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Preparation of a Nanosized As2O3/Mn0.5Zn0.5Fe2O4 Complex and Its Anti-Tumor Effect on Hepatocellular Carcinoma Cells
School of Basic Medical Science, Southeast University, Nanjing 210009, China
School of Clinical Medical Science, Southeast University, Nanjing 210009, China
* Author to whom correspondence should be addressed.
Received: 25 June 2009; in revised form: 17 August 2009 / Accepted: 3 September 2009 / Published: 4 September 2009
Abstract: Manganese-zinc-ferrite nanoparticles (Mn0.5Zn0.5Fe2O4, MZF-NPs) prepared by an improved co-precipitation method and were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and energy dispersive spectrometry (EDS). Then thermodynamic testing of various doses of MZF-NPs was performed in vitro. The cytotoxicity of the Mn0.5Zn0.5Fe2O4 nanoparticles in vitro was tested by the MTT assay. A nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex was made by an impregnation process. The complex’s shape, component, envelop rate and release rate of As2O3 were measured by SEM, EDS and atom fluorescence spectrometry, respectively. The therapeutic effect of nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia (MFH) on human hepatocelluar cells were evaluated in vitro by an MTT assay and flow cytometry. The results indicated that Mn0.5Zn0.5Fe2O4 and nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex were both prepared successfully. The Mn0.5Zn0.5Fe2O4 nanoparticles had powerful absorption capabilities in a high-frequency alternating electromagnetic field, and had strong magnetic responsiveness. Moreover, Mn0.5Zn0.5Fe2O4 didn’t show cytotoxicity in vitro. The therapeutic result reveals that the nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex can significantly inhibit the growth of hepatoma carcinoma cells.
Keywords: As2O3/Mn0.5Zn0.5Fe2O4 complex; preparation; anti-tumor; hepatocellular carcinoma cells
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MDPI and ACS Style
Zhang, J.; Zhang, D. Preparation of a Nanosized As2O3/Mn0.5Zn0.5Fe2O4 Complex and Its Anti-Tumor Effect on Hepatocellular Carcinoma Cells. Sensors 2009, 9, 7058-7068.
Zhang J, Zhang D. Preparation of a Nanosized As2O3/Mn0.5Zn0.5Fe2O4 Complex and Its Anti-Tumor Effect on Hepatocellular Carcinoma Cells. Sensors. 2009; 9(9):7058-7068.
Zhang, Jia; Zhang, Dongsheng. 2009. "Preparation of a Nanosized As2O3/Mn0.5Zn0.5Fe2O4 Complex and Its Anti-Tumor Effect on Hepatocellular Carcinoma Cells." Sensors 9, no. 9: 7058-7068.