Next Article in Journal
Adaptive Momentum-Based Motion Detection Approach and Its Application on Handoff in Wireless Networks
Previous Article in Journal
Object-Based Integration of Photogrammetric and LiDAR Data for Automated Generation of Complex Polyhedral Building Models
Sensors 2009, 9(7), 5702-5714; doi:10.3390/s90705702
Article

Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts

1,* , 1
,
1
,
1
,
1
,
2
 and
2
1 University of Nis, Faculty of Technology, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia 2 University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
* Author to whom correspondence should be addressed.
Received: 25 May 2009 / Revised: 26 June 2009 / Accepted: 15 July 2009 / Published: 16 July 2009
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [567 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

The antioxidant activity ofwater, ethanol and methanol Hieracium pilosella L.extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH· free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH· radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59–244.16 mg GAE/g of dry extract) and total flavonoids (79.13–82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity.
Keywords: Hieracium pilosella L. (Asteraceae); antioxidant activity; extraction; total phenolic content; total flavonoids; HPLC determination Hieracium pilosella L. (Asteraceae); antioxidant activity; extraction; total phenolic content; total flavonoids; HPLC determination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Stanojević, L.; Stanković, M.; Nikolić, V.; Nikolić, L.; Ristić, D.; Čanadanovic-Brunet, J.; Tumbas, V. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors 2009, 9, 5702-5714.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert