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Endogenous Matrix-Derived Inhibitors of Angiogenesis
Pharmaceuticals 2011, 4(6), 782-793; doi:10.3390/ph4060782

Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells

1, 1,2, 2 and 1,*
1 Department of Vascular Biology, Institute of Development Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan 2 Department of Obstetrics and Gynecology, Jichi Medical University School of Medicine, Japan
* Author to whom correspondence should be addressed.
Received: 25 April 2011 / Revised: 23 May 2011 / Accepted: 30 May 2011 / Published: 31 May 2011
(This article belongs to the Special Issue Angiogenesis Inhibitors)
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Vasohibin-1 (VASH1) is a VEGF-inducible gene of endothelial cells (ECs) that acts as a negative feedback regulator of angiogenesis. To further characterize the function of VASH1, we transfected human VASH1 gene into the mouse EC line MS1, established stable VASH1 expressing clones, and determined gene alteration by cDNA microarray analysis. Among the various angiogenesis-related genes, vascular endothelial growth factor type 1 receptor (VEGFR-1) and its alternative spliced form, soluble VEGFR1 (sVEGFR-1), were found to be the most significantly down-regulated genes. Transient overexpression of VASH1 in human umbilical vein endothelial cells confirmed the down-regulation of VEGFR-1 and sVEGFR-1. sVEGFR-1 is a decoy receptor for VEGF and inhibits angiogenesis. Interestingly, when sVEGFR-1 was overexpressed in ECs, it inhibited the expression of VASH1 in turn. These results suggest that VASH1 and sVEGFR-1, two angiogenesis inhibitors, mutually balance their expressions in ECs.
Keywords: angiogenesis inhibitor; endothelial cell; VASH1; sVEGFR-1 angiogenesis inhibitor; endothelial cell; VASH1; sVEGFR-1
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Miyashita, H.; Suzuki, H.; Ohkuchi, A.; Sato, Y. Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells. Pharmaceuticals 2011, 4, 782-793.

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