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Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts
University of Nis, Faculty of Technology, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia
University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
* Author to whom correspondence should be addressed.
Received: 25 May 2009; in revised form: 26 June 2009 / Accepted: 15 July 2009 / Published: 16 July 2009
Abstract: The antioxidant activity ofwater, ethanol and methanol Hieracium pilosella L.extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH· free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH· radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59–244.16 mg GAE/g of dry extract) and total flavonoids (79.13–82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity.
Keywords: Hieracium pilosella L. (Asteraceae); antioxidant activity; extraction; total phenolic content; total flavonoids; HPLC determination
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Stanojević, L.; Stanković, M.; Nikolić, V.; Nikolić, L.; Ristić, D.; Čanadanovic-Brunet, J.; Tumbas, V. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors 2009, 9, 5702-5714.
Stanojević L, Stanković M, Nikolić V, Nikolić L, Ristić D, Čanadanovic-Brunet J, Tumbas V. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors. 2009; 9(7):5702-5714.
Stanojević, Ljiljana; Stanković, Mihajlo; Nikolić, Vesna; Nikolić, Ljubiša; Ristić, Dušica; Čanadanovic-Brunet, Jasna; Tumbas, Vesna. 2009. "Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts." Sensors 9, no. 7: 5702-5714.