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Pharmaceuticals 2011, 4(7), 1052-1069; doi:10.3390/ph4071052
Article

Vasoinhibins Prevent Bradykinin-Stimulated Endothelial Cell Proliferation by Inactivating eNOS via Reduction of both Intracellular Ca2+ Levels and eNOS Phosphorylation at Ser1179

1,†,* , 1,†
,
1
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1
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1
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2
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2
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1
 and
1
1 Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM), Campus UNAM-Juriquilla, Querétaro 76230, Mexico 2 Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Del. Coyoacán, México, D.F., 04510, Mexico These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 29 April 2011 / Revised: 6 July 2011 / Accepted: 19 July 2011 / Published: 20 July 2011
(This article belongs to the Special Issue Angiogenesis Inhibitors)
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Abstract

Vasoinhibins, a family of antiangiogenic peptides derived from prolactin proteolysis, inhibit the vascular effects of several proangiogenic factors, including bradykinin (BK). Here, we report that vasoinhibins block the BK-induced proliferation of bovine umbilical vein endothelial cells. This effect is mediated by the inactivation of endothelial nitric oxide synthase (eNOS), as the NO donor DETA-NONOate reverted vasoinhibin action. It is an experimentally proven fact that the elevation of intracellular Ca2+ levels ([Ca2+]i) upon BK stimulation activates eNOS, and vasoinhibins blocked the BK-mediated activation of phospholipase C and the formation of inositol 1,4,5-triphosphate leading to a reduced release of Ca2+ from intracellular stores. The [Ca2+]i rise evoked by BK also involves the influx of extracellular Ca2+ via canonical transient receptor potential (TRPC) channels. Vasoinhibins likely interfere with TRPC-mediated Ca2+ entry since La3+, which is an enhancer of TRPC4 and TRPC5 channel activity, prevented vasoinhibins from blocking the stimulation by BK of endothelial cell NO production and proliferation, and vasoinhibins reduced the BK-induced increase of TRPC5 mRNA expression. Finally, vasoinhibins prevented the BK-induced phosphorylation of eNOS at Ser1179, a post-translational modification that facilitates Ca2+-calmodulin activation of eNOS. Together, our data show that vasoinhibins, by lowering NO production through the inhibition of both [Ca2+]i mobilization and eNOS phosphorylation, prevent the BK-induced stimulation of endothelial cell proliferation. Thus, vasoinhibins help to regulate BK effects on angiogenesis and vascular homeostasis.
Keywords: vasoinhibins; 16kDa-prolactin; bradykinin; endothelial nitric oxide synthase; calcium mobilization; transient receptor potential channels vasoinhibins; 16kDa-prolactin; bradykinin; endothelial nitric oxide synthase; calcium mobilization; transient receptor potential channels
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Thebault, S.; González, C.; García, C.; Zamarripa, D.A.; Nava, G.; Vaca, L.; López-Casillas, F.; De la Escalera, G.M.; Clapp, C. Vasoinhibins Prevent Bradykinin-Stimulated Endothelial Cell Proliferation by Inactivating eNOS via Reduction of both Intracellular Ca2+ Levels and eNOS Phosphorylation at Ser1179. Pharmaceuticals 2011, 4, 1052-1069.

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