Pharmaceuticals 2011, 4(1), 202-214; doi:10.3390/ph4010202
Review

Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening

Martin Cottet 1 , Orestis Faklaris 1 , Jurriaan M. Zwier 2 , Eric Trinquet 2 , Jean-Philippe Pin 1  and Thierry Durroux 1,* email
1 Institut de Génomique Fonctionnelle, CNRS, UMR 5203, Montpellier, France and INSERM, U. 661, Montpellier, France and Université Montpellier,1,2, Montpellier, France 2 CIS bio international, BP 84175, F-30204 Bagnols sur Cèze Cedex, France
* Author to whom correspondence should be addressed.
Received: 1 December 2010; in revised form: 23 December 2010 / Accepted: 24 December 2010 / Published: 18 January 2011
(This article belongs to the Special Issue GPCR Based Drug Discovery)
PDF Full-text Download PDF Full-Text [265 KB, uploaded 18 January 2011 17:02 CET]
Abstract: The identification of new drugs exhibiting reduced adverse side-effects constitutes a great challenge for the next decade. Various steps are needed to screen for good ligand candidates and one of them is the evaluation of their binding properties. New strategies based on fluorescence measurement constitute excellent alternatives to the traditional radioactive assays. Less hazardous, faster and cheaper, these methods also exhibit very good sensitivity and can be used on various biological models such as heterologous expression systems or native tissues.
Keywords: screening assay; fluorescence; lanthanides; time resolved FRET; GPCR

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Cottet, M.; Faklaris, O.; Zwier, J.M.; Trinquet, E.; Pin, J.-P.; Durroux, T. Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening. Pharmaceuticals 2011, 4, 202-214.

AMA Style

Cottet M, Faklaris O, Zwier JM, Trinquet E, Pin J-P, Durroux T. Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening. Pharmaceuticals. 2011; 4(1):202-214.

Chicago/Turabian Style

Cottet, Martin; Faklaris, Orestis; Zwier, Jurriaan M.; Trinquet, Eric; Pin, Jean-Philippe; Durroux, Thierry. 2011. "Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening." Pharmaceuticals 4, no. 1: 202-214.

Pharmaceuticals EISSN 1424-8247 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert