Exploring Wound-Healing Genomic Machinery with a Network-Based Approach
AbstractThe molecular mechanisms underlying tissue regeneration and wound healing are still poorly understood despite their importance. In this paper we develop a bioinformatics approach, combining biology and network theory to drive experiments for better understanding the genetic underpinnings of wound healing mechanisms and for selecting potential drug targets. We start by selecting literature-relevant genes in murine wound healing, and inferring from them a Protein-Protein Interaction (PPI) network. Then, we analyze the network to rank wound healing-related genes according to their topological properties. Lastly, we perform a procedure for in-silico simulation of a treatment action in a biological pathway. The findings obtained by applying the developed pipeline, including gene expression analysis, confirms how a network-based bioinformatics method is able to prioritize candidate genes for in vitro analysis, thus speeding up the understanding of molecular mechanisms and supporting the discovery of potential drug targets. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 310 KB)
Share & Cite This Article
Vitali, F.; Marini, S.; Balli, M.; Grosemans, H.; Sampaolesi, M.; Lussier, Y.A.; Cusella De Angelis, M.G.; Bellazzi, R. Exploring Wound-Healing Genomic Machinery with a Network-Based Approach. Pharmaceuticals 2017, 10, 55.
Vitali F, Marini S, Balli M, Grosemans H, Sampaolesi M, Lussier YA, Cusella De Angelis MG, Bellazzi R. Exploring Wound-Healing Genomic Machinery with a Network-Based Approach. Pharmaceuticals. 2017; 10(2):55.Chicago/Turabian Style
Vitali, Francesca; Marini, Simone; Balli, Martina; Grosemans, Hanne; Sampaolesi, Maurilio; Lussier, Yves A.; Cusella De Angelis, Maria G.; Bellazzi, Riccardo. 2017. "Exploring Wound-Healing Genomic Machinery with a Network-Based Approach." Pharmaceuticals 10, no. 2: 55.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.