly positive charge density also makes chitosans very potentially useful for drug and aptamer delivery [99,100]. Indeed, chitosans and chitosan nanoparticles have long been investigated for their potential to cross the blood-brain barrier, if properly targeted using magnetic nanoparticles , antibodies, or possibly aptamers .
5. Aptamer-Drug and Toxin Conjugates
Numerous groups have demonstrated that certain receptor-binding aptamers can greatly aid internalization of drugs such as doxorubicin into cancer cells via endocytosis [6,16,17,18,19]. Chu et al. demonstrated several years ago that they could effectively kill prostate cancer cells in vitro using aptamer-carbodiimide-gelonin conjugates to inhibit protein synthesis in target cells . Hence, aptamer-drug conjugates are currently the subjects of intense study.
An interesting novel approach which is just beginning to emerge is the conjugation of simple drugs such as ibuprofen and naproxen known to bind serum albumins in the blood to aptamers to enable the aptamers to “hitch” an exonuclease-protected “ride” on albumin with the added benefit of increased mass for the overall aptamer-drug-albumin conglomerate [45,46,47,48,49]. The postulated aptamer-drug conjugate may also be able to offload from the albumin carrier at sites of inflammation. The same effects could be accomplished with aptamer-cholesterol conjugates and cholesterol binding proteins in the blood. Although, frequent and high dose administration of any conjugates containing cholesterol seems ill-advised for other health reasons.
6. Other Aptamer Conjugates
There are numerous other approaches to aptamer conjugation which may be of therapeutic value. This “miscellaneous” category includes aptamer-radioisotope conjugates [20,21], aptamer-drug-loaded liposomes  or high-capacity drug-carrying dendritic structures [104,105], and the aforementioned aptamer-siRNA conjugates [38,39,40,41,42]. The aptamer-siRNA category is particularly interesting from the standpoint that either an RNA aptamer can be extended to include the small interfering RNA or the siRNA can hybridize to one end of a DNA aptamer for potential future release inside a target cell [38,42]. Such “conjugates” or chimeras can be easily and naturally formed by transcription or simple hybridization. One problem with the aptamer-siRNA conjugate approach has been molecular engineering of each component to avoid inhibition or loss of either the aptamer’s or siRNA’s functionality due to hybridization between the components. Very recently Berezhnoy et al.  have reported that fusion of a siRNA to the 3' end of a DNA aptamer minimized or eliminated such negative effects on each component of the conjugate.
7. Conclusions and Future Directions
In this final section, some interesting emerging directions for aptamer and aptamer conjugate development and improvement are discussed. While this review touched on aptamer-siRNA conjugates, it has yet to mention the most obvious aptamer conjugate embodiment which is that of an aptamer being conjugated to another aptamer, wherein one aptamer may be used for targeting and the other to bring about some therapeutic effect. The so called bivalent or multivalent aptamer is beginning to emerge in the literature and receive attention for its greater affinity [73,74,75,76] and potentially greater specificity against complex target “antigens” [74,106,107]. The argument for binding to complex antigens having several distinct epitopes is entirely logical in that a linked aptamer can bind various epitopes and thereby achieve synergistic increases in affinity and avidity or overall binding strength. In addition, when multiplied together, multivalent aptamer specificity for various binding sites leads to a much lower probability of binding the wrong or unintended target. By comparison to forensic or paternity DNA fingerprinting analysis, the probability of matching multiple alleles is multiplicative and leads to virtual certainty of matches. Similarly, the specificity of longer linked or multivalent aptamers [73,74,75,76,106,107] is sure to increase with every successful new aptamer binding site which is proximal to the previously bound epitope of a complex antigen. Of course, naturally selected lengthier (100–200 base) aptamers which could cover several epitopes at once on a complex antigen would be preferable to relying on luck to pick the correct shorter aptamers or aptamer binding sites to link together for optimal binding of complex antigens post-selection. Nature appears to have evolved the same strategy as multivalent aptamers in the form of antibody complementarity determining regions (CDRs) which are 110 amino acids in length and contain several hypervariable binding sites for complex antigens. Perhaps, future aptamer researchers and developers should take a lesson from nature and emulate the length of the CDR or surpass it, which is now quite possible with most oligonucleotide vendors which no longer limit oligonucleotide length to 70 bases.
Consideration of the plentiful amino acid monomer selection (20 amino acids diversity) versus the paucity of natural nucleotides (four nucleotides for DNA or RNA) as building blocks of antibody and aptamer polymers respectively brings to mind another future area for aptamer and aptamer conjugate improvement, namely introduction of exotic amino-, carboxyl-, thio- or other modified bases to expand the repertoire of binding possibilities. The incorporation of exotic or modified nucleotides has been accomplished for catalytic DNAzymes and some aptamers [108,109] whether by chemical synthesis or the use of substrate-permissive DNA polymerases lacking editing functions (e.g., Deep Vent exo-). However, incorporation of modified bases is almost certainly a dual-edged sword since DNA or RNA polymers containing exotic bases will undoubtedly be recognized as foreign or non-self entities by the immune system, thereby making normally non-immunogenic aptamers, much more immunogenic in vivo. Still, the prospect for improved binding or even cytotoxic activity from the aptamer itself, should drive this area of research.
One other aspect for potential future consideration especially for longer multivalent aptamers might be internal stabilization of aptamer secondary and tertiary structures. Aptamers should always be selected at or near the temperature of their intended use. Thus, any aptamer intended for therapeutic use should be affinity selected at 37 °C to maximize correct 3-dimensional conformations for target binding. However, even if selected at the optimal working temperature, aptamers may “collapse” to a lower energy conformational state during cold storage and not be able to regain their optimal folded conformation unless reheated and allowed to cool again. To avoid such potential thermally-induced conformational problems, future aptamer research and development of longer aptamers should focus on understanding aptamer 3-dimensional structures and then possibly stabilizing them with specific hydrogen or disulfide bonds as occurs in nature to stabilize protein tertiary and quaternary structures.
Although lacking a crystal ball, the author believes it is not difficult to predict that aptamer-drug, siRNA, and Fc conjugates are the most likely candidates to advance towards eventual FDA approval for treatment of a variety of maladies. This prediction is based on the relative ease of conjugate production with good quality control and obvious similarity of aptamer-Fc and some other conjugates to actual antibodies. FDA-approved pharmaceuticals must of course be efficacious and safe. While many of the more exotic aptamer-nanoparticle or QD conjugates described herein are highly effective and may have applications in more radical therapies, they may not be as safe as aptamer conjugates which more closely emulate natural antibodies and may therefore these more contrived and exotic conjugates may not gain FDA approval. The recent demise of Archemix Corporation, although quite disheartening to members of the nucleic acid therapeutics community, should not dampen enthusiasm for aptamers and their conjugates. At present, numerous aptamer clinical trials are in progress around the world [110,111]. Almost certainly a few of these aptamer trials will be successful and ultimately someday result in better health care.
The author’s referenced projects were funded by DTRA Contract No. W911SR-06-C-0059, NIH Contract Nos. 1R43AI058561-01, 1R43AI058423-01, and NSF Contract No. DMI-0319766.
Conflict of Interest
The author holds a financial stake in a company (www.OTCBiotech.com) which sells DNA aptamers and holds intellectual property on aptamer-3'-conjugate formation methods.
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