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Sensors 2009, 9(7), 5702-5714; doi:10.3390/s90705702
Article

Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts

1,* , 1
,
1
,
1
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1
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2
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2
1 University of Nis, Faculty of Technology, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia 2 University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
* Author to whom correspondence should be addressed.
Received: 25 May 2009 / Revised: 26 June 2009 / Accepted: 15 July 2009 / Published: 16 July 2009
(This article belongs to the Section Biosensors)
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Abstract

The antioxidant activity ofwater, ethanol and methanol Hieracium pilosella L.extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH· free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH· radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59–244.16 mg GAE/g of dry extract) and total flavonoids (79.13–82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity.
Keywords: Hieracium pilosella L. (Asteraceae); antioxidant activity; extraction; total phenolic content; total flavonoids; HPLC determination Hieracium pilosella L. (Asteraceae); antioxidant activity; extraction; total phenolic content; total flavonoids; HPLC determination
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Stanojević, L.; Stanković, M.; Nikolić, V.; Nikolić, L.; Ristić, D.; Čanadanovic-Brunet, J.; Tumbas, V. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors 2009, 9, 5702-5714.

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