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Pharmaceuticals 2017, 10(1), 13; doi:10.3390/ph10010013

Ablation of Protein Kinase CK2β in Skeletal Muscle Fibers Interferes with Their Oxidative Capacity

Institute of Biochemistry, Friedrich-Alexander University of Erlangen-Nuremberg, Fahrstrasse 17, 91054 Erlangen, Germany
Both authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Mathias Montenarh
Received: 3 December 2016 / Revised: 13 January 2017 / Accepted: 14 January 2017 / Published: 19 January 2017
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Abstract

The tetrameric protein kinase CK2 was identified playing a role at neuromuscular junctions by studying CK2β-deficient muscle fibers in mice, and in cultured immortalized C2C12 muscle cells after individual knockdown of CK2α and CK2β subunits. In muscle cells, CK2 activity appeared to be at least required for regular aggregation of nicotinic acetylcholine receptors, which serves as a hallmark for the presence of a postsynaptic apparatus. Here, we set out to determine whether any other feature accompanies CK2β-deficient muscle fibers. Hind limb muscles gastrocnemius, plantaris, and soleus of adult wildtype and CK2β-deficient mice were dissected, cross-sectioned, and stained histochemically by Gomori trichrome and for nicotinamide adenine dinucleotide (NADH) dehydrogenase and succinate dehydrogenase (SDH) enzymatic activities. A reduction of oxidative enzymatic activity was determined for CK2β-deficient muscle fibers in comparison with wildtype controls. Importantly, the CK2β-deficient fibers, muscle fibers that typically exhibit high NADH dehydrogenase and SDH activities, like slow-type fibers, showed a marked reduction in these activities. Altogether, our data indicate additional impairments in the absence of CK2β in skeletal muscle fibers, pointing to an eventual mitochondrial myopathy. View Full-Text
Keywords: protein kinase CK2; skeletal muscle; C2C12; myopathy protein kinase CK2; skeletal muscle; C2C12; myopathy
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MDPI and ACS Style

Eiber, N.; Simeone, L.; Hashemolhosseini, S. Ablation of Protein Kinase CK2β in Skeletal Muscle Fibers Interferes with Their Oxidative Capacity. Pharmaceuticals 2017, 10, 13.

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