Sensors 2009, 9(7), 5702-5714; doi:10.3390/s90705702
Article

Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts

Received: 25 May 2009; in revised form: 26 June 2009 / Accepted: 15 July 2009 / Published: 16 July 2009
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The antioxidant activity ofwater, ethanol and methanol Hieracium pilosella L.extracts is reported. The antioxidative activity was tested by spectrophotometrically measuring their ability to scavenge a stable DPPH· free radical and a reactive hydroxyl radical trapped by DMPO during the Fenton reaction, using the ESR spectroscopy. Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. A HPLC method was used for identification of some phenolic compounds (chlorogenic acid, apigenin-7-O-glucoside and umbelliferone). The antioxidant activity of the investigated extracts slightly differs depending on the solvent used. The concentration of 0.30 mg/mL of water, ethanol and methanol extract is less effective in scavenging hydroxyl radicals (56.35, 58.73 and 54.35%, respectively) in comparison with the DPPH· radical scavenging activity (around 95% for all extracts). The high contents of total phenolic compounds (239.59–244.16 mg GAE/g of dry extract) and total flavonoids (79.13–82.18 mg RE/g of dry extract) indicated that these compounds contribute to the antioxidative activity.
Keywords: Hieracium pilosella L. (Asteraceae); antioxidant activity; extraction; total phenolic content; total flavonoids; HPLC determination
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MDPI and ACS Style

Stanojević, L.; Stanković, M.; Nikolić, V.; Nikolić, L.; Ristić, D.; Čanadanovic-Brunet, J.; Tumbas, V. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors 2009, 9, 5702-5714.

AMA Style

Stanojević L, Stanković M, Nikolić V, Nikolić L, Ristić D, Čanadanovic-Brunet J, Tumbas V. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors. 2009; 9(7):5702-5714.

Chicago/Turabian Style

Stanojević, Ljiljana; Stanković, Mihajlo; Nikolić, Vesna; Nikolić, Ljubiša; Ristić, Dušica; Čanadanovic-Brunet, Jasna; Tumbas, Vesna. 2009. "Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts." Sensors 9, no. 7: 5702-5714.

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